Review





Similar Products

hek293 gp cells  (ATCC)
99
ATCC hek293 gp cells
Hek293 Gp Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hek293+gp/293/pmc10169838__41467_2023_37863_MOESM2_ESM-28-0-19
Average 99 stars, based on 1 article reviews
hek293 gp cells - by Bioz Stars, 2026-09
99/100 stars
  Buy from Supplier
hek293 gp  (TaKaRa)
94
TaKaRa hek293 gp
( A ) HeLa CDC14B-YFP and HeLa YFP -inducible cell lines were grown in heavy and light medium, respectively, to compare the phospho-proteome by mass spectrometry (MS). m / z , mass/charge ratio; IMAC, immobilized metal affinity chromatography. ( B ) Molecular network of the hypophosphorylated proteins (hits) from (A). The network was constructed in Cytoscape to extract functional information and shows hits (bold font) and their most related genes (regular font) identified using a guilt-by-association approach. Coexpression and shared protein domains are denoted by purple and yellow lines, respectively. The identified phosphorylation sites are summarized in table S1. ( C ) Gene Ontology (GO) analysis of hypophosphorylated proteins from (B). FDR, false discovery rate. ( D ) Sequence logos of hypophosphorylated p-sites from (A). ( E ) RPE1 WT and RPE1 CDC14B KO cells were treated with 3.3 μM NOC for 24 hours to accumulate prometaphase cells with high MeCP2 pS92 signal. The MeCP2 pS92 /MeCP2 ratio was densitometrically measured and is depicted in the graph on the right-hand side of the immunoblot. The asterisk indicates nonspecific protein bands that are detected by the anti-MeCP2 pS92 antibody. Green lines depict position of specific bands. The quantification was performed on three independent experiments. ( F ) RPE1 Tet ON cells expressing CDC14B-mSc or the catalytic inactive CDC14B C314S -mSc were treated and analyzed as described in (E). Asterisk and lines as in (E). The quantification was performed on three independent experiments. a.u., arbitrary units. ( G ) In vitro dephosphorylation of MeCP2 pS92 by purified CDC14B. <t>HEK293T</t> cells were transfected with MeCP2-FLAG . MeCP2-FLAG protein was immunoprecipitated using FLAG beads and incubated with recombinant CDC14B-GFP or catalytic inactive CDC14B C314S -GFP in vitro. The MeCP2 pS92 /MeCP2 ratio was densitometrically measured and is depicted in the graph next to the immunoblot. The quantification was performed on three independent experiments. IP, immunoprecipitation. (E to G) Results are means ± SD. Two-tailed unpaired t test. n.s. (not significant) P > 0.05, ** P < 0.01, and **** P < 0.0001.
Hek293 Gp, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hek293+gp/HEK+293+Tet-Off+Advanced+Cell+Line/pmc09858502-132-7-9
Average 94 stars, based on 1 article reviews
hek293 gp - by Bioz Stars, 2026-09
94/100 stars
  Buy from Supplier
hek293 gp cells  (TaKaRa)
86
TaKaRa hek293 gp cells
( A ) HeLa CDC14B-YFP and HeLa YFP -inducible cell lines were grown in heavy and light medium, respectively, to compare the phospho-proteome by mass spectrometry (MS). m / z , mass/charge ratio; IMAC, immobilized metal affinity chromatography. ( B ) Molecular network of the hypophosphorylated proteins (hits) from (A). The network was constructed in Cytoscape to extract functional information and shows hits (bold font) and their most related genes (regular font) identified using a guilt-by-association approach. Coexpression and shared protein domains are denoted by purple and yellow lines, respectively. The identified phosphorylation sites are summarized in table S1. ( C ) Gene Ontology (GO) analysis of hypophosphorylated proteins from (B). FDR, false discovery rate. ( D ) Sequence logos of hypophosphorylated p-sites from (A). ( E ) RPE1 WT and RPE1 CDC14B KO cells were treated with 3.3 μM NOC for 24 hours to accumulate prometaphase cells with high MeCP2 pS92 signal. The MeCP2 pS92 /MeCP2 ratio was densitometrically measured and is depicted in the graph on the right-hand side of the immunoblot. The asterisk indicates nonspecific protein bands that are detected by the anti-MeCP2 pS92 antibody. Green lines depict position of specific bands. The quantification was performed on three independent experiments. ( F ) RPE1 Tet ON cells expressing CDC14B-mSc or the catalytic inactive CDC14B C314S -mSc were treated and analyzed as described in (E). Asterisk and lines as in (E). The quantification was performed on three independent experiments. a.u., arbitrary units. ( G ) In vitro dephosphorylation of MeCP2 pS92 by purified CDC14B. <t>HEK293T</t> cells were transfected with MeCP2-FLAG . MeCP2-FLAG protein was immunoprecipitated using FLAG beads and incubated with recombinant CDC14B-GFP or catalytic inactive CDC14B C314S -GFP in vitro. The MeCP2 pS92 /MeCP2 ratio was densitometrically measured and is depicted in the graph next to the immunoblot. The quantification was performed on three independent experiments. IP, immunoprecipitation. (E to G) Results are means ± SD. Two-tailed unpaired t test. n.s. (not significant) P > 0.05, ** P < 0.01, and **** P < 0.0001.
Hek293 Gp Cells, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hek293+gp/pmc08193631__ja0c13235_si_001-77-30-32
Average 86 stars, based on 1 article reviews
hek293 gp cells - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier
hek293 atcc cat  (ATCC)
94
ATCC hek293 atcc cat
( A ) HeLa CDC14B-YFP and HeLa YFP -inducible cell lines were grown in heavy and light medium, respectively, to compare the phospho-proteome by mass spectrometry (MS). m / z , mass/charge ratio; IMAC, immobilized metal affinity chromatography. ( B ) Molecular network of the hypophosphorylated proteins (hits) from (A). The network was constructed in Cytoscape to extract functional information and shows hits (bold font) and their most related genes (regular font) identified using a guilt-by-association approach. Coexpression and shared protein domains are denoted by purple and yellow lines, respectively. The identified phosphorylation sites are summarized in table S1. ( C ) Gene Ontology (GO) analysis of hypophosphorylated proteins from (B). FDR, false discovery rate. ( D ) Sequence logos of hypophosphorylated p-sites from (A). ( E ) RPE1 WT and RPE1 CDC14B KO cells were treated with 3.3 μM NOC for 24 hours to accumulate prometaphase cells with high MeCP2 pS92 signal. The MeCP2 pS92 /MeCP2 ratio was densitometrically measured and is depicted in the graph on the right-hand side of the immunoblot. The asterisk indicates nonspecific protein bands that are detected by the anti-MeCP2 pS92 antibody. Green lines depict position of specific bands. The quantification was performed on three independent experiments. ( F ) RPE1 Tet ON cells expressing CDC14B-mSc or the catalytic inactive CDC14B C314S -mSc were treated and analyzed as described in (E). Asterisk and lines as in (E). The quantification was performed on three independent experiments. a.u., arbitrary units. ( G ) In vitro dephosphorylation of MeCP2 pS92 by purified CDC14B. <t>HEK293T</t> cells were transfected with MeCP2-FLAG . MeCP2-FLAG protein was immunoprecipitated using FLAG beads and incubated with recombinant CDC14B-GFP or catalytic inactive CDC14B C314S -GFP in vitro. The MeCP2 pS92 /MeCP2 ratio was densitometrically measured and is depicted in the graph next to the immunoblot. The quantification was performed on three independent experiments. IP, immunoprecipitation. (E to G) Results are means ± SD. Two-tailed unpaired t test. n.s. (not significant) P > 0.05, ** P < 0.01, and **** P < 0.0001.
Hek293 Atcc Cat, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hek293+gp/GP%2BE-86/10__2139_slash_ssrn__3915012-668-73-74
Average 94 stars, based on 1 article reviews
hek293 atcc cat - by Bioz Stars, 2026-09
94/100 stars
  Buy from Supplier
commercial hek293-derived ev samples hbmhek293-gp  (HansaBioMed ltd)
90
HansaBioMed ltd commercial hek293-derived ev samples hbmhek293-gp
( A ) HeLa CDC14B-YFP and HeLa YFP -inducible cell lines were grown in heavy and light medium, respectively, to compare the phospho-proteome by mass spectrometry (MS). m / z , mass/charge ratio; IMAC, immobilized metal affinity chromatography. ( B ) Molecular network of the hypophosphorylated proteins (hits) from (A). The network was constructed in Cytoscape to extract functional information and shows hits (bold font) and their most related genes (regular font) identified using a guilt-by-association approach. Coexpression and shared protein domains are denoted by purple and yellow lines, respectively. The identified phosphorylation sites are summarized in table S1. ( C ) Gene Ontology (GO) analysis of hypophosphorylated proteins from (B). FDR, false discovery rate. ( D ) Sequence logos of hypophosphorylated p-sites from (A). ( E ) RPE1 WT and RPE1 CDC14B KO cells were treated with 3.3 μM NOC for 24 hours to accumulate prometaphase cells with high MeCP2 pS92 signal. The MeCP2 pS92 /MeCP2 ratio was densitometrically measured and is depicted in the graph on the right-hand side of the immunoblot. The asterisk indicates nonspecific protein bands that are detected by the anti-MeCP2 pS92 antibody. Green lines depict position of specific bands. The quantification was performed on three independent experiments. ( F ) RPE1 Tet ON cells expressing CDC14B-mSc or the catalytic inactive CDC14B C314S -mSc were treated and analyzed as described in (E). Asterisk and lines as in (E). The quantification was performed on three independent experiments. a.u., arbitrary units. ( G ) In vitro dephosphorylation of MeCP2 pS92 by purified CDC14B. <t>HEK293T</t> cells were transfected with MeCP2-FLAG . MeCP2-FLAG protein was immunoprecipitated using FLAG beads and incubated with recombinant CDC14B-GFP or catalytic inactive CDC14B C314S -GFP in vitro. The MeCP2 pS92 /MeCP2 ratio was densitometrically measured and is depicted in the graph next to the immunoblot. The quantification was performed on three independent experiments. IP, immunoprecipitation. (E to G) Results are means ± SD. Two-tailed unpaired t test. n.s. (not significant) P > 0.05, ** P < 0.01, and **** P < 0.0001.
Commercial Hek293 Derived Ev Samples Hbmhek293 Gp, supplied by HansaBioMed ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hek293+gp/hek+293+large+ev/pmc07452272__mmc1-136-15-18
Average 90 stars, based on 1 article reviews
commercial hek293-derived ev samples hbmhek293-gp - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
hek293-gp-tgr5 overexpressing cells  (Cellgro)
90
Cellgro hek293-gp-tgr5 overexpressing cells
( A ) HeLa CDC14B-YFP and HeLa YFP -inducible cell lines were grown in heavy and light medium, respectively, to compare the phospho-proteome by mass spectrometry (MS). m / z , mass/charge ratio; IMAC, immobilized metal affinity chromatography. ( B ) Molecular network of the hypophosphorylated proteins (hits) from (A). The network was constructed in Cytoscape to extract functional information and shows hits (bold font) and their most related genes (regular font) identified using a guilt-by-association approach. Coexpression and shared protein domains are denoted by purple and yellow lines, respectively. The identified phosphorylation sites are summarized in table S1. ( C ) Gene Ontology (GO) analysis of hypophosphorylated proteins from (B). FDR, false discovery rate. ( D ) Sequence logos of hypophosphorylated p-sites from (A). ( E ) RPE1 WT and RPE1 CDC14B KO cells were treated with 3.3 μM NOC for 24 hours to accumulate prometaphase cells with high MeCP2 pS92 signal. The MeCP2 pS92 /MeCP2 ratio was densitometrically measured and is depicted in the graph on the right-hand side of the immunoblot. The asterisk indicates nonspecific protein bands that are detected by the anti-MeCP2 pS92 antibody. Green lines depict position of specific bands. The quantification was performed on three independent experiments. ( F ) RPE1 Tet ON cells expressing CDC14B-mSc or the catalytic inactive CDC14B C314S -mSc were treated and analyzed as described in (E). Asterisk and lines as in (E). The quantification was performed on three independent experiments. a.u., arbitrary units. ( G ) In vitro dephosphorylation of MeCP2 pS92 by purified CDC14B. <t>HEK293T</t> cells were transfected with MeCP2-FLAG . MeCP2-FLAG protein was immunoprecipitated using FLAG beads and incubated with recombinant CDC14B-GFP or catalytic inactive CDC14B C314S -GFP in vitro. The MeCP2 pS92 /MeCP2 ratio was densitometrically measured and is depicted in the graph next to the immunoblot. The quantification was performed on three independent experiments. IP, immunoprecipitation. (E to G) Results are means ± SD. Two-tailed unpaired t test. n.s. (not significant) P > 0.05, ** P < 0.01, and **** P < 0.0001.
Hek293 Gp Tgr5 Overexpressing Cells, supplied by Cellgro, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hek293+gp/hek293t+cells/us10703761-2124-5-18
Average 90 stars, based on 1 article reviews
hek293-gp-tgr5 overexpressing cells - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
recombinant human bile salt efflux pump (bsep, expressed in sf9 or hek293 cells), p-gp, and bcrp  (Solvo Biotechnology)
90
Solvo Biotechnology recombinant human bile salt efflux pump (bsep, expressed in sf9 or hek293 cells), p-gp, and bcrp
( A ) HeLa CDC14B-YFP and HeLa YFP -inducible cell lines were grown in heavy and light medium, respectively, to compare the phospho-proteome by mass spectrometry (MS). m / z , mass/charge ratio; IMAC, immobilized metal affinity chromatography. ( B ) Molecular network of the hypophosphorylated proteins (hits) from (A). The network was constructed in Cytoscape to extract functional information and shows hits (bold font) and their most related genes (regular font) identified using a guilt-by-association approach. Coexpression and shared protein domains are denoted by purple and yellow lines, respectively. The identified phosphorylation sites are summarized in table S1. ( C ) Gene Ontology (GO) analysis of hypophosphorylated proteins from (B). FDR, false discovery rate. ( D ) Sequence logos of hypophosphorylated p-sites from (A). ( E ) RPE1 WT and RPE1 CDC14B KO cells were treated with 3.3 μM NOC for 24 hours to accumulate prometaphase cells with high MeCP2 pS92 signal. The MeCP2 pS92 /MeCP2 ratio was densitometrically measured and is depicted in the graph on the right-hand side of the immunoblot. The asterisk indicates nonspecific protein bands that are detected by the anti-MeCP2 pS92 antibody. Green lines depict position of specific bands. The quantification was performed on three independent experiments. ( F ) RPE1 Tet ON cells expressing CDC14B-mSc or the catalytic inactive CDC14B C314S -mSc were treated and analyzed as described in (E). Asterisk and lines as in (E). The quantification was performed on three independent experiments. a.u., arbitrary units. ( G ) In vitro dephosphorylation of MeCP2 pS92 by purified CDC14B. <t>HEK293T</t> cells were transfected with MeCP2-FLAG . MeCP2-FLAG protein was immunoprecipitated using FLAG beads and incubated with recombinant CDC14B-GFP or catalytic inactive CDC14B C314S -GFP in vitro. The MeCP2 pS92 /MeCP2 ratio was densitometrically measured and is depicted in the graph next to the immunoblot. The quantification was performed on three independent experiments. IP, immunoprecipitation. (E to G) Results are means ± SD. Two-tailed unpaired t test. n.s. (not significant) P > 0.05, ** P < 0.01, and **** P < 0.0001.
Recombinant Human Bile Salt Efflux Pump (Bsep, Expressed In Sf9 Or Hek293 Cells), P Gp, And Bcrp, supplied by Solvo Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hek293+gp/oatp2b1+expressing+mdckii/pm28281384-40-18-22
Average 90 stars, based on 1 article reviews
recombinant human bile salt efflux pump (bsep, expressed in sf9 or hek293 cells), p-gp, and bcrp - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
hek293 derived virus packaging cell line phoenix gp  (ATCC)
95
ATCC hek293 derived virus packaging cell line phoenix gp
( A ) HeLa CDC14B-YFP and HeLa YFP -inducible cell lines were grown in heavy and light medium, respectively, to compare the phospho-proteome by mass spectrometry (MS). m / z , mass/charge ratio; IMAC, immobilized metal affinity chromatography. ( B ) Molecular network of the hypophosphorylated proteins (hits) from (A). The network was constructed in Cytoscape to extract functional information and shows hits (bold font) and their most related genes (regular font) identified using a guilt-by-association approach. Coexpression and shared protein domains are denoted by purple and yellow lines, respectively. The identified phosphorylation sites are summarized in table S1. ( C ) Gene Ontology (GO) analysis of hypophosphorylated proteins from (B). FDR, false discovery rate. ( D ) Sequence logos of hypophosphorylated p-sites from (A). ( E ) RPE1 WT and RPE1 CDC14B KO cells were treated with 3.3 μM NOC for 24 hours to accumulate prometaphase cells with high MeCP2 pS92 signal. The MeCP2 pS92 /MeCP2 ratio was densitometrically measured and is depicted in the graph on the right-hand side of the immunoblot. The asterisk indicates nonspecific protein bands that are detected by the anti-MeCP2 pS92 antibody. Green lines depict position of specific bands. The quantification was performed on three independent experiments. ( F ) RPE1 Tet ON cells expressing CDC14B-mSc or the catalytic inactive CDC14B C314S -mSc were treated and analyzed as described in (E). Asterisk and lines as in (E). The quantification was performed on three independent experiments. a.u., arbitrary units. ( G ) In vitro dephosphorylation of MeCP2 pS92 by purified CDC14B. <t>HEK293T</t> cells were transfected with MeCP2-FLAG . MeCP2-FLAG protein was immunoprecipitated using FLAG beads and incubated with recombinant CDC14B-GFP or catalytic inactive CDC14B C314S -GFP in vitro. The MeCP2 pS92 /MeCP2 ratio was densitometrically measured and is depicted in the graph next to the immunoblot. The quantification was performed on three independent experiments. IP, immunoprecipitation. (E to G) Results are means ± SD. Two-tailed unpaired t test. n.s. (not significant) P > 0.05, ** P < 0.01, and **** P < 0.0001.
Hek293 Derived Virus Packaging Cell Line Phoenix Gp, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hek293+gp/Phoenix-GP/10__1128_slash_mcb__00620___10-45-1-18
Average 95 stars, based on 1 article reviews
hek293 derived virus packaging cell line phoenix gp - by Bioz Stars, 2026-09
95/100 stars
  Buy from Supplier

Image Search Results


( A ) HeLa CDC14B-YFP and HeLa YFP -inducible cell lines were grown in heavy and light medium, respectively, to compare the phospho-proteome by mass spectrometry (MS). m / z , mass/charge ratio; IMAC, immobilized metal affinity chromatography. ( B ) Molecular network of the hypophosphorylated proteins (hits) from (A). The network was constructed in Cytoscape to extract functional information and shows hits (bold font) and their most related genes (regular font) identified using a guilt-by-association approach. Coexpression and shared protein domains are denoted by purple and yellow lines, respectively. The identified phosphorylation sites are summarized in table S1. ( C ) Gene Ontology (GO) analysis of hypophosphorylated proteins from (B). FDR, false discovery rate. ( D ) Sequence logos of hypophosphorylated p-sites from (A). ( E ) RPE1 WT and RPE1 CDC14B KO cells were treated with 3.3 μM NOC for 24 hours to accumulate prometaphase cells with high MeCP2 pS92 signal. The MeCP2 pS92 /MeCP2 ratio was densitometrically measured and is depicted in the graph on the right-hand side of the immunoblot. The asterisk indicates nonspecific protein bands that are detected by the anti-MeCP2 pS92 antibody. Green lines depict position of specific bands. The quantification was performed on three independent experiments. ( F ) RPE1 Tet ON cells expressing CDC14B-mSc or the catalytic inactive CDC14B C314S -mSc were treated and analyzed as described in (E). Asterisk and lines as in (E). The quantification was performed on three independent experiments. a.u., arbitrary units. ( G ) In vitro dephosphorylation of MeCP2 pS92 by purified CDC14B. HEK293T cells were transfected with MeCP2-FLAG . MeCP2-FLAG protein was immunoprecipitated using FLAG beads and incubated with recombinant CDC14B-GFP or catalytic inactive CDC14B C314S -GFP in vitro. The MeCP2 pS92 /MeCP2 ratio was densitometrically measured and is depicted in the graph next to the immunoblot. The quantification was performed on three independent experiments. IP, immunoprecipitation. (E to G) Results are means ± SD. Two-tailed unpaired t test. n.s. (not significant) P > 0.05, ** P < 0.01, and **** P < 0.0001.

Journal: Science Advances

Article Title: The HIPK2/CDC14B-MeCP2 axis enhances the spindle assembly checkpoint block by promoting cyclin B translation

doi: 10.1126/sciadv.add6982

Figure Lengend Snippet: ( A ) HeLa CDC14B-YFP and HeLa YFP -inducible cell lines were grown in heavy and light medium, respectively, to compare the phospho-proteome by mass spectrometry (MS). m / z , mass/charge ratio; IMAC, immobilized metal affinity chromatography. ( B ) Molecular network of the hypophosphorylated proteins (hits) from (A). The network was constructed in Cytoscape to extract functional information and shows hits (bold font) and their most related genes (regular font) identified using a guilt-by-association approach. Coexpression and shared protein domains are denoted by purple and yellow lines, respectively. The identified phosphorylation sites are summarized in table S1. ( C ) Gene Ontology (GO) analysis of hypophosphorylated proteins from (B). FDR, false discovery rate. ( D ) Sequence logos of hypophosphorylated p-sites from (A). ( E ) RPE1 WT and RPE1 CDC14B KO cells were treated with 3.3 μM NOC for 24 hours to accumulate prometaphase cells with high MeCP2 pS92 signal. The MeCP2 pS92 /MeCP2 ratio was densitometrically measured and is depicted in the graph on the right-hand side of the immunoblot. The asterisk indicates nonspecific protein bands that are detected by the anti-MeCP2 pS92 antibody. Green lines depict position of specific bands. The quantification was performed on three independent experiments. ( F ) RPE1 Tet ON cells expressing CDC14B-mSc or the catalytic inactive CDC14B C314S -mSc were treated and analyzed as described in (E). Asterisk and lines as in (E). The quantification was performed on three independent experiments. a.u., arbitrary units. ( G ) In vitro dephosphorylation of MeCP2 pS92 by purified CDC14B. HEK293T cells were transfected with MeCP2-FLAG . MeCP2-FLAG protein was immunoprecipitated using FLAG beads and incubated with recombinant CDC14B-GFP or catalytic inactive CDC14B C314S -GFP in vitro. The MeCP2 pS92 /MeCP2 ratio was densitometrically measured and is depicted in the graph next to the immunoblot. The quantification was performed on three independent experiments. IP, immunoprecipitation. (E to G) Results are means ± SD. Two-tailed unpaired t test. n.s. (not significant) P > 0.05, ** P < 0.01, and **** P < 0.0001.

Article Snippet: RPE1, human embryonic kidney (HEK) 293T, and HEK293-GP (GP2-293, Clontech) cells were cultured in Gibco Dulbecco’s Modified Eagle’s medium (DMEM)/F-12 (Thermo Fisher Scientific) medium supplemented with 10% fetal bovine serum (FBS), 1% l -glutamine, and 1% penicillin/streptomycin.

Techniques: Mass Spectrometry, Affinity Chromatography, Construct, Functional Assay, Sequencing, Western Blot, Expressing, In Vitro, De-Phosphorylation Assay, Purification, Transfection, Immunoprecipitation, Incubation, Recombinant, Two Tailed Test