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Example of target illuminating the eye of a Dutch-belted rabbit with a <t>green</t> <t>HeNe</t> laser centered in the crosshairs and collinear with the exposing laser. The second green spot located above the center is a reflection. A suture can be seen in the top-right. The target diameter was ∼ 5 mm . Keeping the target image in focus meant the cornea was at the beam waist [exposure plane] of the laser. A live video of this view was captured and displayed for staff to help determine a good exposure site devoid of imperfections such as dryness or previous exposures as the animal was maneuvered on the five-axis stage.
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Example of target illuminating the eye of a Dutch-belted rabbit with a <t>green</t> <t>HeNe</t> laser centered in the crosshairs and collinear with the exposing laser. The second green spot located above the center is a reflection. A suture can be seen in the top-right. The target diameter was ∼ 5 mm . Keeping the target image in focus meant the cornea was at the beam waist [exposure plane] of the laser. A live video of this view was captured and displayed for staff to help determine a good exposure site devoid of imperfections such as dryness or previous exposures as the animal was maneuvered on the five-axis stage.
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Example of target illuminating the eye of a Dutch-belted rabbit with a green HeNe laser centered in the crosshairs and collinear with the exposing laser. The second green spot located above the center is a reflection. A suture can be seen in the top-right. The target diameter was ∼ 5 mm . Keeping the target image in focus meant the cornea was at the beam waist [exposure plane] of the laser. A live video of this view was captured and displayed for staff to help determine a good exposure site devoid of imperfections such as dryness or previous exposures as the animal was maneuvered on the five-axis stage.

Journal: Journal of Biomedical Optics

Article Title: Corneal dose response from exposure to a Q -switched laser at a central wavelength of 1645 nm using a rabbit model

doi: 10.1117/1.JBO.31.5.054703

Figure Lengend Snippet: Example of target illuminating the eye of a Dutch-belted rabbit with a green HeNe laser centered in the crosshairs and collinear with the exposing laser. The second green spot located above the center is a reflection. A suture can be seen in the top-right. The target diameter was ∼ 5 mm . Keeping the target image in focus meant the cornea was at the beam waist [exposure plane] of the laser. A live video of this view was captured and displayed for staff to help determine a good exposure site devoid of imperfections such as dryness or previous exposures as the animal was maneuvered on the five-axis stage.

Article Snippet: A green HeNe laser (LDS5, Thorlabs) was also set collinear with the exposure laser that was center to the target crosshairs as further aiming assistance.

Techniques: