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hct116 brca2 -/- cells  (Johns Hopkins HealthCare)
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Antitumoral efficacy of NDI-NIs in advanced 3D models. Advanced 3D spheroids were established from human colorectal cancer cells <t>(HCT116)</t> by growing the cells within drops of a methylcellulose scaffold matrix (day 0). After 2 days of culture (day 2), the spheres were treated with the indicated compound at final concentrations of 1, 2, and 5 μM. ( A ) Time course analysis of tumor spheroids growth, starting from 24 h after treatment (day 3), monitored by Incucyte ® S3 Live-Cell Analysis System (Essen BioScience, Ann Arbor, MI), 10× magnification. The results were expressed as the percentage of the spheroids area upon treatment relative to their own area before the compound administration. The histogram represents the mean values ± S.E.M. of at least four spheres. ( B ) Representative images of tumor spheroids growth described in panel (A). ( C ) Histological and immunohistochemical analysis of the spheroids generated from HCT116 cell lines and grown as in panel (A). Upper panels , representative images of immunostained sections. Lower panels, quantification of Ki-67, cleaved-caspase and γH2AX levels, expressed as percentage of positive cells. Thirty fields for condition were analyzed. The histograms represent the mean values ± S.D. of three independent experiments. * P < .05, ** P < .01, *** P < .001, and **** P < .0001.
Hct116 Brca2 / Cells, supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hct116 brca2−/− cell-derived xenografts  (Charles River Laboratories)
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Antitumoral efficacy of NDI-NIs in advanced 3D models. Advanced 3D spheroids were established from human colorectal cancer cells <t>(HCT116)</t> by growing the cells within drops of a methylcellulose scaffold matrix (day 0). After 2 days of culture (day 2), the spheres were treated with the indicated compound at final concentrations of 1, 2, and 5 μM. ( A ) Time course analysis of tumor spheroids growth, starting from 24 h after treatment (day 3), monitored by Incucyte ® S3 Live-Cell Analysis System (Essen BioScience, Ann Arbor, MI), 10× magnification. The results were expressed as the percentage of the spheroids area upon treatment relative to their own area before the compound administration. The histogram represents the mean values ± S.E.M. of at least four spheres. ( B ) Representative images of tumor spheroids growth described in panel (A). ( C ) Histological and immunohistochemical analysis of the spheroids generated from HCT116 cell lines and grown as in panel (A). Upper panels , representative images of immunostained sections. Lower panels, quantification of Ki-67, cleaved-caspase and γH2AX levels, expressed as percentage of positive cells. Thirty fields for condition were analyzed. The histograms represent the mean values ± S.D. of three independent experiments. * P < .05, ** P < .01, *** P < .001, and **** P < .0001.
Hct116 Brca2−/− Cell Derived Xenografts, supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cell lines hct116 brca2  (ATCC)
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ATCC cell lines hct116 brca2
Table 1
Cell Lines Hct116 Brca2, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hct116+brca2/HCT+116/pmc04882165-47-0-8
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cell lines hct116 brca2 - by Bioz Stars, 2026-09
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Antitumoral efficacy of NDI-NIs in advanced 3D models. Advanced 3D spheroids were established from human colorectal cancer cells (HCT116) by growing the cells within drops of a methylcellulose scaffold matrix (day 0). After 2 days of culture (day 2), the spheres were treated with the indicated compound at final concentrations of 1, 2, and 5 μM. ( A ) Time course analysis of tumor spheroids growth, starting from 24 h after treatment (day 3), monitored by Incucyte ® S3 Live-Cell Analysis System (Essen BioScience, Ann Arbor, MI), 10× magnification. The results were expressed as the percentage of the spheroids area upon treatment relative to their own area before the compound administration. The histogram represents the mean values ± S.E.M. of at least four spheres. ( B ) Representative images of tumor spheroids growth described in panel (A). ( C ) Histological and immunohistochemical analysis of the spheroids generated from HCT116 cell lines and grown as in panel (A). Upper panels , representative images of immunostained sections. Lower panels, quantification of Ki-67, cleaved-caspase and γH2AX levels, expressed as percentage of positive cells. Thirty fields for condition were analyzed. The histograms represent the mean values ± S.D. of three independent experiments. * P < .05, ** P < .01, *** P < .001, and **** P < .0001.

Journal: Nucleic Acids Research

Article Title: Naphthalene diimide-naphthalimide dyads promote telomere damage by selectively targeting multimeric G-quadruplexes

doi: 10.1093/nar/gkaf301

Figure Lengend Snippet: Antitumoral efficacy of NDI-NIs in advanced 3D models. Advanced 3D spheroids were established from human colorectal cancer cells (HCT116) by growing the cells within drops of a methylcellulose scaffold matrix (day 0). After 2 days of culture (day 2), the spheres were treated with the indicated compound at final concentrations of 1, 2, and 5 μM. ( A ) Time course analysis of tumor spheroids growth, starting from 24 h after treatment (day 3), monitored by Incucyte ® S3 Live-Cell Analysis System (Essen BioScience, Ann Arbor, MI), 10× magnification. The results were expressed as the percentage of the spheroids area upon treatment relative to their own area before the compound administration. The histogram represents the mean values ± S.E.M. of at least four spheres. ( B ) Representative images of tumor spheroids growth described in panel (A). ( C ) Histological and immunohistochemical analysis of the spheroids generated from HCT116 cell lines and grown as in panel (A). Upper panels , representative images of immunostained sections. Lower panels, quantification of Ki-67, cleaved-caspase and γH2AX levels, expressed as percentage of positive cells. Thirty fields for condition were analyzed. The histograms represent the mean values ± S.D. of three independent experiments. * P < .05, ** P < .01, *** P < .001, and **** P < .0001.

Article Snippet: HCT116 TP53 -/- were obtained by Dr Vogelstein, Johns Hopkins University, and HCT116 BRCA2 -/- were kindly gifted from Dr Madalena Tarsounas.

Techniques: Cell Analysis, Immunohistochemical staining, Generated

Table 1

Journal: The Journal of pathology

Article Title: Up-regulation of the interferon-related genes in BRCA2 knockout epithelial cells

doi: 10.1002/path.4404

Figure Lengend Snippet: Table 1

Article Snippet: Cell lines HCT116 BRCA2 +/+ cells were from ATCC (CCL-247), and the BRCA2 −/− cells were created in this study.

Techniques: