Journal: iScience

Article Title: Triple-armed oncolytic HSV enhances antitumor immunity by remodeling the tumor microenvironment in subcutaneous murine CRC

doi: 10.1016/j.isci.2026.115883

Figure Lengend Snippet: oHSV-1 exhibits the characteristics of a standard one-step growth curve and can efficiently replicate and express exogenous genes (A) The results of virus titer determination of HSV-1/WT, RG2001m, RG2002m, RG2005m, RG2011m, and RG2012m in Vero cells 48 h after infection. (B) Vero cells were infected with oHSV-1 at a multiplicity of infection (MOI) = 1, and the virus titers were determined at 0, 8, 16, 24, 32, 40, and 48 h, respectively, and a growth curve was drawn. (C) The results of virus titer determination of oHSV-1 in colon cancer cells (CT26, HCT116, and HT29) 48 h after infection. (D–F) The growth curves of oHSV-1 in colon cancer cells (CT26, HCT116, and HT29). (G) At MOI = 10, IL-15 expression levels in cell supernatants of HSV-1/WT, RG2001m, RG2005m, and RG2012m at 24, 48, and 72 h post-infection (mouse IL-15 ELISA Kit, elabscience). (H) At MOI = 10, IL-12 expression levels in cell supernatants of HSV-1/WT, RG2002m, RG2005m, and RG2012m at 24, 48, and 72 h post-infection (mouse IL-12 ELISA Kit, elabscience). (I) At MOI = 10, chemokine CCL5 expression levels in cell supernatants of HSV-1/WT, RG2011m, and RG2012m at 24, 48, and 72 h post-infection (mouse CCL5 ELISA Kit, elabscience). (Data are presented as mean ± SD. Comparisons between datasets were performed using one-way ANOVA and Student’s t test, with p values calculated [ n = 3]) ( p > 0.05, ns; p < 0.05, ∗; p < 0.01, ∗ ∗; p < 0.001, ∗ ∗ ∗).

Article Snippet: CT26, HTC116, and HT29 cells were purchased from Wuhan Procell Life Science & Technology Co., Ltd., China (Cat. No. CL-0071; Cat. No. CL-0096; Cat. No. CL-0118).

Techniques: Virus, Infection, Expressing, Enzyme-linked Immunosorbent Assay

Journal: iScience

Article Title: Triple-armed oncolytic HSV enhances antitumor immunity by remodeling the tumor microenvironment in subcutaneous murine CRC

doi: 10.1016/j.isci.2026.115883

Figure Lengend Snippet: Killing effect of recombinant oHSV-1 on colon cancer cells (A–C) CT26 (A), HCT116 (B), and HT29 (C) colon cancer cells were infected with recombinant oncolytic virus at multiplicity of infection (MOI) of 0.5, 1, and 5, respectively. The cell killing rate was detected by cell counting kit-8 (CCK8) assay 48 h after infection. The results showed that the killing effect of the virus on the three colon cancer cells was enhanced in an MOI - dependent manner. (D–F) CT26 (D), HCT116 (E), and HT29 (F) cells were infected with recombinant oncolytic virus at MOI = 1. The killing rate was measured by CCK8 assay at 24, 48, and 72 h after infection, suggesting that the killing effect of the virus on colon cancer cells was significantly enhanced with the prolongation of action time. (The values are presented as mean ± SD. The datasets were compared via one-way ANOVA. n = 3) ( p > 0.05, ns; p < 0.05, ∗; p < 0.01, ∗ ∗; p < 0.001, ∗ ∗ ∗.).

Article Snippet: CT26, HTC116, and HT29 cells were purchased from Wuhan Procell Life Science & Technology Co., Ltd., China (Cat. No. CL-0071; Cat. No. CL-0096; Cat. No. CL-0118).

Techniques: Recombinant, Infection, Virus, Cell Counting, CCK-8 Assay

Journal: iScience

Article Title: Triple-armed oncolytic HSV enhances antitumor immunity by remodeling the tumor microenvironment in subcutaneous murine CRC

doi: 10.1016/j.isci.2026.115883

Figure Lengend Snippet: oHSV-1 exhibits the characteristics of a standard one-step growth curve and can efficiently replicate and express exogenous genes (A) The results of virus titer determination of HSV-1/WT, RG2001m, RG2002m, RG2005m, RG2011m, and RG2012m in Vero cells 48 h after infection. (B) Vero cells were infected with oHSV-1 at a multiplicity of infection (MOI) = 1, and the virus titers were determined at 0, 8, 16, 24, 32, 40, and 48 h, respectively, and a growth curve was drawn. (C) The results of virus titer determination of oHSV-1 in colon cancer cells (CT26, HCT116, and HT29) 48 h after infection. (D–F) The growth curves of oHSV-1 in colon cancer cells (CT26, HCT116, and HT29). (G) At MOI = 10, IL-15 expression levels in cell supernatants of HSV-1/WT, RG2001m, RG2005m, and RG2012m at 24, 48, and 72 h post-infection (mouse IL-15 ELISA Kit, elabscience). (H) At MOI = 10, IL-12 expression levels in cell supernatants of HSV-1/WT, RG2002m, RG2005m, and RG2012m at 24, 48, and 72 h post-infection (mouse IL-12 ELISA Kit, elabscience). (I) At MOI = 10, chemokine CCL5 expression levels in cell supernatants of HSV-1/WT, RG2011m, and RG2012m at 24, 48, and 72 h post-infection (mouse CCL5 ELISA Kit, elabscience). (Data are presented as mean ± SD. Comparisons between datasets were performed using one-way ANOVA and Student’s t test, with p values calculated [ n = 3]) ( p > 0.05, ns; p < 0.05, ∗; p < 0.01, ∗ ∗; p < 0.001, ∗ ∗ ∗).

Article Snippet: HT29 , Wuhan Procell Life Science & Technology Co., Ltd., China , Cat. No. CL-0118; RRID: CVCL_0320.

Techniques: Virus, Infection, Expressing, Enzyme-linked Immunosorbent Assay

Journal: iScience

Article Title: Triple-armed oncolytic HSV enhances antitumor immunity by remodeling the tumor microenvironment in subcutaneous murine CRC

doi: 10.1016/j.isci.2026.115883

Figure Lengend Snippet: Killing effect of recombinant oHSV-1 on colon cancer cells (A–C) CT26 (A), HCT116 (B), and HT29 (C) colon cancer cells were infected with recombinant oncolytic virus at multiplicity of infection (MOI) of 0.5, 1, and 5, respectively. The cell killing rate was detected by cell counting kit-8 (CCK8) assay 48 h after infection. The results showed that the killing effect of the virus on the three colon cancer cells was enhanced in an MOI - dependent manner. (D–F) CT26 (D), HCT116 (E), and HT29 (F) cells were infected with recombinant oncolytic virus at MOI = 1. The killing rate was measured by CCK8 assay at 24, 48, and 72 h after infection, suggesting that the killing effect of the virus on colon cancer cells was significantly enhanced with the prolongation of action time. (The values are presented as mean ± SD. The datasets were compared via one-way ANOVA. n = 3) ( p > 0.05, ns; p < 0.05, ∗; p < 0.01, ∗ ∗; p < 0.001, ∗ ∗ ∗.).

Article Snippet: HT29 , Wuhan Procell Life Science & Technology Co., Ltd., China , Cat. No. CL-0118; RRID: CVCL_0320.

Techniques: Recombinant, Infection, Virus, Cell Counting, CCK-8 Assay