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(A) PDMS devices fabricated from soft lithography molds assembled onto MatTek glass-bottom culture dishes with integrated removable PDMS rods, and resultant lumen channels following rod removal after collagen polymerization. (B) Schematic showing the lumen seeding arrangement with one lumen seeded with human dermal lymphatic <t>endothelial</t> cells <t>(HdLECs,</t> yellow) to form a biomimetic lymphatic vessel and the adjacent lumen loaded with neutrophils (purple). pHrodo-conjugated bacterial bioparticles (green) are embedded within the surrounding collagen matrix. (C) Representative fluorescence image of a biomimetic lymphatic vessel formed within the collagen matrix.
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(A) PDMS devices fabricated from soft lithography molds assembled onto MatTek glass-bottom culture dishes with integrated removable PDMS rods, and resultant lumen channels following rod removal after collagen polymerization. (B) Schematic showing the lumen seeding arrangement with one lumen seeded with human dermal lymphatic <t>endothelial</t> cells <t>(HdLECs,</t> yellow) to form a biomimetic lymphatic vessel and the adjacent lumen loaded with neutrophils (purple). pHrodo-conjugated bacterial bioparticles (green) are embedded within the surrounding collagen matrix. (C) Representative fluorescence image of a biomimetic lymphatic vessel formed within the collagen matrix.
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(A) PDMS devices fabricated from soft lithography molds assembled onto MatTek glass-bottom culture dishes with integrated removable PDMS rods, and resultant lumen channels following rod removal after collagen polymerization. (B) Schematic showing the lumen seeding arrangement with one lumen seeded with human dermal lymphatic endothelial cells (HdLECs, yellow) to form a biomimetic lymphatic vessel and the adjacent lumen loaded with neutrophils (purple). pHrodo-conjugated bacterial bioparticles (green) are embedded within the surrounding collagen matrix. (C) Representative fluorescence image of a biomimetic lymphatic vessel formed within the collagen matrix.

Journal: bioRxiv

Article Title: Lymphatic Endothelial Cells Regulate Neutrophil Phenotypes and Function in a Microphysiological Model of Infection

doi: 10.64898/2026.03.24.714048

Figure Lengend Snippet: (A) PDMS devices fabricated from soft lithography molds assembled onto MatTek glass-bottom culture dishes with integrated removable PDMS rods, and resultant lumen channels following rod removal after collagen polymerization. (B) Schematic showing the lumen seeding arrangement with one lumen seeded with human dermal lymphatic endothelial cells (HdLECs, yellow) to form a biomimetic lymphatic vessel and the adjacent lumen loaded with neutrophils (purple). pHrodo-conjugated bacterial bioparticles (green) are embedded within the surrounding collagen matrix. (C) Representative fluorescence image of a biomimetic lymphatic vessel formed within the collagen matrix.

Article Snippet: Adult primary human dermal lymphatic endothelial cells (HdLECs) were purchased from PromoCell (C-12217).

Techniques: Fluorescence