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PRP-exos treatment promotes proliferation and migration of <t>HFSCs.</t> (A) The morphology of PRP-exos was observed by TEM. (B) The exosomal markers were identified by Western blot. (C) Immunofluorescence was used to identify the <t>marker</t> <t>CK19</t> of rat HFSCs. (D and E) Calcein/PI staining and CCK-8 were used to detect proliferation of HFSCs (n = 3). (F) Wound healing assays were used to measure the migration capacity of HFSCs (n = 3). ∗p < 0.05 compared with Control; ∗∗p < 0.01 compared with Control. Data are expressed as Mean ± SD.
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PRP-exos treatment promotes proliferation and migration of <t>HFSCs.</t> (A) The morphology of PRP-exos was observed by TEM. (B) The exosomal markers were identified by Western blot. (C) Immunofluorescence was used to identify the <t>marker</t> <t>CK19</t> of rat HFSCs. (D and E) Calcein/PI staining and CCK-8 were used to detect proliferation of HFSCs (n = 3). (F) Wound healing assays were used to measure the migration capacity of HFSCs (n = 3). ∗p < 0.05 compared with Control; ∗∗p < 0.01 compared with Control. Data are expressed as Mean ± SD.
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PRP-exos treatment promotes proliferation and migration of <t>HFSCs.</t> (A) The morphology of PRP-exos was observed by TEM. (B) The exosomal markers were identified by Western blot. (C) Immunofluorescence was used to identify the <t>marker</t> <t>CK19</t> of rat HFSCs. (D and E) Calcein/PI staining and CCK-8 were used to detect proliferation of HFSCs (n = 3). (F) Wound healing assays were used to measure the migration capacity of HFSCs (n = 3). ∗p < 0.05 compared with Control; ∗∗p < 0.01 compared with Control. Data are expressed as Mean ± SD.
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PRP-exos treatment promotes proliferation and migration of <t>HFSCs.</t> (A) The morphology of PRP-exos was observed by TEM. (B) The exosomal markers were identified by Western blot. (C) Immunofluorescence was used to identify the <t>marker</t> <t>CK19</t> of rat HFSCs. (D and E) Calcein/PI staining and CCK-8 were used to detect proliferation of HFSCs (n = 3). (F) Wound healing assays were used to measure the migration capacity of HFSCs (n = 3). ∗p < 0.05 compared with Control; ∗∗p < 0.01 compared with Control. Data are expressed as Mean ± SD.
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PRP-exos treatment promotes proliferation and migration of <t>HFSCs.</t> (A) The morphology of PRP-exos was observed by TEM. (B) The exosomal markers were identified by Western blot. (C) Immunofluorescence was used to identify the <t>marker</t> <t>CK19</t> of rat HFSCs. (D and E) Calcein/PI staining and CCK-8 were used to detect proliferation of HFSCs (n = 3). (F) Wound healing assays were used to measure the migration capacity of HFSCs (n = 3). ∗p < 0.05 compared with Control; ∗∗p < 0.01 compared with Control. Data are expressed as Mean ± SD.
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PRP-exos treatment promotes proliferation and migration of <t>HFSCs.</t> (A) The morphology of PRP-exos was observed by TEM. (B) The exosomal markers were identified by Western blot. (C) Immunofluorescence was used to identify the <t>marker</t> <t>CK19</t> of rat HFSCs. (D and E) Calcein/PI staining and CCK-8 were used to detect proliferation of HFSCs (n = 3). (F) Wound healing assays were used to measure the migration capacity of HFSCs (n = 3). ∗p < 0.05 compared with Control; ∗∗p < 0.01 compared with Control. Data are expressed as Mean ± SD.
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PRP-exos treatment promotes proliferation and migration of <t>HFSCs.</t> (A) The morphology of PRP-exos was observed by TEM. (B) The exosomal markers were identified by Western blot. (C) Immunofluorescence was used to identify the <t>marker</t> <t>CK19</t> of rat HFSCs. (D and E) Calcein/PI staining and CCK-8 were used to detect proliferation of HFSCs (n = 3). (F) Wound healing assays were used to measure the migration capacity of HFSCs (n = 3). ∗p < 0.05 compared with Control; ∗∗p < 0.01 compared with Control. Data are expressed as Mean ± SD.
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PRP-exos treatment promotes proliferation and migration of <t>HFSCs.</t> (A) The morphology of PRP-exos was observed by TEM. (B) The exosomal markers were identified by Western blot. (C) Immunofluorescence was used to identify the <t>marker</t> <t>CK19</t> of rat HFSCs. (D and E) Calcein/PI staining and CCK-8 were used to detect proliferation of HFSCs (n = 3). (F) Wound healing assays were used to measure the migration capacity of HFSCs (n = 3). ∗p < 0.05 compared with Control; ∗∗p < 0.01 compared with Control. Data are expressed as Mean ± SD.
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PRP-exos treatment promotes proliferation and migration of HFSCs. (A) The morphology of PRP-exos was observed by TEM. (B) The exosomal markers were identified by Western blot. (C) Immunofluorescence was used to identify the marker CK19 of rat HFSCs. (D and E) Calcein/PI staining and CCK-8 were used to detect proliferation of HFSCs (n = 3). (F) Wound healing assays were used to measure the migration capacity of HFSCs (n = 3). ∗p < 0.05 compared with Control; ∗∗p < 0.01 compared with Control. Data are expressed as Mean ± SD.

Journal: Regenerative Therapy

Article Title: Exosomes derived from platelet-rich plasma promote hair regeneration by regulating the SIRT1/FoxO3a pathway to alleviate oxidative stress

doi: 10.1016/j.reth.2025.08.005

Figure Lengend Snippet: PRP-exos treatment promotes proliferation and migration of HFSCs. (A) The morphology of PRP-exos was observed by TEM. (B) The exosomal markers were identified by Western blot. (C) Immunofluorescence was used to identify the marker CK19 of rat HFSCs. (D and E) Calcein/PI staining and CCK-8 were used to detect proliferation of HFSCs (n = 3). (F) Wound healing assays were used to measure the migration capacity of HFSCs (n = 3). ∗p < 0.05 compared with Control; ∗∗p < 0.01 compared with Control. Data are expressed as Mean ± SD.

Article Snippet: The rat HFSCs were purchased from Procell (China), and their marker CK19 was identified by immunofluorescence.

Techniques: Migration, Western Blot, Immunofluorescence, Marker, Staining, CCK-8 Assay, Control