Review





Similar Products

pmd201t hsvd elvd  (TaKaRa)
99
TaKaRa pmd201t hsvd elvd
Schematic representation of viroid dimerization and subsequent assembly in a binary vector. a Two different pairs of primers are used to generate distal Bsa I recognition sites (magenta) in such a way that compatible ends for assembly can be obtained. b In a single reaction of simultaneous restriction and ligation the two viroid monomers (blue) are ligated between them and to a binary vector with compatible cohesive ends (orange). c Specifically, the viroid dimer is inserted into an expression cassette that contains and a duplicated 35S, a constitutive promoter for plant expression and a PoPit terminator. Additionally, this binary vector has a T7 promoter for in vitro transcription. The dimeric viroid cDNA sequence replaces a lethal gene ccdB, thus guaranteeing an optimal efficiency of the reaction. d Detail of the generation of the receptor–vector starting from <t>pMD201t</t> construct)
Pmd201t Hsvd Elvd, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hsvd/pmc06670230-65-11-21?v=TaKaRa
Average 99 stars, based on 1 article reviews
pmd201t hsvd elvd - by Bioz Stars, 2026-07
99/100 stars
  Buy from Supplier
hsvd  (Shilpa Medicare)
90
Shilpa Medicare hsvd
Schematic representation of viroid dimerization and subsequent assembly in a binary vector. a Two different pairs of primers are used to generate distal Bsa I recognition sites (magenta) in such a way that compatible ends for assembly can be obtained. b In a single reaction of simultaneous restriction and ligation the two viroid monomers (blue) are ligated between them and to a binary vector with compatible cohesive ends (orange). c Specifically, the viroid dimer is inserted into an expression cassette that contains and a duplicated 35S, a constitutive promoter for plant expression and a PoPit terminator. Additionally, this binary vector has a T7 promoter for in vitro transcription. The dimeric viroid cDNA sequence replaces a lethal gene ccdB, thus guaranteeing an optimal efficiency of the reaction. d Detail of the generation of the receptor–vector starting from <t>pMD201t</t> construct)
Hsvd, supplied by Shilpa Medicare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hsvd/pm38698140-24-24-32?v=Shilpa+Medicare
Average 90 stars, based on 1 article reviews
hsvd - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
hsvd infection  (Shilpa Medicare)
90
Shilpa Medicare hsvd infection
Schematic representation of viroid dimerization and subsequent assembly in a binary vector. a Two different pairs of primers are used to generate distal Bsa I recognition sites (magenta) in such a way that compatible ends for assembly can be obtained. b In a single reaction of simultaneous restriction and ligation the two viroid monomers (blue) are ligated between them and to a binary vector with compatible cohesive ends (orange). c Specifically, the viroid dimer is inserted into an expression cassette that contains and a duplicated 35S, a constitutive promoter for plant expression and a PoPit terminator. Additionally, this binary vector has a T7 promoter for in vitro transcription. The dimeric viroid cDNA sequence replaces a lethal gene ccdB, thus guaranteeing an optimal efficiency of the reaction. d Detail of the generation of the receptor–vector starting from <t>pMD201t</t> construct)
Hsvd Infection, supplied by Shilpa Medicare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hsvd/pm38698140-104-1-13?v=Shilpa+Medicare
Average 90 stars, based on 1 article reviews
hsvd infection - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
hsvd isolates  (Shilpa Medicare)
90
Shilpa Medicare hsvd isolates
Schematic representation of viroid dimerization and subsequent assembly in a binary vector. a Two different pairs of primers are used to generate distal Bsa I recognition sites (magenta) in such a way that compatible ends for assembly can be obtained. b In a single reaction of simultaneous restriction and ligation the two viroid monomers (blue) are ligated between them and to a binary vector with compatible cohesive ends (orange). c Specifically, the viroid dimer is inserted into an expression cassette that contains and a duplicated 35S, a constitutive promoter for plant expression and a PoPit terminator. Additionally, this binary vector has a T7 promoter for in vitro transcription. The dimeric viroid cDNA sequence replaces a lethal gene ccdB, thus guaranteeing an optimal efficiency of the reaction. d Detail of the generation of the receptor–vector starting from <t>pMD201t</t> construct)
Hsvd Isolates, supplied by Shilpa Medicare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hsvd/pm38698140-77-5-17?v=Shilpa+Medicare
Average 90 stars, based on 1 article reviews
hsvd isolates - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
hsvd  (Kirin Brewery Company)
90
Kirin Brewery Company hsvd
Schematic representation of viroid dimerization and subsequent assembly in a binary vector. a Two different pairs of primers are used to generate distal Bsa I recognition sites (magenta) in such a way that compatible ends for assembly can be obtained. b In a single reaction of simultaneous restriction and ligation the two viroid monomers (blue) are ligated between them and to a binary vector with compatible cohesive ends (orange). c Specifically, the viroid dimer is inserted into an expression cassette that contains and a duplicated 35S, a constitutive promoter for plant expression and a PoPit terminator. Additionally, this binary vector has a T7 promoter for in vitro transcription. The dimeric viroid cDNA sequence replaces a lethal gene ccdB, thus guaranteeing an optimal efficiency of the reaction. d Detail of the generation of the receptor–vector starting from <t>pMD201t</t> construct)
Hsvd, supplied by Kirin Brewery Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hsvd/pm36992390-236-8-11?v=Kirin+Brewery+Company
Average 90 stars, based on 1 article reviews
hsvd - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
hsvd bayesian 0  (Bruker Corporation)
90
Bruker Corporation hsvd bayesian 0
Schematic representation of viroid dimerization and subsequent assembly in a binary vector. a Two different pairs of primers are used to generate distal Bsa I recognition sites (magenta) in such a way that compatible ends for assembly can be obtained. b In a single reaction of simultaneous restriction and ligation the two viroid monomers (blue) are ligated between them and to a binary vector with compatible cohesive ends (orange). c Specifically, the viroid dimer is inserted into an expression cassette that contains and a duplicated 35S, a constitutive promoter for plant expression and a PoPit terminator. Additionally, this binary vector has a T7 promoter for in vitro transcription. The dimeric viroid cDNA sequence replaces a lethal gene ccdB, thus guaranteeing an optimal efficiency of the reaction. d Detail of the generation of the receptor–vector starting from <t>pMD201t</t> construct)
Hsvd Bayesian 0, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hsvd/pm29127944-395-0-3?v=Bruker+Corporation
Average 90 stars, based on 1 article reviews
hsvd bayesian 0 - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
hsvd  (Thermo Fisher)
90
Thermo Fisher hsvd
Schematic representation of viroid dimerization and subsequent assembly in a binary vector. a Two different pairs of primers are used to generate distal Bsa I recognition sites (magenta) in such a way that compatible ends for assembly can be obtained. b In a single reaction of simultaneous restriction and ligation the two viroid monomers (blue) are ligated between them and to a binary vector with compatible cohesive ends (orange). c Specifically, the viroid dimer is inserted into an expression cassette that contains and a duplicated 35S, a constitutive promoter for plant expression and a PoPit terminator. Additionally, this binary vector has a T7 promoter for in vitro transcription. The dimeric viroid cDNA sequence replaces a lethal gene ccdB, thus guaranteeing an optimal efficiency of the reaction. d Detail of the generation of the receptor–vector starting from <t>pMD201t</t> construct)
Hsvd, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hsvd/pm33752714-205-5-14?v=Thermo+Fisher
Average 90 stars, based on 1 article reviews
hsvd - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
hsvd  (Illumina Inc)
90
Illumina Inc hsvd
Schematic representation of viroid dimerization and subsequent assembly in a binary vector. a Two different pairs of primers are used to generate distal Bsa I recognition sites (magenta) in such a way that compatible ends for assembly can be obtained. b In a single reaction of simultaneous restriction and ligation the two viroid monomers (blue) are ligated between them and to a binary vector with compatible cohesive ends (orange). c Specifically, the viroid dimer is inserted into an expression cassette that contains and a duplicated 35S, a constitutive promoter for plant expression and a PoPit terminator. Additionally, this binary vector has a T7 promoter for in vitro transcription. The dimeric viroid cDNA sequence replaces a lethal gene ccdB, thus guaranteeing an optimal efficiency of the reaction. d Detail of the generation of the receptor–vector starting from <t>pMD201t</t> construct)
Hsvd, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hsvd/pm33752714-205-23-9?v=Illumina+Inc
Average 90 stars, based on 1 article reviews
hsvd - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
hsvd algorithm 22  (MathWorks Inc)
90
MathWorks Inc hsvd algorithm 22
Schematic representation of viroid dimerization and subsequent assembly in a binary vector. a Two different pairs of primers are used to generate distal Bsa I recognition sites (magenta) in such a way that compatible ends for assembly can be obtained. b In a single reaction of simultaneous restriction and ligation the two viroid monomers (blue) are ligated between them and to a binary vector with compatible cohesive ends (orange). c Specifically, the viroid dimer is inserted into an expression cassette that contains and a duplicated 35S, a constitutive promoter for plant expression and a PoPit terminator. Additionally, this binary vector has a T7 promoter for in vitro transcription. The dimeric viroid cDNA sequence replaces a lethal gene ccdB, thus guaranteeing an optimal efficiency of the reaction. d Detail of the generation of the receptor–vector starting from <t>pMD201t</t> construct)
Hsvd Algorithm 22, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hsvd/pmc05773372-85-24-28?v=MathWorks+Inc
Average 90 stars, based on 1 article reviews
hsvd algorithm 22 - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier

Image Search Results


Schematic representation of viroid dimerization and subsequent assembly in a binary vector. a Two different pairs of primers are used to generate distal Bsa I recognition sites (magenta) in such a way that compatible ends for assembly can be obtained. b In a single reaction of simultaneous restriction and ligation the two viroid monomers (blue) are ligated between them and to a binary vector with compatible cohesive ends (orange). c Specifically, the viroid dimer is inserted into an expression cassette that contains and a duplicated 35S, a constitutive promoter for plant expression and a PoPit terminator. Additionally, this binary vector has a T7 promoter for in vitro transcription. The dimeric viroid cDNA sequence replaces a lethal gene ccdB, thus guaranteeing an optimal efficiency of the reaction. d Detail of the generation of the receptor–vector starting from pMD201t construct)

Journal: Plant Methods

Article Title: Highly efficient construction of infectious viroid-derived clones

doi: 10.1186/s13007-019-0470-4

Figure Lengend Snippet: Schematic representation of viroid dimerization and subsequent assembly in a binary vector. a Two different pairs of primers are used to generate distal Bsa I recognition sites (magenta) in such a way that compatible ends for assembly can be obtained. b In a single reaction of simultaneous restriction and ligation the two viroid monomers (blue) are ligated between them and to a binary vector with compatible cohesive ends (orange). c Specifically, the viroid dimer is inserted into an expression cassette that contains and a duplicated 35S, a constitutive promoter for plant expression and a PoPit terminator. Additionally, this binary vector has a T7 promoter for in vitro transcription. The dimeric viroid cDNA sequence replaces a lethal gene ccdB, thus guaranteeing an optimal efficiency of the reaction. d Detail of the generation of the receptor–vector starting from pMD201t construct)

Article Snippet: Viroid transcripts were generated by transcription of 400 ng of linearized pMD201t HSVd/ELVd (Digested with Eco RI) with T7 RNA polymerase (Takara, Kusatsu, Japan) for 3 h according to manufacturer instructions.

Techniques: Plasmid Preparation, Ligation, Expressing, In Vitro, Sequencing, Construct

Workflow proposed to obtain infectious clones of a viroid. Viroid sequence can be amplified from infected tissue by RT-PCR or from a DNA source by PCR. If the viroid sequence does not contain a bsa I recognition site, the viroidal cDNA can be directly assembled into the binary vector (pMD201t), replacing a lethal gene, in a simultaneous bsa I restriction and ligation. Conversely, if there the viroid contains a bsa I recognition site, it can be cloned using another IIs enzyme, such as Bsm BI. Once digested and purified, the viroid cDNA is dimerized by ligation to a previously digested pMD201t. The dimeric viroidal cDNA cloned into pMD201t (pMD201t-viroid) can be used to generate the infectious RNA transcript in vitro, using T7 RNA polymerase onto a linearized plasmid (digested with Eco RI). Additionally, the pMD201t-viroid can be transformed into Agrobacterium tumefaciens for transient plant transformation and subsequent production of infective RNA in vivo

Journal: Plant Methods

Article Title: Highly efficient construction of infectious viroid-derived clones

doi: 10.1186/s13007-019-0470-4

Figure Lengend Snippet: Workflow proposed to obtain infectious clones of a viroid. Viroid sequence can be amplified from infected tissue by RT-PCR or from a DNA source by PCR. If the viroid sequence does not contain a bsa I recognition site, the viroidal cDNA can be directly assembled into the binary vector (pMD201t), replacing a lethal gene, in a simultaneous bsa I restriction and ligation. Conversely, if there the viroid contains a bsa I recognition site, it can be cloned using another IIs enzyme, such as Bsm BI. Once digested and purified, the viroid cDNA is dimerized by ligation to a previously digested pMD201t. The dimeric viroidal cDNA cloned into pMD201t (pMD201t-viroid) can be used to generate the infectious RNA transcript in vitro, using T7 RNA polymerase onto a linearized plasmid (digested with Eco RI). Additionally, the pMD201t-viroid can be transformed into Agrobacterium tumefaciens for transient plant transformation and subsequent production of infective RNA in vivo

Article Snippet: Viroid transcripts were generated by transcription of 400 ng of linearized pMD201t HSVd/ELVd (Digested with Eco RI) with T7 RNA polymerase (Takara, Kusatsu, Japan) for 3 h according to manufacturer instructions.

Techniques: Clone Assay, Sequencing, Amplification, Infection, Reverse Transcription Polymerase Chain Reaction, Plasmid Preparation, Ligation, Purification, In Vitro, Transformation Assay, In Vivo