Journal: Scientific Reports

Article Title: Collagen-specific molecular chaperone Hsp47 in inguinal white adipose tissue promotes high-fat diet-induced inflammatory gene expression in male mice

doi: 10.1038/s41598-026-45003-4

Figure Lengend Snippet: High-fat diet induces Hsp47 expression in adipose tissue. (a ) Immunoblot of Hsp47 and GAPDH in epiWAT (epididymal white adipose tissue) and ingWAT (inguinal white adipose tissue) from mice fed a normal diet (ND) or high-fat diet (HFD) for 12 weeks. ( b ) Quantification of Hsp47 protein (normalized to GAPDH) in epiWAT and ingWAT. ( c ) Quantification of Hsp47 mRNA (normalized to Gapdh) in epiWAT and ingWAT. ( d ) Immunoblots confirming adipose-specific Hsp47 deletion. Samples: ND flox (ND-fed Hsp47 flox/flox), ND aKO (ND-fed Adipoq-Cre; Hsp47 flox/flox), HFD flox (HFD-fed Hsp47 flox/flox), and HFD aKO (HFD-fed Adipoq-Cre; Hsp47 flox/flox). Blots shown for Hsp47, the ER chaperone Grp94, and loading controls (β-actin) in epiWAT and lung. ( e ) Quantification of Hsp47 protein level in epiWAT, ( f ) Quantification of Hsp47 protein level in lung tissue, ( g ) Quantification of Grp94 (ER stress marker) protein level in epiWAT. Data are presented as mean ± SD. Group comparisons were assessed by two-tailed t-tests. Variance equality was evaluated using the Fligner–Killeen test; when group variances were not significantly different ( P > 0.05), Student’s t-test was applied, otherwise Welch’s t-test was used. *, **, *** indicate P < 0.05, 0.01, 0.001, respectively; n.s., not significant. Exact P values are provided in Supplementary Table .

Article Snippet: Adipose tissue-specific Hsp47 conditional-knockout (aKO) mice were generated by crossing Hsp47 floxed mice and Adipoq-Cre (adipose tissue-specific promoter) transgenic animals (B6;FVB-Tg( Adipoq -Cre)1Evdr/J, 010803, The Jackson Laboratory) .

Techniques: Expressing, Western Blot, Marker, Two Tailed Test

Journal: Scientific Reports

Article Title: Collagen-specific molecular chaperone Hsp47 in inguinal white adipose tissue promotes high-fat diet-induced inflammatory gene expression in male mice

doi: 10.1038/s41598-026-45003-4

Figure Lengend Snippet: Adipocyte Hsp47 drives collagen deposition and adipocyte hypertrophy in inguinal white adipose tissue under a high-fat diet. (a) Representative Sirius Red–stained sections of epididymal (epiWAT) and inguinal (ingWAT) white adipose tissue from Hsp47 flox/flox (flox) and adipocyte-specific Hsp47 knockout (aKO; Adipoq-Cre; Hsp47 flox/flox) mice after 12 weeks on a normal diet (ND) or high-fat diet (HFD). Scale bars, 100 μm.

Article Snippet: Adipose tissue-specific Hsp47 conditional-knockout (aKO) mice were generated by crossing Hsp47 floxed mice and Adipoq-Cre (adipose tissue-specific promoter) transgenic animals (B6;FVB-Tg( Adipoq -Cre)1Evdr/J, 010803, The Jackson Laboratory) .

Techniques: Staining, Knock-Out

Journal: Scientific Reports

Article Title: Collagen-specific molecular chaperone Hsp47 in inguinal white adipose tissue promotes high-fat diet-induced inflammatory gene expression in male mice

doi: 10.1038/s41598-026-45003-4

Figure Lengend Snippet: Adipose Hsp47 knockout attenuates HFD-induced inflammatory and fibrotic gene expression in ingWAT. Relative mRNA expression of Col1a1, Col3a1, Col6a1, Fn1 (fibronectin), Il6 (interleukin-6), Tnfa (tumor necrosis factor-α), and Ccl2 (monocyte chemoattractant protein-1) in epididymal (epiWAT) and inguinal (ingWAT) white adipose tissue from mice fed a normal diet (ND) or high-fat diet (HFD) for 12 weeks, measured by qPCR (normalized to Gapdh). Data are presented as mean ± SD. Group comparisons were assessed by two-tailed t-tests. Variance equality was evaluated using the Fligner–Killeen test; when group variances were not significantly different ( P > 0.05), Student’s t-test was applied, otherwise Welch’s t-test was used. *, **, *** indicate P < 0.05, 0.01, 0.001, respectively; n.s., not significant. Exact P values are provided in Supplementary Table .

Article Snippet: Adipose tissue-specific Hsp47 conditional-knockout (aKO) mice were generated by crossing Hsp47 floxed mice and Adipoq-Cre (adipose tissue-specific promoter) transgenic animals (B6;FVB-Tg( Adipoq -Cre)1Evdr/J, 010803, The Jackson Laboratory) .

Techniques: Knock-Out, Gene Expression, Expressing, Two Tailed Test

Journal: Scientific Reports

Article Title: Collagen-specific molecular chaperone Hsp47 in inguinal white adipose tissue promotes high-fat diet-induced inflammatory gene expression in male mice

doi: 10.1038/s41598-026-45003-4

Figure Lengend Snippet: Adipose-specific Hsp47 knockout mitigates HFD-associated systemic and renal stress markers. Serum analytes: glucose, HDL-C (high-density lipoprotein cholesterol), inorganic phosphate (IP), calcium (Ca), and albumin; Urine analytes: urea nitrogen (U-UN), creatinine (U-CRE), uric acid (U-UA), potassium (U-K), and inorganic phosphate (U-IP). Data are presented as mean ± SD. Group comparisons were assessed by two-tailed t-tests. Variance equality was evaluated using the Fligner–Killeen test; when group variances were not significantly different ( P > 0.05), Student’s t-test was applied, otherwise Welch’s t-test was used. *, **, *** indicate P < 0.05, 0.01, 0.001, respectively; n.s., not significant. Exact P values are provided in Supplementary Table .

Article Snippet: Adipose tissue-specific Hsp47 conditional-knockout (aKO) mice were generated by crossing Hsp47 floxed mice and Adipoq-Cre (adipose tissue-specific promoter) transgenic animals (B6;FVB-Tg( Adipoq -Cre)1Evdr/J, 010803, The Jackson Laboratory) .

Techniques: Knock-Out, Two Tailed Test

Journal: Bioactive Materials

Article Title: Mn 3 O 4 -potentiated bifunctional hydrogel for mild temperature-controlled tumor ablation and osteogenesis

doi: 10.1016/j.bioactmat.2025.09.037

Figure Lengend Snippet: Osteogenesis of the GM/OD/Mn 3 O 4 hydrogel with mild-temperature photothermal stimulation in vitro . (a) Simplified schematic of cell culture and osteogenic differentiation under NIR laser irradiation. (b) Live/dead and (c) morphology fluorescence staining images of BMSCs incubated with different hydrogels for 4 days (scale bar = 100 μm). Qualitative staining and quantitative analysis of (d, e) ALP and (f, g) ARS for BMSCs in different groups. Graph of gene expression changes in (h) HSP70 and (i) HSP47 in BMSCs. (j–m) Relative expression levels of osteogenic-related marker genes in BMSCs treated by different groups. (n) WB analysis of the expressions of HSPs and osteogenic proteins in BMSCs. ACTIN served as an internal control for equal loading. Data represent means ± standard deviations (n = 5). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

Article Snippet: Protein expression analysis: WB was conducted on day 7 to detect HSP70 and HSP47 protein levels, and on day 14 to assess COLІ, BMP-2, RUNX2, and OCN (antibodies sourced from Abmart).

Techniques: In Vitro, Cell Culture, Irradiation, Fluorescence, Staining, Incubation, Gene Expression, Expressing, Marker, Control