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Trends in histone deacylase activity toward metabolic acylations. (A) Log 10 transformed V /[ E ] (min –1 ) of sirtuins 1, 2, and 6 and MiDAC toward 147 bp nucleosomes with one to eight carbon acylations of H3K9. (B) Log 10 transformed V /[ E ] (min –1 ) of class I and class III <t>HDACs</t> toward 147 bp nucleosomes with four carbon metabolism-linked acylations of H3K9. (C) Log 10 transformed V /[ E ] (min –1 ) of class I and class III HDACs toward 147 bp nucleosomes with H3K9 succinylation.
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hdacs mitosis-associated hdac complex (midac)  (MIDAC Corporation)
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( A ) Workflow for isolating tandem mass tagged histone H3 tails with cW11 sortase. ( B ) SDS-PAGE of 14-hour sortase reaction in a nuclear acid extract. ( C ) Tris-Tricine PAGE of histone H3(1-34) synthetic standards (1: 140 ng; 2: 420 ng) and TCA precipitated H3(1-32)-TMT from the acid extract reaction; bands between 25 and 37 kDa are histone H1. ( D ) Structure of the TMT-labelled oligoglycine peptide illustrating sortase reactive GGG, charge carrying K and carboxamide, and TMT labelled aminoalanine. ( E ) Unique proteoforms detected in each 6plex sample and shared between the two samples. ( F ) Quantifiable proteoforms detected in each 6plex sample and shared between the two samples. Volcano plot of significant (p<0.05) log2-fold change and overall abundance of H3 proteoforms in HEK293T cells following treatment with ( G <t>)</t> <t>LSD1/HDAC1/CoREST</t> complex-specific inhibitor Corin, or ( H ) pan class one <t>HDAC</t> inhibitor MS275.
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hdacs mitosis-associated hdac complex (midac) - by Bioz Stars, 2026-09
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hdac complexes midac  (MIDAC Corporation)
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( A ) Workflow for isolating tandem mass tagged histone H3 tails with cW11 sortase. ( B ) SDS-PAGE of 14-hour sortase reaction in a nuclear acid extract. ( C ) Tris-Tricine PAGE of histone H3(1-34) synthetic standards (1: 140 ng; 2: 420 ng) and TCA precipitated H3(1-32)-TMT from the acid extract reaction; bands between 25 and 37 kDa are histone H1. ( D ) Structure of the TMT-labelled oligoglycine peptide illustrating sortase reactive GGG, charge carrying K and carboxamide, and TMT labelled aminoalanine. ( E ) Unique proteoforms detected in each 6plex sample and shared between the two samples. ( F ) Quantifiable proteoforms detected in each 6plex sample and shared between the two samples. Volcano plot of significant (p<0.05) log2-fold change and overall abundance of H3 proteoforms in HEK293T cells following treatment with ( G <t>)</t> <t>LSD1/HDAC1/CoREST</t> complex-specific inhibitor Corin, or ( H ) pan class one <t>HDAC</t> inhibitor MS275.
Hdac Complexes Midac, supplied by MIDAC Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hdac+complexes+midac/hdac+complexes+midac/pm36584580-54-26-24
Average 90 stars, based on 1 article reviews
hdac complexes midac - by Bioz Stars, 2026-09
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Trends in histone deacylase activity toward metabolic acylations. (A) Log 10 transformed V /[ E ] (min –1 ) of sirtuins 1, 2, and 6 and MiDAC toward 147 bp nucleosomes with one to eight carbon acylations of H3K9. (B) Log 10 transformed V /[ E ] (min –1 ) of class I and class III HDACs toward 147 bp nucleosomes with four carbon metabolism-linked acylations of H3K9. (C) Log 10 transformed V /[ E ] (min –1 ) of class I and class III HDACs toward 147 bp nucleosomes with H3K9 succinylation.

Journal: Journal of the American Chemical Society

Article Title: Circular Engineered Sortase for Interrogating Histone H3 in Chromatin

doi: 10.1021/jacs.4c12585

Figure Lengend Snippet: Trends in histone deacylase activity toward metabolic acylations. (A) Log 10 transformed V /[ E ] (min –1 ) of sirtuins 1, 2, and 6 and MiDAC toward 147 bp nucleosomes with one to eight carbon acylations of H3K9. (B) Log 10 transformed V /[ E ] (min –1 ) of class I and class III HDACs toward 147 bp nucleosomes with four carbon metabolism-linked acylations of H3K9. (C) Log 10 transformed V /[ E ] (min –1 ) of class I and class III HDACs toward 147 bp nucleosomes with H3K9 succinylation.

Article Snippet: Drawing upon previously reported histone acylation selectivity and proposed modes of nucleosome recognition, we selected the nuclear or nucleocytoplasmic HDACs, mitosis-associated HDAC complex (MiDAC) and LSD1/HDAC/CoREST (LHC) complex, as representatives of class I HDAC complexes, and sirtuins 1, 2, and 6 as representatives of class III HDACs with which to explore the class- or complex-specific modes of deacylation., , , Across all sites on the H3 tail, K9 is generally the most rapidly deacetylated and was therefore selected to probe reactivity trends.

Techniques: Activity Assay, Transformation Assay

( A ) Workflow for isolating tandem mass tagged histone H3 tails with cW11 sortase. ( B ) SDS-PAGE of 14-hour sortase reaction in a nuclear acid extract. ( C ) Tris-Tricine PAGE of histone H3(1-34) synthetic standards (1: 140 ng; 2: 420 ng) and TCA precipitated H3(1-32)-TMT from the acid extract reaction; bands between 25 and 37 kDa are histone H1. ( D ) Structure of the TMT-labelled oligoglycine peptide illustrating sortase reactive GGG, charge carrying K and carboxamide, and TMT labelled aminoalanine. ( E ) Unique proteoforms detected in each 6plex sample and shared between the two samples. ( F ) Quantifiable proteoforms detected in each 6plex sample and shared between the two samples. Volcano plot of significant (p<0.05) log2-fold change and overall abundance of H3 proteoforms in HEK293T cells following treatment with ( G ) LSD1/HDAC1/CoREST complex-specific inhibitor Corin, or ( H ) pan class one HDAC inhibitor MS275.

Journal: bioRxiv

Article Title: A circular engineered sortase for interrogating histone H3 in chromatin

doi: 10.1101/2024.09.10.612318

Figure Lengend Snippet: ( A ) Workflow for isolating tandem mass tagged histone H3 tails with cW11 sortase. ( B ) SDS-PAGE of 14-hour sortase reaction in a nuclear acid extract. ( C ) Tris-Tricine PAGE of histone H3(1-34) synthetic standards (1: 140 ng; 2: 420 ng) and TCA precipitated H3(1-32)-TMT from the acid extract reaction; bands between 25 and 37 kDa are histone H1. ( D ) Structure of the TMT-labelled oligoglycine peptide illustrating sortase reactive GGG, charge carrying K and carboxamide, and TMT labelled aminoalanine. ( E ) Unique proteoforms detected in each 6plex sample and shared between the two samples. ( F ) Quantifiable proteoforms detected in each 6plex sample and shared between the two samples. Volcano plot of significant (p<0.05) log2-fold change and overall abundance of H3 proteoforms in HEK293T cells following treatment with ( G ) LSD1/HDAC1/CoREST complex-specific inhibitor Corin, or ( H ) pan class one HDAC inhibitor MS275.

Article Snippet: Drawing upon previously reported histone acylation selectivity and proposed modes of nucleosome-recognition, we selected the nuclear or nucleocytoplasmic HDACs mitosis-associated HDAC complex (MiDAC) and LSD1/HDAC/CoREST (LHC) complex as representatives of class I HDAC complexes, and Sirtuins 1, 2, and 6 as representatives of class III HDACs with which to explore the class- or complex-specific modes of deacylation., , , Across all sites on the H3 tail K9 is generally the most rapidly deacetylated, and was therefore selected to probe reactivity trends.

Techniques: SDS Page