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cd73 (nt5e) antibody, anti-human, readye_lease  (Miltenyi Biotec)


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    Miltenyi Biotec cd73 (nt5e) antibody, anti-human, readye_lease
    Cd73 (Nt5e) Antibody, Anti Human, Readye Lease, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+cd73/CD73+(NT5E)+Antibody%2C+anti-human%2C+REAdye_lease/custom%40130-129-891%4042502392
    Average 94 stars, based on 1 article reviews
    cd73 (nt5e) antibody, anti-human, readye_lease - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    other:

    Article Title: Generation of human induced pluripotent stem cell-derived liver buds with chemically defined and animal origin-free media
    Article Snippet: Following antibodies were used for flow cytometric analysis: PE-conjugated mouse anti-human CD140b (PDGFRB; 558821, BD Biosciences); APC-conjugated anti-human CD166 (ALCAM; 130-119-809, Miltenyi Biotec); anti- human CD105, human CD73, and human CD90 (MSC phenotyping kit; 130-095-198, Miltenyi Biotec); FITC-conjugated mouse anti-human CD31 (557508, BD Biosciences); PE-conjugated mouse anti-human CD144 (561714, BD Biosciences).

    Article Title: Additive Effect of CD73 Inhibitor in Colorectal Cancer Treatment With CDK4/6 Inhibitor Through Regulation of PD-L1
    Article Snippet: For the fluorescence-activated cell sorting analysis, cells were stained with APC,-anti human CD163 (clone: GHI/61), PD-anti human CTLA4 (clone: L3D10), Alexa 647-anti human IDO1 (clone: 2E2/IDO1), PE-anti human DR4 (clone: DJR1), FITC-anti human CD47 (clone: REA220, FITC-anti human MICA&B (clone: 6D4), PE-anti human PD-L1 (clone: MIH2), PD-anti human CD69 (clone: FNM50), FITC-anti human CD2 (clone: RPA-2.10), FITC-anti human CD20 (clone: 2H7), APC-anti mouse F4/80 (clone: BM8), Brilliant Violet 421 TM -anti mouse CD11b (clone: M1/70), Brilliant Violet 570 TM -anti mouse CD45 (clone: 104), PE-anti-human CD45 (clone: HI30), monoclonal antibodies (Biolegend; San Diego, CA, USA), FITC-anti-human CD40 (clone: REA733), PE-anti human CD80 (clone: 2D10), PE/vio770-anti human CD206 (clone: DCN228), PE-anti human CD62E (clone: REA280), PE-anti human CD192 (clone: REA264), APC-anti human I-CAM (clone: REA266), APC-anti human HLA-DR, DP, DQ (clone: REA332), APC-anti human CCL2 (clone: REA485), APC-anti human CD14 (clone: HI30), Vioblue-anti human CD31 (clone: TUK4), FTIC-anti-human CD86 (clone: FM95), PE-anti human CD73 (clone: AD2), PE-anti mouse PD-L1 (clone: 60533), PE-anti mouse CD39, and PE-anti human CD39 (clone: REA739) monoclonal antibodies (Miltenyi Biotec; Bergisch Gladbach, Germany), and PE/CF594-anti human CD3 (clone: UCHT1), PE-anti human CD44 (clone: G44-26), PE-anti human CD183 (clone: 150503), and PD-1 (clone: REA739) monoclonal antibodies (BD Biosciences; San Jose, CA, USA) following the manufacturer’s protocol.

    Article Title: Additive Effect of CD73 Inhibitor in Colorectal Cancer Treatment With CDK4/6 Inhibitor Through Regulation of PD-L1.
    Article Snippet: For the fluorescence-activated cell sorting analysis, cells were stained with APC,-anti human CD163 (clone: GHI/61), PD-anti human CTLA4 (clone: L3D10), Alexa 647-anti human IDO1 (clone: 2E2/IDO1), PE-anti human DR4 (clone: DJR1), FITC-anti human CD47 (clone: REA220, FITC-anti human MICA&B (clone: 6D4), PE-anti human PD-L1 (clone: MIH2), PD-anti human CD69 (clone: FNM50), FITCanti human CD2 (clone: RPA-2.10), FITC-anti human CD20 (clone: 2H7), APC-anti mouse F4/80 (clone: BM8), Brilliant Violet 421TM-anti mouse CD11b (clone: M1/70), Brilliant Violet 570TM-anti mouse CD45 (clone: 104), PE-anti-human CD45 (clone: HI30), monoclonal antibodies (Biolegend; San Diego, CA, USA), FITC-anti-human CD40 (clone: REA733), PE-anti human CD80 (clone: 2D10), PE/vio770-anti human CD206 (clone: DCN228), PE-anti human CD62E (clone: REA280), PE-anti human CD192 (clone: REA264), APC-anti human I-CAM (clone: REA266), APC-anti human HLA-DR, DP, DQ (clone: REA332), APC-anti human CCL2 (clone: REA485), APC-anti human CD14 (clone: HI30), Vioblue-anti human CD31 (clone: TUK4), FTIC-anti-human CD86 (clone: FM95), PE-anti human CD73 (clone: AD2), PE-anti mouse PD-L1 (clone: 60533), PE-anti mouse CD39, and PE-anti human CD39 (clone: REA739) monoclonal antibodies (Miltenyi Biotec; Bergisch Gladbach, Germany), and PE/CF594anti human CD3 (clone: UCHT1), PE-anti human CD44 (clone: G44-26), PE-anti human CD183 (clone: 150503), and PD-1 (clone: REA739) monoclonal antibodies (BD Biosciences; San Jose, CA, USA) following the manufacturer’s protocol.

    Article Title: Robust detection of undifferentiated iPSC among differentiated cells
    Article Snippet: Following antibodies were used for flow cytometric analysis: PE-conjugated mouse anti-human CD140b (PDGFRB; 558821, BD Biosciences); APC-conjugated anti-human CD166 (ALCAM; 130-119-809, Miltenyi Biotec); anti- human CD105, human CD73, and human CD90 (MSC phenotyping kit; 130-095-198, Miltenyi Biotec); FITC-conjugated mouse anti-human CD31 (557508, BD Biosciences); PE-conjugated mouse anti-human CD144 (561714, BD Biosciences).

    Flow Cytometry:

    Article Title: Immune recognition of syngeneic, allogeneic and xenogeneic stromal cell transplants in healthy retinas.
    Article Snippet: .. Mesenchymal phenotype of hBM-MSCs was verified by flow cytometry using specific antibodies for human CD73, CD90, CD105, CD14, CD20, CD34 and CD45 (human MSC phenotyping cocktail; Miltenyi Biotec, Bergisch Gladbach, Germany) in a FACS Canto flow cytometer (Becton Dickinson, Franklin Lakes, NJ, USA) as previously reported [18] (Additional file 1: Fig. S1A, left). ..

    Article Title: Immune recognition of syngeneic, allogeneic and xenogeneic stromal cell transplants in healthy retinas
    Article Snippet: .. Mesenchymal phenotype of hBM-MSCs was verified by flow cytometry using specific antibodies for human CD73, CD90, CD105, CD14, CD20, CD34 and CD45 (human MSC phenotyping cocktail; Miltenyi Biotec, Bergisch Gladbach, Germany) in a FACS Canto flow cytometer (Becton Dickinson, Franklin Lakes, NJ, USA) as previously reported [ ] (Additional file : Fig. S1A, left). ..

    FACS:

    Article Title: Immune recognition of syngeneic, allogeneic and xenogeneic stromal cell transplants in healthy retinas.
    Article Snippet: .. Mesenchymal phenotype of hBM-MSCs was verified by flow cytometry using specific antibodies for human CD73, CD90, CD105, CD14, CD20, CD34 and CD45 (human MSC phenotyping cocktail; Miltenyi Biotec, Bergisch Gladbach, Germany) in a FACS Canto flow cytometer (Becton Dickinson, Franklin Lakes, NJ, USA) as previously reported [18] (Additional file 1: Fig. S1A, left). ..

    Article Title: Immune recognition of syngeneic, allogeneic and xenogeneic stromal cell transplants in healthy retinas
    Article Snippet: .. Mesenchymal phenotype of hBM-MSCs was verified by flow cytometry using specific antibodies for human CD73, CD90, CD105, CD14, CD20, CD34 and CD45 (human MSC phenotyping cocktail; Miltenyi Biotec, Bergisch Gladbach, Germany) in a FACS Canto flow cytometer (Becton Dickinson, Franklin Lakes, NJ, USA) as previously reported [ ] (Additional file : Fig. S1A, left). ..

    Incubation:

    Article Title: Safety and Biodistribution of Human Bone Marrow-Derived Mesenchymal Stromal Cells Injected Intrathecally in Non-Obese Diabetic Severe Combined Immunodeficiency Mice: Preclinical Study
    Article Snippet: .. Briefly, cells were detached, rinsed twice with PBS and incubated in the dark at 4 °C for 30 min with fluorescence-conjugated specific monoclonal antibodies for human CD73, CD90 and CD105; Allophycocyanin (APC, Miltenyi Biotec, Bergisch Gladbach, Germany), Fluorescein isothiocyanate (FITC, Miltenyi Biotec, Bergisch Gladbach, Germany), Phycoerythrin (PE, Miltenyi Biotec, Bergisch Gladbach, Germany) respectively. .. Lack of expression of the hematopoietic markers CD14, CD20, CD34 and CD45 was also analyzed using Peridinin Chlorophyll-protein complex (PerCP, Miltenyi Biotec, Bergisch Gladbach, Germany).

    Fluorescence:

    Article Title: Safety and Biodistribution of Human Bone Marrow-Derived Mesenchymal Stromal Cells Injected Intrathecally in Non-Obese Diabetic Severe Combined Immunodeficiency Mice: Preclinical Study
    Article Snippet: .. Briefly, cells were detached, rinsed twice with PBS and incubated in the dark at 4 °C for 30 min with fluorescence-conjugated specific monoclonal antibodies for human CD73, CD90 and CD105; Allophycocyanin (APC, Miltenyi Biotec, Bergisch Gladbach, Germany), Fluorescein isothiocyanate (FITC, Miltenyi Biotec, Bergisch Gladbach, Germany), Phycoerythrin (PE, Miltenyi Biotec, Bergisch Gladbach, Germany) respectively. .. Lack of expression of the hematopoietic markers CD14, CD20, CD34 and CD45 was also analyzed using Peridinin Chlorophyll-protein complex (PerCP, Miltenyi Biotec, Bergisch Gladbach, Germany).

    Bioprocessing:

    Article Title: Safety and Biodistribution of Human Bone Marrow-Derived Mesenchymal Stromal Cells Injected Intrathecally in Non-Obese Diabetic Severe Combined Immunodeficiency Mice: Preclinical Study
    Article Snippet: .. Briefly, cells were detached, rinsed twice with PBS and incubated in the dark at 4 °C for 30 min with fluorescence-conjugated specific monoclonal antibodies for human CD73, CD90 and CD105; Allophycocyanin (APC, Miltenyi Biotec, Bergisch Gladbach, Germany), Fluorescein isothiocyanate (FITC, Miltenyi Biotec, Bergisch Gladbach, Germany), Phycoerythrin (PE, Miltenyi Biotec, Bergisch Gladbach, Germany) respectively. .. Lack of expression of the hematopoietic markers CD14, CD20, CD34 and CD45 was also analyzed using Peridinin Chlorophyll-protein complex (PerCP, Miltenyi Biotec, Bergisch Gladbach, Germany).

    Single Cell:

    Article Title: Defects in Long-Term APC Repopulation Ability of Adult Human Bone Marrow Hematopoietic Stem Cells (HSCs) Compared with Fetal Liver HSCs.
    Article Snippet: .. Fc receptors in single-cell suspensions were blocked with anti-mouse Fc receptor mAb (2.4G2) and 10% human AB serum (Gemini) and stained with fluorophore-conjugated Abs against mouse TER119 (Ter119), mouse CD45 (30-F11), human CD45 (H130), HLA-DR (G46-6), mouse/human CD11b (M1/70), mouse/human CD11c (B-ly6), human CD123 (9F5) (from BD Pharmingen); human CD45RA (MEM-56), human CD14 (61D3), human CD73 (AD2), human CD90 (5E10), human CD105 (SN6), human CD34 (581), human CD38 (HIT2), human CD10 (HI10a), human CD19 (HIB19), human CD115 (9- 4D2-1E4), human CD123 (6H6) (from eBioscience); and anti-human CD303 (AC144) (from Miltenyi Bioscience). ..

    Staining:

    Article Title: Defects in Long-Term APC Repopulation Ability of Adult Human Bone Marrow Hematopoietic Stem Cells (HSCs) Compared with Fetal Liver HSCs.
    Article Snippet: .. Fc receptors in single-cell suspensions were blocked with anti-mouse Fc receptor mAb (2.4G2) and 10% human AB serum (Gemini) and stained with fluorophore-conjugated Abs against mouse TER119 (Ter119), mouse CD45 (30-F11), human CD45 (H130), HLA-DR (G46-6), mouse/human CD11b (M1/70), mouse/human CD11c (B-ly6), human CD123 (9F5) (from BD Pharmingen); human CD45RA (MEM-56), human CD14 (61D3), human CD73 (AD2), human CD90 (5E10), human CD105 (SN6), human CD34 (581), human CD38 (HIT2), human CD10 (HI10a), human CD19 (HIB19), human CD115 (9- 4D2-1E4), human CD123 (6H6) (from eBioscience); and anti-human CD303 (AC144) (from Miltenyi Bioscience). ..



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    Image Search Results


    (a) Immunofluorescence staining showing expression of Vimentin (green) and CD105 (red) in DPSC and SHED compared with HaCaT epithelial controls. (b) STRO-1 (green) immunostaining in SHED. Nuclei were counterstained with DAPI (blue). Scale bar = 100μm (c) Representative flow cytometry analyses demonstrating expression of mesenchymal stem cell markers (CD73, CD90, CD105, HLA-ABC) and absence of hematopoietic markers (CD34, CD45). All experiments were perfomed at passage 5 (d) Growth curves comparing cumulative population doublings of DPSC and SHED derived from four independent donors across serial passages. SHED displayed enhanced proliferative capacity and reduced donor-to-donor variability.

    Journal: bioRxiv

    Article Title: Human SHED-derived extracellular cues activate a specialized neuroprotective and regenerative program in developing retinal ganglion cells

    doi: 10.64898/2026.06.25.733625

    Figure Lengend Snippet: (a) Immunofluorescence staining showing expression of Vimentin (green) and CD105 (red) in DPSC and SHED compared with HaCaT epithelial controls. (b) STRO-1 (green) immunostaining in SHED. Nuclei were counterstained with DAPI (blue). Scale bar = 100μm (c) Representative flow cytometry analyses demonstrating expression of mesenchymal stem cell markers (CD73, CD90, CD105, HLA-ABC) and absence of hematopoietic markers (CD34, CD45). All experiments were perfomed at passage 5 (d) Growth curves comparing cumulative population doublings of DPSC and SHED derived from four independent donors across serial passages. SHED displayed enhanced proliferative capacity and reduced donor-to-donor variability.

    Article Snippet: Antibodies against the following human antigens were used: CD105-FITC (Miltenyi Biotect, Bergisch Gladbach, Germany, cat# 130-112-327, 1:50), CD90-FITC (Miltenyi Biotec, cat# 130-114-901, 1:50), CD44-VioBlue (Miltenyi Biotec, cat# 130-113-906, 1:50), CD73-APC (Miltenyi Biotec, cat# 130-111-909, 1:50), MSC Phenotyping Cocktail-PE (CD34, CD14, CD19, CD45, Miltenyi Biotec cat# 130-125-285, dilution according to the manufacturer’s instructions).

    Techniques: Immunofluorescence, Staining, Expressing, Immunostaining, Flow Cytometry, Derivative Assay