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high-density peptide array  (PEPperPRINT gmbh)
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PEPperPRINT gmbh high-density peptide array
High Density Peptide Array, supplied by PEPperPRINT gmbh, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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high-density peptide array - by Bioz Stars, 2026-06
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high-density peptide array pepperchip microarray  (PEPperPRINT gmbh)
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PEPperPRINT gmbh high-density peptide array pepperchip microarray
High-density peptide arrays (HDPA) provide high-resolution antibody epitope maps across the SARS-CoV-2 proteome (A) Overview of analytical pipeline. The proteome of SARS-CoV-2 was translated into 15-mer overlapping peptides with a peptide-to-peptide overlap of 13 amino acids. The resulting individual peptides were printed in duplicates on the <t>microarray.</t> Sera from confirmed SARS-CoV-2-positive and -negative individuals were incubated on <t>PEPperCHIP</t> the HDPA. Serum antibody binding was visualized using respective fluorescently labeled secondary antibodies (anti-human IgG and anti-human IgA). Image acquisition and data quantification resulted in epitope-specific antibody profiles for SARS-CoV-2. (B) Average relative fluorescent units (RFU) profiles and peptide coverages are plotted across the SARS-CoV-2 proteome (ORF1A, ORF1B, Spike (S) protein, Envelope (E) protein, Membrane (M) glycoprotein, Nucleocapsid (N) phosphoprotein). Antibody responses to each linear 15-mer peptide were mapped across the SARS-CoV-2 proteome and average RFU calculated for each amino acid residue. The normalized positional ‘epitope coverage’ at each protein residue location is defined as the ratio of total peptides mapped to each position by the total expected peptides (see section). ‘Hotspots’ can be seen as spiked in the RFU or coverage distributions. (C) Comparison of mean RFU (log-scale) between SARS-CoV-2-positive and -negative sample groups for each viral protein. (unpaired t-test, ns: p > 0.05; ∗: p ≤ 0.05; ∗∗: p ≤ 0.01; ∗∗∗: p ≤ 0.001; ∗∗∗∗: p ≤ 0.0001).
High Density Peptide Array Pepperchip Microarray, supplied by PEPperPRINT gmbh, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/high-density peptide array pepperchip microarray/product/PEPperPRINT gmbh
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high-density peptide array pepperchip microarray - by Bioz Stars, 2026-06
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high-density peptide arrays  (PEPperPRINT gmbh)
90
PEPperPRINT gmbh high-density peptide arrays
High-density peptide arrays (HDPA) provide high-resolution antibody epitope maps across the SARS-CoV-2 proteome (A) Overview of analytical pipeline. The proteome of SARS-CoV-2 was translated into 15-mer overlapping peptides with a peptide-to-peptide overlap of 13 amino acids. The resulting individual peptides were printed in duplicates on the <t>microarray.</t> Sera from confirmed SARS-CoV-2-positive and -negative individuals were incubated on <t>PEPperCHIP</t> the HDPA. Serum antibody binding was visualized using respective fluorescently labeled secondary antibodies (anti-human IgG and anti-human IgA). Image acquisition and data quantification resulted in epitope-specific antibody profiles for SARS-CoV-2. (B) Average relative fluorescent units (RFU) profiles and peptide coverages are plotted across the SARS-CoV-2 proteome (ORF1A, ORF1B, Spike (S) protein, Envelope (E) protein, Membrane (M) glycoprotein, Nucleocapsid (N) phosphoprotein). Antibody responses to each linear 15-mer peptide were mapped across the SARS-CoV-2 proteome and average RFU calculated for each amino acid residue. The normalized positional ‘epitope coverage’ at each protein residue location is defined as the ratio of total peptides mapped to each position by the total expected peptides (see section). ‘Hotspots’ can be seen as spiked in the RFU or coverage distributions. (C) Comparison of mean RFU (log-scale) between SARS-CoV-2-positive and -negative sample groups for each viral protein. (unpaired t-test, ns: p > 0.05; ∗: p ≤ 0.05; ∗∗: p ≤ 0.01; ∗∗∗: p ≤ 0.001; ∗∗∗∗: p ≤ 0.0001).
High Density Peptide Arrays, supplied by PEPperPRINT gmbh, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/high-density peptide arrays/product/PEPperPRINT gmbh
Average 90 stars, based on 1 article reviews
high-density peptide arrays - by Bioz Stars, 2026-06
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high-density peptide array analysis  (PEPperPRINT gmbh)
90
PEPperPRINT gmbh high-density peptide array analysis
High-density peptide arrays (HDPA) provide high-resolution antibody epitope maps across the SARS-CoV-2 proteome (A) Overview of analytical pipeline. The proteome of SARS-CoV-2 was translated into 15-mer overlapping peptides with a peptide-to-peptide overlap of 13 amino acids. The resulting individual peptides were printed in duplicates on the <t>microarray.</t> Sera from confirmed SARS-CoV-2-positive and -negative individuals were incubated on <t>PEPperCHIP</t> the HDPA. Serum antibody binding was visualized using respective fluorescently labeled secondary antibodies (anti-human IgG and anti-human IgA). Image acquisition and data quantification resulted in epitope-specific antibody profiles for SARS-CoV-2. (B) Average relative fluorescent units (RFU) profiles and peptide coverages are plotted across the SARS-CoV-2 proteome (ORF1A, ORF1B, Spike (S) protein, Envelope (E) protein, Membrane (M) glycoprotein, Nucleocapsid (N) phosphoprotein). Antibody responses to each linear 15-mer peptide were mapped across the SARS-CoV-2 proteome and average RFU calculated for each amino acid residue. The normalized positional ‘epitope coverage’ at each protein residue location is defined as the ratio of total peptides mapped to each position by the total expected peptides (see section). ‘Hotspots’ can be seen as spiked in the RFU or coverage distributions. (C) Comparison of mean RFU (log-scale) between SARS-CoV-2-positive and -negative sample groups for each viral protein. (unpaired t-test, ns: p > 0.05; ∗: p ≤ 0.05; ∗∗: p ≤ 0.01; ∗∗∗: p ≤ 0.001; ∗∗∗∗: p ≤ 0.0001).
High Density Peptide Array Analysis, supplied by PEPperPRINT gmbh, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/high-density peptide array analysis/product/PEPperPRINT gmbh
Average 90 stars, based on 1 article reviews
high-density peptide array analysis - by Bioz Stars, 2026-06
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fluorescence polarization high density peptide arrays  (INTAVIS Inc)
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INTAVIS Inc fluorescence polarization high density peptide arrays
High-density peptide arrays (HDPA) provide high-resolution antibody epitope maps across the SARS-CoV-2 proteome (A) Overview of analytical pipeline. The proteome of SARS-CoV-2 was translated into 15-mer overlapping peptides with a peptide-to-peptide overlap of 13 amino acids. The resulting individual peptides were printed in duplicates on the <t>microarray.</t> Sera from confirmed SARS-CoV-2-positive and -negative individuals were incubated on <t>PEPperCHIP</t> the HDPA. Serum antibody binding was visualized using respective fluorescently labeled secondary antibodies (anti-human IgG and anti-human IgA). Image acquisition and data quantification resulted in epitope-specific antibody profiles for SARS-CoV-2. (B) Average relative fluorescent units (RFU) profiles and peptide coverages are plotted across the SARS-CoV-2 proteome (ORF1A, ORF1B, Spike (S) protein, Envelope (E) protein, Membrane (M) glycoprotein, Nucleocapsid (N) phosphoprotein). Antibody responses to each linear 15-mer peptide were mapped across the SARS-CoV-2 proteome and average RFU calculated for each amino acid residue. The normalized positional ‘epitope coverage’ at each protein residue location is defined as the ratio of total peptides mapped to each position by the total expected peptides (see section). ‘Hotspots’ can be seen as spiked in the RFU or coverage distributions. (C) Comparison of mean RFU (log-scale) between SARS-CoV-2-positive and -negative sample groups for each viral protein. (unpaired t-test, ns: p > 0.05; ∗: p ≤ 0.05; ∗∗: p ≤ 0.01; ∗∗∗: p ≤ 0.001; ∗∗∗∗: p ≤ 0.0001).
Fluorescence Polarization High Density Peptide Arrays, supplied by INTAVIS Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fluorescence polarization high density peptide arrays/product/INTAVIS Inc
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fluorescence polarization high density peptide arrays - by Bioz Stars, 2026-06
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High-density peptide arrays (HDPA) provide high-resolution antibody epitope maps across the SARS-CoV-2 proteome (A) Overview of analytical pipeline. The proteome of SARS-CoV-2 was translated into 15-mer overlapping peptides with a peptide-to-peptide overlap of 13 amino acids. The resulting individual peptides were printed in duplicates on the microarray. Sera from confirmed SARS-CoV-2-positive and -negative individuals were incubated on PEPperCHIP the HDPA. Serum antibody binding was visualized using respective fluorescently labeled secondary antibodies (anti-human IgG and anti-human IgA). Image acquisition and data quantification resulted in epitope-specific antibody profiles for SARS-CoV-2. (B) Average relative fluorescent units (RFU) profiles and peptide coverages are plotted across the SARS-CoV-2 proteome (ORF1A, ORF1B, Spike (S) protein, Envelope (E) protein, Membrane (M) glycoprotein, Nucleocapsid (N) phosphoprotein). Antibody responses to each linear 15-mer peptide were mapped across the SARS-CoV-2 proteome and average RFU calculated for each amino acid residue. The normalized positional ‘epitope coverage’ at each protein residue location is defined as the ratio of total peptides mapped to each position by the total expected peptides (see section). ‘Hotspots’ can be seen as spiked in the RFU or coverage distributions. (C) Comparison of mean RFU (log-scale) between SARS-CoV-2-positive and -negative sample groups for each viral protein. (unpaired t-test, ns: p > 0.05; ∗: p ≤ 0.05; ∗∗: p ≤ 0.01; ∗∗∗: p ≤ 0.001; ∗∗∗∗: p ≤ 0.0001).

Journal: iScience

Article Title: Selection for immune evasion in SARS-CoV-2 revealed by high-resolution epitope mapping and sequence analysis

doi: 10.1016/j.isci.2023.107394

Figure Lengend Snippet: High-density peptide arrays (HDPA) provide high-resolution antibody epitope maps across the SARS-CoV-2 proteome (A) Overview of analytical pipeline. The proteome of SARS-CoV-2 was translated into 15-mer overlapping peptides with a peptide-to-peptide overlap of 13 amino acids. The resulting individual peptides were printed in duplicates on the microarray. Sera from confirmed SARS-CoV-2-positive and -negative individuals were incubated on PEPperCHIP the HDPA. Serum antibody binding was visualized using respective fluorescently labeled secondary antibodies (anti-human IgG and anti-human IgA). Image acquisition and data quantification resulted in epitope-specific antibody profiles for SARS-CoV-2. (B) Average relative fluorescent units (RFU) profiles and peptide coverages are plotted across the SARS-CoV-2 proteome (ORF1A, ORF1B, Spike (S) protein, Envelope (E) protein, Membrane (M) glycoprotein, Nucleocapsid (N) phosphoprotein). Antibody responses to each linear 15-mer peptide were mapped across the SARS-CoV-2 proteome and average RFU calculated for each amino acid residue. The normalized positional ‘epitope coverage’ at each protein residue location is defined as the ratio of total peptides mapped to each position by the total expected peptides (see section). ‘Hotspots’ can be seen as spiked in the RFU or coverage distributions. (C) Comparison of mean RFU (log-scale) between SARS-CoV-2-positive and -negative sample groups for each viral protein. (unpaired t-test, ns: p > 0.05; ∗: p ≤ 0.05; ∗∗: p ≤ 0.01; ∗∗∗: p ≤ 0.001; ∗∗∗∗: p ≤ 0.0001).

Article Snippet: To analyze the antibody responses to SARS-CoV-2 at the epitope level we used a recently developed high-density peptide array (HDPA), the PEPperCHIP Microarray (PEPperPRINT GmbH, Germany), covering the proteome of the SARS-CoV-2 isolate Wuhan-Hu-1 as well as the four seasonal hCoVs OC43, HKU1, NL63 and 229E (see for accession numbers used).

Techniques: Microarray, Incubation, Binding Assay, Labeling, Membrane, Residue, Comparison