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MT2A knockdown in hDPCs cuproptosis model enhances macrophage migration and M1 polarization, A. Pearson correlation coefficients among immune cells and the MT2A in human pulp tissues from GEO database ( GSE92681 and GSE77459 ); B .Immunofluorescence staining of CD68 protein in human healthy pulp tissues (n = 3) and pulpitis tissues (n = 3); C. Transwell assays detected the migration of macrophages (n = 3); D. Flow cytometry detected the polarization of macrophages by the expression of CD80 and CD163 (n = 3).

Journal: International Dental Journal

Article Title: MT2A-Mediated Regulation of Cuproptosis in Human Dental Pulp Cells Modulates Macrophage M1 Polarization in Pulpitis

doi: 10.1016/j.identj.2026.109506

Figure Lengend Snippet: MT2A knockdown in hDPCs cuproptosis model enhances macrophage migration and M1 polarization, A. Pearson correlation coefficients among immune cells and the MT2A in human pulp tissues from GEO database ( GSE92681 and GSE77459 ); B .Immunofluorescence staining of CD68 protein in human healthy pulp tissues (n = 3) and pulpitis tissues (n = 3); C. Transwell assays detected the migration of macrophages (n = 3); D. Flow cytometry detected the polarization of macrophages by the expression of CD80 and CD163 (n = 3).

Article Snippet: The cells were resuspended with 300 μL PBS and incubated with antibodies against human CD163 (PTM Bio) and CD80 (Affinity Biosciences) for 1 h on ice.

Techniques: Knockdown, Migration, Immunofluorescence, Staining, Flow Cytometry, Expressing

MTII knockdown enhances macrophage infiltration and M1 polarization in the mouse pulpitis model, A. Transwell assays detected the migration of macrophages (n = 3); B. Flow cytometry detected the polarization of macrophages by the expression of CD80 and CD163 (n = 3); C. IF staining of MTII, F4/80 and iNOS protein (n = 5).

Journal: International Dental Journal

Article Title: MT2A-Mediated Regulation of Cuproptosis in Human Dental Pulp Cells Modulates Macrophage M1 Polarization in Pulpitis

doi: 10.1016/j.identj.2026.109506

Figure Lengend Snippet: MTII knockdown enhances macrophage infiltration and M1 polarization in the mouse pulpitis model, A. Transwell assays detected the migration of macrophages (n = 3); B. Flow cytometry detected the polarization of macrophages by the expression of CD80 and CD163 (n = 3); C. IF staining of MTII, F4/80 and iNOS protein (n = 5).

Article Snippet: The cells were resuspended with 300 μL PBS and incubated with antibodies against human CD163 (PTM Bio) and CD80 (Affinity Biosciences) for 1 h on ice.

Techniques: Knockdown, Migration, Flow Cytometry, Expressing, Staining