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Effect of audiogenic kindling (AK) on messenger RNA (mRNA) expression in the basolateral (A1—D2) and central amygdala (E1—H2) and hippocampus (I1—L2) of serotonin post synaptic receptors 1A ( Htr1a ), 1B ( Htr1b ), 2A ( <t>Htr2a</t> ), and 2C ( Htr2c ) in Wistar and Wistar Audiogenic rats (WAR). These data were analyzed by Two‐way ANOVA followed by Tukey's post‐test or One‐way followed by the Tukey post‐test. # p < 0.05 WARs versus Wistars; *AK versus naive; +AK versus control; @WARs under AK versus Wistars. Box plots display the median (center line), interquartile range (box), and minimum/maximum values (whiskers). All individual data points are shown.
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(A) Representative image of <t>HTR2A</t> protein immunoblot with increasing amounts of total protein (10 μg, 12 μg, 14 μg, 16 μg, 18 μg, 20 μg). (B) Linear regression of pooled total protein extracted from bovine lateral saphenous veins with HTR2A protein expression. (B) Representative image of HTR2A protein immunoblot with increasing amounts of total protein. (C) Representative image of HTR2A protein immunoblot for quantitative protein expression. CON = modified Kreb’s-Henseleit buffer; 5HT = 5 × 10 −7 M 5-HT; ERV = 2.01 × 10 −7 M ERV; 5HT + ERV = 5 × 10 −7 M 5-HT and 2.01 × 10 −7 M ERV. (D) Least squares mean relative HTR2A protein expression ± SEM bovine lateral saphenous veins exposed to control, 5-HT, ERV, or 5-HT + ERV. Probability values for effect of 5-HT: P = 0.68, effect of ERV: P = 0.88, and the interaction of 5-HT and ERV: P = 0.54. ( n = 4).
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(A) Representative image of <t>HTR2A</t> protein immunoblot with increasing amounts of total protein (10 μg, 12 μg, 14 μg, 16 μg, 18 μg, 20 μg). (B) Linear regression of pooled total protein extracted from bovine lateral saphenous veins with HTR2A protein expression. (B) Representative image of HTR2A protein immunoblot with increasing amounts of total protein. (C) Representative image of HTR2A protein immunoblot for quantitative protein expression. CON = modified Kreb’s-Henseleit buffer; 5HT = 5 × 10 −7 M 5-HT; ERV = 2.01 × 10 −7 M ERV; 5HT + ERV = 5 × 10 −7 M 5-HT and 2.01 × 10 −7 M ERV. (D) Least squares mean relative HTR2A protein expression ± SEM bovine lateral saphenous veins exposed to control, 5-HT, ERV, or 5-HT + ERV. Probability values for effect of 5-HT: P = 0.68, effect of ERV: P = 0.88, and the interaction of 5-HT and ERV: P = 0.54. ( n = 4).
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(A) Representative image of <t>HTR2A</t> protein immunoblot with increasing amounts of total protein (10 μg, 12 μg, 14 μg, 16 μg, 18 μg, 20 μg). (B) Linear regression of pooled total protein extracted from bovine lateral saphenous veins with HTR2A protein expression. (B) Representative image of HTR2A protein immunoblot with increasing amounts of total protein. (C) Representative image of HTR2A protein immunoblot for quantitative protein expression. CON = modified Kreb’s-Henseleit buffer; 5HT = 5 × 10 −7 M 5-HT; ERV = 2.01 × 10 −7 M ERV; 5HT + ERV = 5 × 10 −7 M 5-HT and 2.01 × 10 −7 M ERV. (D) Least squares mean relative HTR2A protein expression ± SEM bovine lateral saphenous veins exposed to control, 5-HT, ERV, or 5-HT + ERV. Probability values for effect of 5-HT: P = 0.68, effect of ERV: P = 0.88, and the interaction of 5-HT and ERV: P = 0.54. ( n = 4).
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Image Search Results


Effect of audiogenic kindling (AK) on messenger RNA (mRNA) expression in the basolateral (A1—D2) and central amygdala (E1—H2) and hippocampus (I1—L2) of serotonin post synaptic receptors 1A ( Htr1a ), 1B ( Htr1b ), 2A ( Htr2a ), and 2C ( Htr2c ) in Wistar and Wistar Audiogenic rats (WAR). These data were analyzed by Two‐way ANOVA followed by Tukey's post‐test or One‐way followed by the Tukey post‐test. # p < 0.05 WARs versus Wistars; *AK versus naive; +AK versus control; @WARs under AK versus Wistars. Box plots display the median (center line), interquartile range (box), and minimum/maximum values (whiskers). All individual data points are shown.

Journal: Journal of Neurochemistry

Article Title: Dysregulated Plasticity in Serotonin, Galanin, and Opioid Systems Contributes to Limbic Seizure Recruitment in Wistar Audiogenic Rat

doi: 10.1111/jnc.70397

Figure Lengend Snippet: Effect of audiogenic kindling (AK) on messenger RNA (mRNA) expression in the basolateral (A1—D2) and central amygdala (E1—H2) and hippocampus (I1—L2) of serotonin post synaptic receptors 1A ( Htr1a ), 1B ( Htr1b ), 2A ( Htr2a ), and 2C ( Htr2c ) in Wistar and Wistar Audiogenic rats (WAR). These data were analyzed by Two‐way ANOVA followed by Tukey's post‐test or One‐way followed by the Tukey post‐test. # p < 0.05 WARs versus Wistars; *AK versus naive; +AK versus control; @WARs under AK versus Wistars. Box plots display the median (center line), interquartile range (box), and minimum/maximum values (whiskers). All individual data points are shown.

Article Snippet: For TaqMan assays, pre‐designed probes were used for Actb (Rn00667869_m1), Htr1a (Rn00561409_s1), Htr1b (Rn01637747_S1), Htr2a (Rn00568473_m1) and Htr2c (Rn00562748_m1), Slc6a4 (Rn00564737_m1), Gal (Rn00583681_m1), Galr1 (Rn02132426_s1), Galr2 (Rn00695901_g1), Tph1 (Rn01476867_m1), Tph2 (Rn00598017_m1), (Applied Biosystems, Beverly, MA, USA).

Techniques: Expressing, Control

(A) Representative image of HTR2A protein immunoblot with increasing amounts of total protein (10 μg, 12 μg, 14 μg, 16 μg, 18 μg, 20 μg). (B) Linear regression of pooled total protein extracted from bovine lateral saphenous veins with HTR2A protein expression. (B) Representative image of HTR2A protein immunoblot with increasing amounts of total protein. (C) Representative image of HTR2A protein immunoblot for quantitative protein expression. CON = modified Kreb’s-Henseleit buffer; 5HT = 5 × 10 −7 M 5-HT; ERV = 2.01 × 10 −7 M ERV; 5HT + ERV = 5 × 10 −7 M 5-HT and 2.01 × 10 −7 M ERV. (D) Least squares mean relative HTR2A protein expression ± SEM bovine lateral saphenous veins exposed to control, 5-HT, ERV, or 5-HT + ERV. Probability values for effect of 5-HT: P = 0.68, effect of ERV: P = 0.88, and the interaction of 5-HT and ERV: P = 0.54. ( n = 4).

Journal: Current Research in Toxicology

Article Title: Association of serotonin and ergot alkaloids on tissue partitioning and contractile response of bovine blood vessels ☆

doi: 10.1016/j.crtox.2025.100272

Figure Lengend Snippet: (A) Representative image of HTR2A protein immunoblot with increasing amounts of total protein (10 μg, 12 μg, 14 μg, 16 μg, 18 μg, 20 μg). (B) Linear regression of pooled total protein extracted from bovine lateral saphenous veins with HTR2A protein expression. (B) Representative image of HTR2A protein immunoblot with increasing amounts of total protein. (C) Representative image of HTR2A protein immunoblot for quantitative protein expression. CON = modified Kreb’s-Henseleit buffer; 5HT = 5 × 10 −7 M 5-HT; ERV = 2.01 × 10 −7 M ERV; 5HT + ERV = 5 × 10 −7 M 5-HT and 2.01 × 10 −7 M ERV. (D) Least squares mean relative HTR2A protein expression ± SEM bovine lateral saphenous veins exposed to control, 5-HT, ERV, or 5-HT + ERV. Probability values for effect of 5-HT: P = 0.68, effect of ERV: P = 0.88, and the interaction of 5-HT and ERV: P = 0.54. ( n = 4).

Article Snippet: Membranes were incubated for overnight at 4 °C with 1:333 mouse HTR2A monoclonal antibody (sc-166775; Santa Cruz Biotechnology Inc.).

Techniques: Western Blot, Expressing, Modification, Control