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CBNK-EVs rapidly impair ZIKV infectivity and protect host cells. (a) Multiplex immunohistochemical analysis of ZIKV infection in neural progenitor cells. Co-staining for Nestin (neural progenitor cells, green) and ZIKV E protein (red) was performed, and double-positive cells were quantified across six random fields, mean ± SD, ∗P < 0.05. Scale bar, 20 μm. (b) KEGG pathway enrichment bubble plot from <t>RNA-seq</t> <t>of</t> <t>HTR-8/Svneo</t> cells treated with CBNK-EVs (3 × 10 11 particles/mL, 10 μl) and ZIKV (MOI = 1) for 1 h. (c) GSEA analysis of transcriptomic data from HTR-8/Svneo cells. (d) Western blot analysis of ZIKV E and ZIKV NS5 protein levels after co-incubation of ZIKV particles with CBNK-EVs for the indicated time periods (0–30 min) prior to infection. (e) The abundance of NK cell-associated proteins in CBNK-EVs. ∗P < 0.05 (one-way ANOVA).
Htr, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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aln htr  (Alnylam Inc)
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CBNK-EVs rapidly impair ZIKV infectivity and protect host cells. (a) Multiplex immunohistochemical analysis of ZIKV infection in neural progenitor cells. Co-staining for Nestin (neural progenitor cells, green) and ZIKV E protein (red) was performed, and double-positive cells were quantified across six random fields, mean ± SD, ∗P < 0.05. Scale bar, 20 μm. (b) KEGG pathway enrichment bubble plot from <t>RNA-seq</t> <t>of</t> <t>HTR-8/Svneo</t> cells treated with CBNK-EVs (3 × 10 11 particles/mL, 10 μl) and ZIKV (MOI = 1) for 1 h. (c) GSEA analysis of transcriptomic data from HTR-8/Svneo cells. (d) Western blot analysis of ZIKV E and ZIKV NS5 protein levels after co-incubation of ZIKV particles with CBNK-EVs for the indicated time periods (0–30 min) prior to infection. (e) The abundance of NK cell-associated proteins in CBNK-EVs. ∗P < 0.05 (one-way ANOVA).
Aln Htr, supplied by Alnylam Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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htr  (Procell Inc)
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Procell Inc htr
CBNK-EVs rapidly impair ZIKV infectivity and protect host cells. (a) Multiplex immunohistochemical analysis of ZIKV infection in neural progenitor cells. Co-staining for Nestin (neural progenitor cells, green) and ZIKV E protein (red) was performed, and double-positive cells were quantified across six random fields, mean ± SD, ∗P < 0.05. Scale bar, 20 μm. (b) KEGG pathway enrichment bubble plot from <t>RNA-seq</t> <t>of</t> <t>HTR-8/Svneo</t> cells treated with CBNK-EVs (3 × 10 11 particles/mL, 10 μl) and ZIKV (MOI = 1) for 1 h. (c) GSEA analysis of transcriptomic data from HTR-8/Svneo cells. (d) Western blot analysis of ZIKV E and ZIKV NS5 protein levels after co-incubation of ZIKV particles with CBNK-EVs for the indicated time periods (0–30 min) prior to infection. (e) The abundance of NK cell-associated proteins in CBNK-EVs. ∗P < 0.05 (one-way ANOVA).
Htr, supplied by Procell Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human extravillous trophoblast cell line htr  (ATCC)
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CBNK-EVs rapidly impair ZIKV infectivity and protect host cells. (a) Multiplex immunohistochemical analysis of ZIKV infection in neural progenitor cells. Co-staining for Nestin (neural progenitor cells, green) and ZIKV E protein (red) was performed, and double-positive cells were quantified across six random fields, mean ± SD, ∗P < 0.05. Scale bar, 20 μm. (b) KEGG pathway enrichment bubble plot from <t>RNA-seq</t> <t>of</t> <t>HTR-8/Svneo</t> cells treated with CBNK-EVs (3 × 10 11 particles/mL, 10 μl) and ZIKV (MOI = 1) for 1 h. (c) GSEA analysis of transcriptomic data from HTR-8/Svneo cells. (d) Western blot analysis of ZIKV E and ZIKV NS5 protein levels after co-incubation of ZIKV particles with CBNK-EVs for the indicated time periods (0–30 min) prior to infection. (e) The abundance of NK cell-associated proteins in CBNK-EVs. ∗P < 0.05 (one-way ANOVA).
Human Extravillous Trophoblast Cell Line Htr, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human extravillous trophoblast cell line htr - by Bioz Stars, 2026-05
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human trophoblast cell line  (ATCC)
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ATCC human trophoblast cell line
CBNK-EVs rapidly impair ZIKV infectivity and protect host cells. (a) Multiplex immunohistochemical analysis of ZIKV infection in neural progenitor cells. Co-staining for Nestin (neural progenitor cells, green) and ZIKV E protein (red) was performed, and double-positive cells were quantified across six random fields, mean ± SD, ∗P < 0.05. Scale bar, 20 μm. (b) KEGG pathway enrichment bubble plot from <t>RNA-seq</t> <t>of</t> <t>HTR-8/Svneo</t> cells treated with CBNK-EVs (3 × 10 11 particles/mL, 10 μl) and ZIKV (MOI = 1) for 1 h. (c) GSEA analysis of transcriptomic data from HTR-8/Svneo cells. (d) Western blot analysis of ZIKV E and ZIKV NS5 protein levels after co-incubation of ZIKV particles with CBNK-EVs for the indicated time periods (0–30 min) prior to infection. (e) The abundance of NK cell-associated proteins in CBNK-EVs. ∗P < 0.05 (one-way ANOVA).
Human Trophoblast Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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htr8 svneo cells  (ATCC)
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ATCC htr8 svneo cells
CBNK-EVs rapidly impair ZIKV infectivity and protect host cells. (a) Multiplex immunohistochemical analysis of ZIKV infection in neural progenitor cells. Co-staining for Nestin (neural progenitor cells, green) and ZIKV E protein (red) was performed, and double-positive cells were quantified across six random fields, mean ± SD, ∗P < 0.05. Scale bar, 20 μm. (b) KEGG pathway enrichment bubble plot from <t>RNA-seq</t> <t>of</t> <t>HTR-8/Svneo</t> cells treated with CBNK-EVs (3 × 10 11 particles/mL, 10 μl) and ZIKV (MOI = 1) for 1 h. (c) GSEA analysis of transcriptomic data from HTR-8/Svneo cells. (d) Western blot analysis of ZIKV E and ZIKV NS5 protein levels after co-incubation of ZIKV particles with CBNK-EVs for the indicated time periods (0–30 min) prior to infection. (e) The abundance of NK cell-associated proteins in CBNK-EVs. ∗P < 0.05 (one-way ANOVA).
Htr8 Svneo Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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htr8 svneo trophoblasts  (ATCC)
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ATCC htr8 svneo trophoblasts
CBNK-EVs rapidly impair ZIKV infectivity and protect host cells. (a) Multiplex immunohistochemical analysis of ZIKV infection in neural progenitor cells. Co-staining for Nestin (neural progenitor cells, green) and ZIKV E protein (red) was performed, and double-positive cells were quantified across six random fields, mean ± SD, ∗P < 0.05. Scale bar, 20 μm. (b) KEGG pathway enrichment bubble plot from <t>RNA-seq</t> <t>of</t> <t>HTR-8/Svneo</t> cells treated with CBNK-EVs (3 × 10 11 particles/mL, 10 μl) and ZIKV (MOI = 1) for 1 h. (c) GSEA analysis of transcriptomic data from HTR-8/Svneo cells. (d) Western blot analysis of ZIKV E and ZIKV NS5 protein levels after co-incubation of ZIKV particles with CBNK-EVs for the indicated time periods (0–30 min) prior to infection. (e) The abundance of NK cell-associated proteins in CBNK-EVs. ∗P < 0.05 (one-way ANOVA).
Htr8 Svneo Trophoblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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CBNK-EVs rapidly impair ZIKV infectivity and protect host cells. (a) Multiplex immunohistochemical analysis of ZIKV infection in neural progenitor cells. Co-staining for Nestin (neural progenitor cells, green) and ZIKV E protein (red) was performed, and double-positive cells were quantified across six random fields, mean ± SD, ∗P < 0.05. Scale bar, 20 μm. (b) KEGG pathway enrichment bubble plot from RNA-seq of HTR-8/Svneo cells treated with CBNK-EVs (3 × 10 11 particles/mL, 10 μl) and ZIKV (MOI = 1) for 1 h. (c) GSEA analysis of transcriptomic data from HTR-8/Svneo cells. (d) Western blot analysis of ZIKV E and ZIKV NS5 protein levels after co-incubation of ZIKV particles with CBNK-EVs for the indicated time periods (0–30 min) prior to infection. (e) The abundance of NK cell-associated proteins in CBNK-EVs. ∗P < 0.05 (one-way ANOVA).

Journal: Bioactive Materials

Article Title: Cord blood natural killer cell-derived extracellular vesicles inhibit Zika virus infectivity through ITGB2/perforin-mediated envelope disruption in vitro and in vivo

doi: 10.1016/j.bioactmat.2026.01.030

Figure Lengend Snippet: CBNK-EVs rapidly impair ZIKV infectivity and protect host cells. (a) Multiplex immunohistochemical analysis of ZIKV infection in neural progenitor cells. Co-staining for Nestin (neural progenitor cells, green) and ZIKV E protein (red) was performed, and double-positive cells were quantified across six random fields, mean ± SD, ∗P < 0.05. Scale bar, 20 μm. (b) KEGG pathway enrichment bubble plot from RNA-seq of HTR-8/Svneo cells treated with CBNK-EVs (3 × 10 11 particles/mL, 10 μl) and ZIKV (MOI = 1) for 1 h. (c) GSEA analysis of transcriptomic data from HTR-8/Svneo cells. (d) Western blot analysis of ZIKV E and ZIKV NS5 protein levels after co-incubation of ZIKV particles with CBNK-EVs for the indicated time periods (0–30 min) prior to infection. (e) The abundance of NK cell-associated proteins in CBNK-EVs. ∗P < 0.05 (one-way ANOVA).

Article Snippet: Vero E6, BHK21, 293T and HTR-8/Svneo cells were purchased from ATCC.

Techniques: Infection, Multiplex Assay, Immunohistochemical staining, Staining, RNA Sequencing, Western Blot, Incubation