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Metformin induces ZFP36 expression in cervical cancer-derived-cell lines. HeLa and CaSki cells were treated with metformin 20mM for 24 hours, then RNA was extracted for analysis by RT-qPCR. RT-qPCR of ZFP36 after metformin treatment in ( A ) HeLa and ( B ) CaSki. Representative Western Blot of ZFP36 and densitometric analysis in ( C ) HeLa and ( D ) CaSki, ( E ) HaCaT and ( F ) Human Uterine <t>Fibroblast</t> <t>(HUF).</t> Results are shown as means ± SD of at least three independent experiments. * p <0.05 **, p <0.01
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Metformin induces ZFP36 expression in cervical cancer-derived-cell lines. HeLa and CaSki cells were treated with metformin 20mM for 24 hours, then RNA was extracted for analysis by RT-qPCR. RT-qPCR of ZFP36 after metformin treatment in ( A ) HeLa and ( B ) CaSki. Representative Western Blot of ZFP36 and densitometric analysis in ( C ) HeLa and ( D ) CaSki, ( E ) HaCaT and ( F ) Human Uterine Fibroblast (HUF). Results are shown as means ± SD of at least three independent experiments. * p <0.05 **, p <0.01

Journal: BMC Cancer

Article Title: Metformin induces ZFP36 by mTORC1 inhibition in cervical cancer-derived cell lines

doi: 10.1186/s12885-024-12555-5

Figure Lengend Snippet: Metformin induces ZFP36 expression in cervical cancer-derived-cell lines. HeLa and CaSki cells were treated with metformin 20mM for 24 hours, then RNA was extracted for analysis by RT-qPCR. RT-qPCR of ZFP36 after metformin treatment in ( A ) HeLa and ( B ) CaSki. Representative Western Blot of ZFP36 and densitometric analysis in ( C ) HeLa and ( D ) CaSki, ( E ) HaCaT and ( F ) Human Uterine Fibroblast (HUF). Results are shown as means ± SD of at least three independent experiments. * p <0.05 **, p <0.01

Article Snippet: Primary Human Uterine Fibroblast (HUF) (PCS-460–010) and human cervical cancer-derived cell lines HeLa (RRID: CVCL_0030) and CaSki (RRID: CVCL_1100) were obtained from ATCC (Manassas, VA, USA).

Techniques: Expressing, Derivative Assay, Quantitative RT-PCR, Western Blot