Journal: Redox Biology

Article Title: Oxidative stress-driven m 5 C methylation by NSUN5 confers cisplatin resistance in lung adenocarcinoma through promoting glycolysis

doi: 10.1016/j.redox.2026.104193

Figure Lengend Snippet: NSUN5 promotes glycolysis and HR through GLUT1. (A) The glucose uptake was measured in NSUN5-overexpressing A549 cells with shNC or shGLUT1 transfection by fluorescently labeled glucose analogue 2-NBDG. The nucleus (blue) was stained with Hoechst. Scale bars, 100 μm. (B) Glycolytic flux analysis by extracellular acidification rate (ECAR). Real-time ECAR tracing in A549 cells sequentially treated with glucose, oligomycin (oligo), and 2-DG across experimental groups (left panel). Quantification of glycolytic parameters, including the basal glycolytic rate, maximal glycolytic capacity, and spare glycolytic capacity (right panel). (C) Mitochondrial respiration analysis by oxygen consumption rate (OCR). Real-time OCR tracing in A549 cells sequentially treated with oligomycin, FCCP, and rotenone/antimycin A across groups (left panel). Quantification of mitochondrial parameters, including basal respiration, ATP production, maximal respiration, and spare respiratory capacity (right panel). (D) Relative lactate production in designated A549 cell groups. (E) Schematic representation of the HR reporter. (F) The HR levels of the indicated HEK293T cells were detected by flow cytometry. (G-J) Representative immunofluorescence images of MRE11 (G), p -RPA2 (H), BrdU (I), and RAD51 (J) foci in A549 cells under indicated treatments. Scale bars, 10 μm. Data were representative of at least three independent experiments and presented as mean (SD). Statistical significance was determined using Student's t-test (B-D, F-J), ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, n.s, not significant.

Article Snippet: HEK293T (RRID: CVCL_0063) cells were purchased from Procell (Wuhan, China).

Techniques: Transfection, Labeling, Staining, Flow Cytometry, Immunofluorescence

Journal: iScience

Article Title: Single-granule profiling reveals that RNP granule pH marks cellular translation

doi: 10.1016/j.isci.2026.116203

Figure Lengend Snippet: Variation in pH of RNP granules and translation activity of cells under stresses (A) Schematic of stress treatment and indicator detection. (B) pH values of P-bodies and SGs under different stress conditions. Data are represented as mean ± SD ( n = 5 cells with at least 12 granules). (C) Schematic of changes in translation activity in cells exposed to stresses. (D) Cell viability of HEK293T cells facing different stresses. Data are represented as mean ± SD ( n = 3). (E) Principle of nascent protein synthesis detection. (F and G) Levels of protein synthesis in HEK293T cells under different stresses. Data in (F) are represented as mean ± SD ( n = 3). Images were representative examples from three independent experiments. Scale bars in (G), 40 μm. Statistical significance was assessed using a non-paired two-tailed t test in (B), (D), and (F) (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001).

Article Snippet: Human: HEK293T , Procell , RRID: CVCL_0063.

Techniques: Activity Assay, Two Tailed Test