Journal: Scientific Reports

Article Title: Comparing and combining xevinapant with ATR and PARP inhibition for the radiosensitization of HPV-negative HNSCC cells

doi: 10.1038/s41598-026-38550-3

Figure Lengend Snippet: Cell death induction. Cells were seeded, after 3 h treated with substances and after an additional 2 h irradiated as indicated. 48 h later cells were harvested, stained for annexin V and DAPI and analyzed using flow cytometry. ( A ) Examples and gating. Events in the lower right rectangle were counted as early apoptosis and in the upper right rectangle as lytic cell death. Shown are HSC4 cells. ( B ) Quantification of early apoptosis and ( C ) quantification of lytic cell death. Significant differences to the respective irradiated or non-irradiated DMSO control are indicated with *, ** and *** indicating p < 0.05, p < 0.01 and p < 0.001, respectively (paired, two-tailed Student’s t-test). Asterisks in brackets indicate significant differences between irradiated and non-irradiated DMSO controls. Results are based on at least 4 individual experiments per cell line. Graphs display mean and standard deviation.

Article Snippet: HSC4 and SAS are also commercially available (AcceGen -#ABC-TC0420 & #ABL-TC0611, as of 05.01.2026).

Techniques: Irradiation, Staining, Flow Cytometry, Control, Two Tailed Test, Standard Deviation

Journal: International Journal of Oral Science

Article Title: Porphyromonas gingivalis potentiates stem-like properties of oral squamous cell carcinoma by modulating SCD1-dependent lipid synthesis via NOD1/KLF5 axis

doi: 10.1038/s41368-024-00342-8

Figure Lengend Snippet: The correlation of P. gingivalis abundance and cancer stem cell markers expression in OSCC tissues. a Representative IHC images of ALDH1, BMI1, NANOG, and SOX2 in OSCC samples. Scale bar: 50 μm. b Quantitative analysis of ALDH1, BMI1, NANOG, and SOX2 in OSCC samples. n = 30. Data are presented as mean ± SD. * P < 0.05, ** P < 0.01. c The correlations between P. gingivalis and the expression of ALDH1, BMI1, NANOG, and SOX2 were analyzed with Pearson correlation analysis. n = 20

Article Snippet: The OSCC cell lines HSC-4 and SCC-9 were originally derived from a patient with tongue cancer and respectively obtained from the Japanese Collection of Research Bioresources Cell Bank (JCRB; Shinjuku, Japan) and American Type Culture Collection (ATCC, Manassas, USA).

Techniques: Expressing

Journal: International Journal of Oral Science

Article Title: Porphyromonas gingivalis potentiates stem-like properties of oral squamous cell carcinoma by modulating SCD1-dependent lipid synthesis via NOD1/KLF5 axis

doi: 10.1038/s41368-024-00342-8

Figure Lengend Snippet: Persistent exposure to P. gingivalis promoted OSCC cells to acquire stem-like features. a Colony formation showed P. gingivalis increased the number of cell clones. b Sphere formation showed P. gingivalis increased the diameter of tumorspheres. Scale bar: 100 μm. c , d Western blot and quantification showed the upregulation of stemness signature proteins BMI1, NANOG, and SOX2 compared to the control group. β-actin was used as a housekeeping gene. e Flow cytometry showed P. gingivalis increased the proportion of ALDH1 + subpopulation of HSC-4 and SCC-9 cells. n = 3. Data are presented as mean ± SD. ns: P > 0.05, * P < 0.05, ** P < 0.01

Article Snippet: The OSCC cell lines HSC-4 and SCC-9 were originally derived from a patient with tongue cancer and respectively obtained from the Japanese Collection of Research Bioresources Cell Bank (JCRB; Shinjuku, Japan) and American Type Culture Collection (ATCC, Manassas, USA).

Techniques: Clone Assay, Western Blot, Control, Flow Cytometry

Journal: International Journal of Oral Science

Article Title: Porphyromonas gingivalis potentiates stem-like properties of oral squamous cell carcinoma by modulating SCD1-dependent lipid synthesis via NOD1/KLF5 axis

doi: 10.1038/s41368-024-00342-8

Figure Lengend Snippet: P. gingivalis induced OSCC cells to acquire stem-like features by regulating lipid synthesis. a Volcano plot of differentially expressed genes (DEGs) between HSC-4 cells treated with and without P. gingivalis . b Top twenty most enriched GO (BP) pathways enrichment analysis for differential genes were visible. c Nile red staining showed Triacsin C abolished P. gingivalis -induced lipid droplets accumulation. Scale bar: 20 μm. d Sphere formation showed Triacsin C decreased P. gingivalis -induced the diameter of tumorspheres. Scale bar: 100 μm. e Western blot showed Triacsin C significantly suppressed P. gingivalis -induced upregulation of BMI1, NANOG, and SOX2. β-actin was used as a housekeeping gene. n = 3

Article Snippet: The OSCC cell lines HSC-4 and SCC-9 were originally derived from a patient with tongue cancer and respectively obtained from the Japanese Collection of Research Bioresources Cell Bank (JCRB; Shinjuku, Japan) and American Type Culture Collection (ATCC, Manassas, USA).

Techniques: Staining, Western Blot

Journal: International Journal of Oral Science

Article Title: Porphyromonas gingivalis potentiates stem-like properties of oral squamous cell carcinoma by modulating SCD1-dependent lipid synthesis via NOD1/KLF5 axis

doi: 10.1038/s41368-024-00342-8

Figure Lengend Snippet: P. gingivalis induced OSCC cells to acquire stem-like features by modulating SCD1-mediated lipid synthesis. a , b Colony formation and quantification showed knockdown of SCD1 decreased P. gingivalis -induced the number of cell clones. c , d Sphere formation and quantification showed knockdown of SCD1 decreased P. gingivalis -induced the number and diameter of tumorspheres. Scale bar: 100 μm. n = 3. Data are presented as mean ± SD. ** P < 0.01

Article Snippet: The OSCC cell lines HSC-4 and SCC-9 were originally derived from a patient with tongue cancer and respectively obtained from the Japanese Collection of Research Bioresources Cell Bank (JCRB; Shinjuku, Japan) and American Type Culture Collection (ATCC, Manassas, USA).

Techniques: Knockdown, Clone Assay

Journal: International Journal of Oral Science

Article Title: Porphyromonas gingivalis potentiates stem-like properties of oral squamous cell carcinoma by modulating SCD1-dependent lipid synthesis via NOD1/KLF5 axis

doi: 10.1038/s41368-024-00342-8

Figure Lengend Snippet: P. gingivalis induced OSCC cells to express stemness markers by modulating SCD1-mediated lipid synthesis. a , b Western blot and quantification showed knockdown of SCD1 suppressed P. gingivalis -induced upregulation of BMI1, NANOG, and SOX2. β-actin was used as a housekeeping gene. c , d Flow cytometry and quantification showed knockdown of SCD1 decreased P. gingivalis- increased the proportion of ALDH1 + subpopulation of OSCC cells. n = 3. Data are presented as mean ± SD. * P < 0.05, ** P < 0.01

Article Snippet: The OSCC cell lines HSC-4 and SCC-9 were originally derived from a patient with tongue cancer and respectively obtained from the Japanese Collection of Research Bioresources Cell Bank (JCRB; Shinjuku, Japan) and American Type Culture Collection (ATCC, Manassas, USA).

Techniques: Western Blot, Knockdown, Flow Cytometry

Journal: International Journal of Oral Science

Article Title: Porphyromonas gingivalis potentiates stem-like properties of oral squamous cell carcinoma by modulating SCD1-dependent lipid synthesis via NOD1/KLF5 axis

doi: 10.1038/s41368-024-00342-8

Figure Lengend Snippet: SCD1 upregulation in OSCC cells was transcriptionally activated by KLF5. a Venn diagram showing the candidate transcription factor screening process. b , c qRT-PCR showed P. gingivalis specifically promoted the expression of KLF5. d ChIP-qPCR showed the binding of KLF5 to the SCD1 promoter region in HSC-4 cells. e Dual-luciferase reporter assay showed the binding of KLF5 to the SCD1 promoter region in 293 T cells. f Western blot showed knockdown of KLF5 suppressed the protein level of SCD1. β-actin was used as a housekeeping gene. n = 3. Data are presented as the mean ± SD. ns: P > 0.05, * P < 0.05, ** P < 0.01

Article Snippet: The OSCC cell lines HSC-4 and SCC-9 were originally derived from a patient with tongue cancer and respectively obtained from the Japanese Collection of Research Bioresources Cell Bank (JCRB; Shinjuku, Japan) and American Type Culture Collection (ATCC, Manassas, USA).

Techniques: Quantitative RT-PCR, Expressing, ChIP-qPCR, Binding Assay, Luciferase, Reporter Assay, Western Blot, Knockdown

Journal: International Journal of Oral Science

Article Title: Porphyromonas gingivalis potentiates stem-like properties of oral squamous cell carcinoma by modulating SCD1-dependent lipid synthesis via NOD1/KLF5 axis

doi: 10.1038/s41368-024-00342-8

Figure Lengend Snippet: P. gingivalis stabilized KLF5 expression via the activation of NOD1. a Top twenty most enriched KEGG pathways among significantly upregulated DEGs in HSC-4 cells co-cultured with P. gingivalis . b qRT-PCR showed P. gingivalis promoted the NOD1 gene expression of OSCC cells. c – f Western blot and quantification showed silencing of NOD1 decreased the expression of KLF5 in OSCC cells. β-actin was used as a housekeeping gene. n = 3. Data are presented as the mean ± SD. ns: P > 0.05, * P < 0.05, ** P < 0.01

Article Snippet: The OSCC cell lines HSC-4 and SCC-9 were originally derived from a patient with tongue cancer and respectively obtained from the Japanese Collection of Research Bioresources Cell Bank (JCRB; Shinjuku, Japan) and American Type Culture Collection (ATCC, Manassas, USA).

Techniques: Expressing, Activation Assay, Cell Culture, Quantitative RT-PCR, Gene Expression, Western Blot

Journal: International Journal of Oral Science

Article Title: Porphyromonas gingivalis potentiates stem-like properties of oral squamous cell carcinoma by modulating SCD1-dependent lipid synthesis via NOD1/KLF5 axis

doi: 10.1038/s41368-024-00342-8

Figure Lengend Snippet: Schematic drawing of the role of P. gingivalis in regulating OSCC stemness. The specific molecular mechanism is that P. gingivalis promotes OSCC stemness by modulating SCD1-dependent lipid synthesis via NOD1/KLF5 axis. The figure was drawn using Figdraw

Article Snippet: The OSCC cell lines HSC-4 and SCC-9 were originally derived from a patient with tongue cancer and respectively obtained from the Japanese Collection of Research Bioresources Cell Bank (JCRB; Shinjuku, Japan) and American Type Culture Collection (ATCC, Manassas, USA).

Techniques: