Journal: Cell reports

Article Title: A Renewable Source of Human Beige Adipocytes for Development of Therapies to Treat Metabolic Syndrome

doi: 10.1016/j.celrep.2018.11.037

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Human: HprAD (subcutaneous) , Lonza , Cat#PT-5020.

Techniques: Staining, Recombinant, Saline, Passaging, Inhibition, Modification, Blocking Assay, Western Blot, XF Assay, SYBR Green Assay, Bicinchoninic Acid Protein Assay, Software, Cell Culture, Membrane

Journal: Cell reports

Article Title: A Renewable Source of Human Beige Adipocytes for Development of Therapies to Treat Metabolic Syndrome

doi: 10.1016/j.celrep.2018.11.037

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Human: D-HprAD (subcutaneous) , Lonza , Cat#PT-5022.

Techniques: Staining, Recombinant, Saline, Passaging, Inhibition, Modification, Blocking Assay, Western Blot, XF Assay, SYBR Green Assay, Bicinchoninic Acid Protein Assay, Software, Cell Culture, Membrane

Journal: International Journal of Molecular Medicine

Article Title: Metformin-suppressed differentiation of human visceral preadipocytes: Involvement of microRNAs

doi: 10.3892/ijmm.2016.2729

Figure Lengend Snippet: Metformin suppresses the maturation of human visceral preadipocytes with no suppression of cell growth. (A) Metformin suppresses lipid droplet accumulation in human visceral preadipocytes. Oil Red O staining of HPrAD-vis cells was performed after 2 weeks of culture with or without metformin. The stained area was reduced in metformin-treated cells compared to non-treated cells, ×200. (B) The relative stained area of metformin-treated cells and non-treated cells. Cells were cultured for 1 or 2 weeks with or without metformin. The stained areas per ×200 field were measured using Image J. (C) Adiponectin secretion from HPrAD-vis cells is decreased following treatment with metformin. The cells were cultured for 1 or 2 weeks with or without metformin (n=3). The adiponectin concentration in the culture media was determined by ELISA with the specific antibody Acrp30. (D) Metformin did not suppress the growth of fatty cells. Cells were incubated with or without metformin for 1 week. Cell proliferation was measured using a WST-8 assay (n=6). (E) Genes involved in the differentiation and maturation of preadipocytes are downregulated by metformin. Cells were incubated for 1 week with or without metformin. The relative expression of PPARγ and C/EBPα in metformin-treated cells compared to non-treated cells was determined using RT-qPCR with relative quantification (n=3). β-actin was used as an internal control gene. (F) Effect of metformin on gene expression was confirmed by western blot analysis. The cells were incubated for 1 week with or without metformin. The protein expression level of C/EBPα decreased after treatment. HPrAD-vis, human visceral preadipocytes; ELISA, enzyme-linked immunosorbent assay; PPARγ, peroxisome proliferator-activated receptor γ; C/EBPα, CCAAT-enhancer-binding protein α.

Article Snippet: PoieticsTM human visceral preadipocytes (HPrAD-vis) were purchased from Lonza (Walkersville, MD, USA).

Techniques: Staining, Cell Culture, Concentration Assay, Enzyme-linked Immunosorbent Assay, Incubation, Expressing, Quantitative RT-PCR, Quantitative Proteomics, Control, Gene Expression, Western Blot, Binding Assay

Journal: International Journal of Molecular Medicine

Article Title: Metformin-suppressed differentiation of human visceral preadipocytes: Involvement of microRNAs

doi: 10.3892/ijmm.2016.2729

Figure Lengend Snippet: Hierarchical clustering of miRNAs from HPrAD-vis cells. Hierarchical clustering was performed for miRNA expression profiles of control HPrAD-vis cells and cells cultured with 5 mM metformin for 1 (left-side panel) or 2 weeks (left-side panel). The samples are arranged in columns and miRNAs in rows. The miRNA clustering tree is shown on the left, and the sample clustering tree is shown at the top of each heat map. The heat maps show the relative expression intensity for each miRNA in which the base-2 logarithm of the intensity is median-centered for each row. The color-coding is indicated as a horizontal bar at the bottom left. The six miRNAs with an asterisk are common between data from 1 week incubation with metformin (P<0.05) and 2 weeks incubation with the reagent (P<0.005) regardless of fold-change (n=5). miRNAs, microRNAs; HPrAD-vis, human visceral preadipocytes.

Article Snippet: PoieticsTM human visceral preadipocytes (HPrAD-vis) were purchased from Lonza (Walkersville, MD, USA).

Techniques: Expressing, Control, Cell Culture, Incubation