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Journal: Plant Direct

Article Title: CUT&Tag for High‐Resolution Epigenomic Profiling From a Low Amount of Arabidopsis Tissue

doi: 10.1002/pld3.70161

Figure Lengend Snippet: PCR cycle program for CUT&Tag.

Article Snippet: 35 In a PCR tube (Thermo Fisher Scientific #AB0451), mix 21 μL tagmented DNA, 2 μL i5 primer (10 μM) and 2 μL i7 primer (10 μM), and 25 μL NEBNext Hi‐Fi 2× PCR mix (NEB #M0541) to make a total final volume of 50 μL.

Techniques: