Journal: Frontiers in Immunology

Article Title: Protective role of complement factor H against the development of preeclampsia

doi: 10.3389/fimmu.2024.1351898

Figure Lengend Snippet: (A, B) Measurement of circulating Factor H (FH) levels in sera of normal (CTRL) and preeclamptic (PE) women, collected during third (A) or first (B) trimester, by ELISA. During both the first trimester and the third trimester lower levels of FH were observed in PE pregnancies compared to CTRL, showing a highly significant difference ( n = 26 PE vs n =31 CTRL sera were compared for third trimester of pregnancy; n = 20 PE vs n = 20 CTRL for first trimester of pregnancy). * p < 0.05, **** p < 0.0001 (t-test). (C, D) Evaluation of FH deposition on placental STBMs via Western blot. STBMs derived from PE ( n = 4) and CTRL ( n = 4) were solubilized and separated by SDS-PAGE under reducing conditions. After transfer, the membrane was probed with anti-FH antibody and IRDye 800CW secondary antibody. Signal intensity was detected using an Odyssey CLx near-infrared scanner (LI-COR Biosciences, Lincoln, NE, USA). Image acquisition, processing and data analysis were performed with Image Studio 5.2 (LI-COR Biosciences). Histograms represent the mean ± SD of two independent experiments performed in duplicate. Beta-actin (ACTB) was used to normalize the results. * p < 0.05 (Mann-Whitney test).

Article Snippet: Human Complement FH DuoSet ELISA kit (#DY4779; R&D Systems, Inc., Minneapolis, Canada) was used to quantify FH in serum samples, following the manufacturer's instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Derivative Assay, SDS Page, Membrane, MANN-WHITNEY

Journal: Scientific Reports

Article Title: Complement factor H binding of monomeric C-reactive protein downregulates proinflammatory activity and is impaired with at risk polymorphic CFH variants

doi: 10.1038/srep22889

Figure Lengend Snippet: Various amounts of purified mCRP and pCRP were immobilized on a microtiter plate and serum (1;1,000 diluted) from AMD patients homozygous for the CFH H402 or CFH Y402 variants was added. Bound FH was detected with a polyclonal anti-human FH antibody. Notice that mCRP (solid lines), but not pCRP (dashed lines), bound both FH variants in a dose-dependent manner. Further, mCRP bound tighter to the non-risk FH variant (filled circles, solid line) compared with the risk variant (filled squares, solid line) (n = 3). * P < 0.05 vs. pCRP, # P < 0.05 vs. mCRP-FH Y402 .

Article Snippet: Biotinylated polyclonal anti-human FH (AssayPro human FH ELISA kit) was added, followed by the addition of streptavidin-peroxidase conjugate (AssayPro human FH ELISA kit).

Techniques: Purification, Variant Assay