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Pg-hWE alleviates PCOS symptoms in a mouse model based on H&E staining. (A) PCOS mouse model construction. The mice were subcutaneously administered with 30 mg/kg of DHEA for six weeks. One week prior to start of SC administration, each group was administered the appropriate drug (tap water, dextrin, and Pg-hWE) orally. (B) Body weight was measured once a week. (C) Results of the AMH ELISA with mouse serum. (D) Mouse ovary histology by H&E staining. (E) Results of ovarian follicles by type. # p < 0.05 ## p < 0.01 compared with the Normal group, * p < 0.05 and ⁎⁎ p < 0.01 compared with the PCOS group.

Journal: Integrative Medicine Research

Article Title: Therapeutic effects of pomegranate hot-water extract via inhibition of apoptosis and oxidative stress in a DHEA-induced mouse model of PCOS

doi: 10.1016/j.imr.2025.101264

Figure Lengend Snippet: Pg-hWE alleviates PCOS symptoms in a mouse model based on H&E staining. (A) PCOS mouse model construction. The mice were subcutaneously administered with 30 mg/kg of DHEA for six weeks. One week prior to start of SC administration, each group was administered the appropriate drug (tap water, dextrin, and Pg-hWE) orally. (B) Body weight was measured once a week. (C) Results of the AMH ELISA with mouse serum. (D) Mouse ovary histology by H&E staining. (E) Results of ovarian follicles by type. # p < 0.05 ## p < 0.01 compared with the Normal group, * p < 0.05 and ⁎⁎ p < 0.01 compared with the PCOS group.

Article Snippet: ELISA of mouse serum was performed using the CUSABIO's anti-Müllerian hormone ELISA kit (CUSABIO, Houston, TX, USA) as reported previously, and all preparation and experimental procedures were performed according to the protocol provided by CUSABIO.

Techniques: Staining, Enzyme-linked Immunosorbent Assay