Journal: Scientific Reports

Article Title: Primary cilia suppress the fibrotic activity of atrial fibroblasts from patients with atrial fibrillation in vitro

doi: 10.1038/s41598-024-60298-x

Figure Lengend Snippet: PHA promotes the cilia formation and increase the length of primary cilia in NHCF-A cells. ( a ) The proportion of NHCF-A cells with primary cilia cultured in the growth medium supplemented with 10%FBS, hFGF-B and insulin following a PHA treatment at different doses (0, 0.5, 1.0 or 2.0 μM) for 48 h. N = 2/treatment. Error bars, mean (SD). ( b – d ) The proportion of ciliated NHCF-A cells ( b ), the representative morphology ( c ) and the length of primary cilia ( d ) when NHCF-A cells were cultured in 0.5% FBS medium with vehicle or 2 ng/mL TGF-β1 in the absence (DMSO) or presence of 2 μg/mL PHA. Arrow heads indicate primary cilia. Red: ac α-tub (primary cilia), blue: DAPI (nuclei). Scale bar, 20 μm. In total, > 100 primary cilia/treatment from three technical replicates were measured. Error bars, mean (SD). ** p < 0.01 in ( b ) (Student’s unpaired t -test). *** p < 0.001 in ( c ) (Mann–Whitney U test).

Article Snippet: Normal Heart Cardiac Fibroblasts from Atria (hereafter NHCF-A cells) were purchased from LONZA (Cat#. CC-2903) and cultured in the growth medium; FGM™-3 added with hFGF-B and insulin (Cat#. CC-4526).

Techniques: Cell Culture, MANN-WHITNEY