Journal: Molecular Metabolism

Article Title: Nutrient-driven histone acetylation underlies energy storage and mobilization

doi: 10.1016/j.molmet.2026.102344

Figure Lengend Snippet: BHB-mediated HDAC inhibition shifts hepatic anabolism toward lipid oxidation. (A) Experimental design. (B) Serum gross appearance and the FFA levels ( n = 5). (C) Body weight loss of mice with the indicated treatment ( n = 5). (D and E) ChIP-seq data displaying H3K27ac (D) or BRD4 (E) occupancy at two subsets of genes: H3K27ac and BRD4 enriched at the genomic loci of fasting-induced transcripts (energy mobilization associated genes, such as Cyp4a14), but dislodged from those loci of fasting-suppressed transcripts (energy storage-associated genes, such as Pcsk9). (ref. to Hsieh et al., 2022, Mol Cell for detail method of analysis). (F and G) GO enrichment analysis of the fasting-induced H3K27ac (F) and BRD4 (G) peak-associated genes. The reads number of the ChIP-seq data for H3K27ac (log2FC > 1 and P < 0.01) or BRD4 (log2FC > 1.2 and P < 0.01) were included as the fasting-induced peaks. (H and I) Gene tracks display that fasting induced the occupancy of H3K27ac and BRD4 at the genomic loci of energy mobilization-associated genes (Cyp4a14, Pck1) (H), but reduced at those loci of energy storage-associated genes (Pcsk9, Srebf1) (I). (J) The ratio of the liver to body weight (upper panel) and the liver triglycerides levels (lower panel) of mice with the indicated treatment ( n = 5). (K-O) Body weight loss data (K), serum gross appearance and FFA levels (L), the blood glucose and BHB levels (M), the ratio of liver to body weight (N) and Liver triglycerides (O)of Brd 4 -flox or Brd4 hKO mice subjected to 24 h of fasting ( n = 5). Data are shown as the mean ± SEM. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001 by unpaired two-tailed Student’s t-test (K-O) or one-way ANOVA followed with Bonferroni’s multiple comparisons test (B, C, and J).

Article Snippet: Biochemical parameters, including serum and hepatic triglycerides (AKFA003M, BOXBIO), FFA (E-BC-K792-M, Elabscience), and ALT (E-BC-K235-M96T, Elabscience) were measured using commercial kits according to the manufacturer’s instructions.

Techniques: Inhibition, ChIP-sequencing, Two Tailed Test

Journal: Poultry Science

Article Title: Research note: Effects of dietary bile acids supplementation on hepatic lipid accumulation and fatty acid profile of hens at late laying cycle

doi: 10.1016/j.psj.2025.106026

Figure Lengend Snippet: Effects of bile acids supplementation on liver histopathology and mRNA expression of lipid metabolism-related genes in the hens at late laying cycle. (A) Hematoxylin and eosin photomicrographs obtained at 40 × (Ⅰ-Ⅳ) and 400 × (Ⅴ-Ⅷ) magnification; (B) HSL , hormone-sensitive triglyceride lipase; (C) SCD , stearoyl-CoA desaturase-1; (D) ADD1 , adipocyte determination and differentiation factor-1; (E) LPL , lipoprteinlipase; (F) FAS , fatty acid synthetase; (G) FABP4 , fatty acid-binding protein 4. Control, BA200, BA300, and BA500 represent the basal diet added with 0, 200, 300, and 500 mg/kg bile acids, respectively. a,b Means with different lowercase letters differ significantly among the four treatments ( P < 0.05). Lin and Quad showed linear and quadratic effects of different dietary BAs levels. n = 6.

Article Snippet: Hepatic triglyceride ( TC ; CAS#A110-1-1), total cholesterol ( TG ; CAS#A111-1-1), and MDA (CAS#A003-1-2) levels were measured using the commercial assay kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) according to the manufacturer's protocols.

Techniques: Histopathology, Expressing, Binding Assay, Control