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hfl 1 cells  (ATCC)
96
ATCC hfl 1 cells
Proof-of-concept activity of FAP-CAR-NK cells in a fibrotic human lung organoid co-culture model. ( A ) Bright-field images showing morphological alterations in lung organoid co-cultures under non-fibrotic control conditions (NC), profibrotic stimulation (PC), pirfenidone treatment (PFD), untransduced NK-cell treatment (NK), and FAP-CAR-NK-cell treatment (CAR-NK). Treatment-associated fragmented, debris-like material is particularly evident in the NK and CAR-NK groups. Images were acquired at 4× and 10× magnification. ( B ) Multichannel fluorescence imaging of the three-dimensional Matrigel microdroplet co-culture system showing lung organoids labeled with CellTracker Blue CMAC <t>(blue),</t> <t>HFL-1</t> fibroblasts labeled with GFP (green), and effector cells labeled with CellTracker Deep Red (red); lower panels show magnified views of representative droplets (24 h). ( C ) RT-qPCR analysis of COL1A1, FAP, ACTA2, and SP-C expression in the indicated groups. qPCR primers are shown in . ( D ) Quantification of IFN-γ and TNF-α in culture supernatants after co-culture. Data are presented as mean ± SEM from three independently established organoid co-culture experiments ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns, not significant.
Hfl 1 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hfl 1 cells/product/ATCC
Average 96 stars, based on 1 article reviews
hfl 1 cells - by Bioz Stars, 2026-05
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hfl 1  (ATCC)
96
ATCC hfl 1
Proof-of-concept activity of FAP-CAR-NK cells in a fibrotic human lung organoid co-culture model. ( A ) Bright-field images showing morphological alterations in lung organoid co-cultures under non-fibrotic control conditions (NC), profibrotic stimulation (PC), pirfenidone treatment (PFD), untransduced NK-cell treatment (NK), and FAP-CAR-NK-cell treatment (CAR-NK). Treatment-associated fragmented, debris-like material is particularly evident in the NK and CAR-NK groups. Images were acquired at 4× and 10× magnification. ( B ) Multichannel fluorescence imaging of the three-dimensional Matrigel microdroplet co-culture system showing lung organoids labeled with CellTracker Blue CMAC <t>(blue),</t> <t>HFL-1</t> fibroblasts labeled with GFP (green), and effector cells labeled with CellTracker Deep Red (red); lower panels show magnified views of representative droplets (24 h). ( C ) RT-qPCR analysis of COL1A1, FAP, ACTA2, and SP-C expression in the indicated groups. qPCR primers are shown in . ( D ) Quantification of IFN-γ and TNF-α in culture supernatants after co-culture. Data are presented as mean ± SEM from three independently established organoid co-culture experiments ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns, not significant.
Hfl 1, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hfl 1/product/ATCC
Average 96 stars, based on 1 article reviews
hfl 1 - by Bioz Stars, 2026-05
96/100 stars
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human embryonic lung fibroblasts hfl 1  (Procell Inc)
86
Procell Inc human embryonic lung fibroblasts hfl 1
Proof-of-concept activity of FAP-CAR-NK cells in a fibrotic human lung organoid co-culture model. ( A ) Bright-field images showing morphological alterations in lung organoid co-cultures under non-fibrotic control conditions (NC), profibrotic stimulation (PC), pirfenidone treatment (PFD), untransduced NK-cell treatment (NK), and FAP-CAR-NK-cell treatment (CAR-NK). Treatment-associated fragmented, debris-like material is particularly evident in the NK and CAR-NK groups. Images were acquired at 4× and 10× magnification. ( B ) Multichannel fluorescence imaging of the three-dimensional Matrigel microdroplet co-culture system showing lung organoids labeled with CellTracker Blue CMAC <t>(blue),</t> <t>HFL-1</t> fibroblasts labeled with GFP (green), and effector cells labeled with CellTracker Deep Red (red); lower panels show magnified views of representative droplets (24 h). ( C ) RT-qPCR analysis of COL1A1, FAP, ACTA2, and SP-C expression in the indicated groups. qPCR primers are shown in . ( D ) Quantification of IFN-γ and TNF-α in culture supernatants after co-culture. Data are presented as mean ± SEM from three independently established organoid co-culture experiments ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns, not significant.
Human Embryonic Lung Fibroblasts Hfl 1, supplied by Procell Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human embryonic lung fibroblasts hfl 1/product/Procell Inc
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human embryonic lung fibroblasts hfl 1 - by Bioz Stars, 2026-05
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hfl 1  (Procell Inc)
86
Procell Inc hfl 1
Proof-of-concept activity of FAP-CAR-NK cells in a fibrotic human lung organoid co-culture model. ( A ) Bright-field images showing morphological alterations in lung organoid co-cultures under non-fibrotic control conditions (NC), profibrotic stimulation (PC), pirfenidone treatment (PFD), untransduced NK-cell treatment (NK), and FAP-CAR-NK-cell treatment (CAR-NK). Treatment-associated fragmented, debris-like material is particularly evident in the NK and CAR-NK groups. Images were acquired at 4× and 10× magnification. ( B ) Multichannel fluorescence imaging of the three-dimensional Matrigel microdroplet co-culture system showing lung organoids labeled with CellTracker Blue CMAC <t>(blue),</t> <t>HFL-1</t> fibroblasts labeled with GFP (green), and effector cells labeled with CellTracker Deep Red (red); lower panels show magnified views of representative droplets (24 h). ( C ) RT-qPCR analysis of COL1A1, FAP, ACTA2, and SP-C expression in the indicated groups. qPCR primers are shown in . ( D ) Quantification of IFN-γ and TNF-α in culture supernatants after co-culture. Data are presented as mean ± SEM from three independently established organoid co-culture experiments ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns, not significant.
Hfl 1, supplied by Procell Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hfl 1/product/Procell Inc
Average 86 stars, based on 1 article reviews
hfl 1 - by Bioz Stars, 2026-05
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human riken hibepic human riken hfl  (ATCC)
96
ATCC human riken hibepic human riken hfl
Proof-of-concept activity of FAP-CAR-NK cells in a fibrotic human lung organoid co-culture model. ( A ) Bright-field images showing morphological alterations in lung organoid co-cultures under non-fibrotic control conditions (NC), profibrotic stimulation (PC), pirfenidone treatment (PFD), untransduced NK-cell treatment (NK), and FAP-CAR-NK-cell treatment (CAR-NK). Treatment-associated fragmented, debris-like material is particularly evident in the NK and CAR-NK groups. Images were acquired at 4× and 10× magnification. ( B ) Multichannel fluorescence imaging of the three-dimensional Matrigel microdroplet co-culture system showing lung organoids labeled with CellTracker Blue CMAC <t>(blue),</t> <t>HFL-1</t> fibroblasts labeled with GFP (green), and effector cells labeled with CellTracker Deep Red (red); lower panels show magnified views of representative droplets (24 h). ( C ) RT-qPCR analysis of COL1A1, FAP, ACTA2, and SP-C expression in the indicated groups. qPCR primers are shown in . ( D ) Quantification of IFN-γ and TNF-α in culture supernatants after co-culture. Data are presented as mean ± SEM from three independently established organoid co-culture experiments ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns, not significant.
Human Riken Hibepic Human Riken Hfl, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human riken hibepic human riken hfl/product/ATCC
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human riken hibepic human riken hfl - by Bioz Stars, 2026-05
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human fetal lung fibroblasts hfl 1  (ATCC)
96
ATCC human fetal lung fibroblasts hfl 1
Proof-of-concept activity of FAP-CAR-NK cells in a fibrotic human lung organoid co-culture model. ( A ) Bright-field images showing morphological alterations in lung organoid co-cultures under non-fibrotic control conditions (NC), profibrotic stimulation (PC), pirfenidone treatment (PFD), untransduced NK-cell treatment (NK), and FAP-CAR-NK-cell treatment (CAR-NK). Treatment-associated fragmented, debris-like material is particularly evident in the NK and CAR-NK groups. Images were acquired at 4× and 10× magnification. ( B ) Multichannel fluorescence imaging of the three-dimensional Matrigel microdroplet co-culture system showing lung organoids labeled with CellTracker Blue CMAC <t>(blue),</t> <t>HFL-1</t> fibroblasts labeled with GFP (green), and effector cells labeled with CellTracker Deep Red (red); lower panels show magnified views of representative droplets (24 h). ( C ) RT-qPCR analysis of COL1A1, FAP, ACTA2, and SP-C expression in the indicated groups. qPCR primers are shown in . ( D ) Quantification of IFN-γ and TNF-α in culture supernatants after co-culture. Data are presented as mean ± SEM from three independently established organoid co-culture experiments ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns, not significant.
Human Fetal Lung Fibroblasts Hfl 1, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human fetal lung fibroblasts hfl 1/product/ATCC
Average 96 stars, based on 1 article reviews
human fetal lung fibroblasts hfl 1 - by Bioz Stars, 2026-05
96/100 stars
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Image Search Results


Proof-of-concept activity of FAP-CAR-NK cells in a fibrotic human lung organoid co-culture model. ( A ) Bright-field images showing morphological alterations in lung organoid co-cultures under non-fibrotic control conditions (NC), profibrotic stimulation (PC), pirfenidone treatment (PFD), untransduced NK-cell treatment (NK), and FAP-CAR-NK-cell treatment (CAR-NK). Treatment-associated fragmented, debris-like material is particularly evident in the NK and CAR-NK groups. Images were acquired at 4× and 10× magnification. ( B ) Multichannel fluorescence imaging of the three-dimensional Matrigel microdroplet co-culture system showing lung organoids labeled with CellTracker Blue CMAC (blue), HFL-1 fibroblasts labeled with GFP (green), and effector cells labeled with CellTracker Deep Red (red); lower panels show magnified views of representative droplets (24 h). ( C ) RT-qPCR analysis of COL1A1, FAP, ACTA2, and SP-C expression in the indicated groups. qPCR primers are shown in . ( D ) Quantification of IFN-γ and TNF-α in culture supernatants after co-culture. Data are presented as mean ± SEM from three independently established organoid co-culture experiments ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns, not significant.

Journal: International Journal of Molecular Sciences

Article Title: Proof-of-Concept Evaluation of Primary Human FAP-CAR-NK Cells Targeting Activated Fibroblasts in Pulmonary Fibrosis

doi: 10.3390/ijms27094128

Figure Lengend Snippet: Proof-of-concept activity of FAP-CAR-NK cells in a fibrotic human lung organoid co-culture model. ( A ) Bright-field images showing morphological alterations in lung organoid co-cultures under non-fibrotic control conditions (NC), profibrotic stimulation (PC), pirfenidone treatment (PFD), untransduced NK-cell treatment (NK), and FAP-CAR-NK-cell treatment (CAR-NK). Treatment-associated fragmented, debris-like material is particularly evident in the NK and CAR-NK groups. Images were acquired at 4× and 10× magnification. ( B ) Multichannel fluorescence imaging of the three-dimensional Matrigel microdroplet co-culture system showing lung organoids labeled with CellTracker Blue CMAC (blue), HFL-1 fibroblasts labeled with GFP (green), and effector cells labeled with CellTracker Deep Red (red); lower panels show magnified views of representative droplets (24 h). ( C ) RT-qPCR analysis of COL1A1, FAP, ACTA2, and SP-C expression in the indicated groups. qPCR primers are shown in . ( D ) Quantification of IFN-γ and TNF-α in culture supernatants after co-culture. Data are presented as mean ± SEM from three independently established organoid co-culture experiments ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns, not significant.

Article Snippet: Phoenix-AMPHO and HFL-1 cells were obtained from the American Type Culture Collection (ATCC).

Techniques: Activity Assay, Co-Culture Assay, Control, Fluorescence, Imaging, Labeling, Quantitative RT-PCR, Expressing