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hfpr3  (R&D Systems)
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Hfpr3, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hfpr3  (Biotechnology Information)
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Biotechnology Information hfpr3
Western blotting detection of cell-free produced GPCRs. After the reactions, the samples were centrifuged. The supernatant containing the solubilized protein was removed, and the pellet was resuspended in an equivalent volume of buffer. The solubilized protein and resuspended pellets were detected by Western blotting. As controls, reactions with no peptide or the nonsurfactant peptide (IT)5 were performed. (A) Western blot of solubilized <t>hFPR3</t> in the presence of different peptides. (B) Western blot of solubilized hOR17-210 produced in the presence of different peptides. Similar results were obtained with hTAAR5 and mOR103-15.
Hfpr3, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Western blotting detection of cell-free produced GPCRs. After the reactions, the samples were centrifuged. The supernatant containing the solubilized protein was removed, and the pellet was resuspended in an equivalent volume of buffer. The solubilized protein and resuspended pellets were detected by Western blotting. As controls, reactions with no peptide or the nonsurfactant peptide (IT)5 were performed. (A) Western blot of solubilized hFPR3 in the presence of different peptides. (B) Western blot of solubilized hOR17-210 produced in the presence of different peptides. Similar results were obtained with hTAAR5 and mOR103-15.

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Peptide surfactants for cell-free production of functional G protein-coupled receptors

doi: 10.1073/pnas.1018185108

Figure Lengend Snippet: Western blotting detection of cell-free produced GPCRs. After the reactions, the samples were centrifuged. The supernatant containing the solubilized protein was removed, and the pellet was resuspended in an equivalent volume of buffer. The solubilized protein and resuspended pellets were detected by Western blotting. As controls, reactions with no peptide or the nonsurfactant peptide (IT)5 were performed. (A) Western blot of solubilized hFPR3 in the presence of different peptides. (B) Western blot of solubilized hOR17-210 produced in the presence of different peptides. Similar results were obtained with hTAAR5 and mOR103-15.

Article Snippet: Protein sequences of the following four GPCRs were obtained from the National Center for Biotechnology Information online database: hOR17-210 (UniProt release no. {"type":"entrez-protein","attrs":{"text":"Q8WZA6","term_id":"166215840","term_text":"Q8WZA6"}} Q8WZA6 ), mOR103-15 (NP release no. 035113.1), hTAAR5 (NP release no. 003958.2), and hFPR3 (NP release no. 002021.3).

Techniques: Western Blot, Produced

CD spectra of four GPCRs produced in the presence of peptide surfactants using a commercial E. coli cell-free system. (A) hFPR3 produced in Ac-VVVK-CONH2. (B) hTAAR5 produced in Ac-IIIK-CONH2. (C) mOR103-15 produced in Ac-VVVD-OH. (D) hOR17-210 produced in Ac-AAAAAAD-OH. These receptors all have characteristic α-helical spectra with valleys at 208 and 222 nm. Because GPCRs have 7-transmembrane α-helical domains, the CD spectra indicate that the receptors are properly folded.

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Peptide surfactants for cell-free production of functional G protein-coupled receptors

doi: 10.1073/pnas.1018185108

Figure Lengend Snippet: CD spectra of four GPCRs produced in the presence of peptide surfactants using a commercial E. coli cell-free system. (A) hFPR3 produced in Ac-VVVK-CONH2. (B) hTAAR5 produced in Ac-IIIK-CONH2. (C) mOR103-15 produced in Ac-VVVD-OH. (D) hOR17-210 produced in Ac-AAAAAAD-OH. These receptors all have characteristic α-helical spectra with valleys at 208 and 222 nm. Because GPCRs have 7-transmembrane α-helical domains, the CD spectra indicate that the receptors are properly folded.

Article Snippet: Protein sequences of the following four GPCRs were obtained from the National Center for Biotechnology Information online database: hOR17-210 (UniProt release no. {"type":"entrez-protein","attrs":{"text":"Q8WZA6","term_id":"166215840","term_text":"Q8WZA6"}} Q8WZA6 ), mOR103-15 (NP release no. 035113.1), hTAAR5 (NP release no. 003958.2), and hFPR3 (NP release no. 002021.3).

Techniques: Circular Dichroism, Produced