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(A) The expression of 3 xenantigens and 7 human transgenes in 10-GEC pig aortic endothelial cells (PAECs) was confirmed by flow cytometry. The levels of human IgG and IgM binding to 10-GE porcine PAECs. (C). The survival rate of 10-GEC porcine PBMCs. (D) <t>Coagulation</t> activity of 10-GEC pigs’ PAECs. (E) Phagocytosis of 10-GEC PAECs by human macrophages.
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(A) The expression of 3 xenantigens and 7 human transgenes in 10-GEC pig aortic endothelial cells (PAECs) was confirmed by flow cytometry. The levels of human IgG and IgM binding to 10-GE porcine PAECs. (C). The survival rate of 10-GEC porcine PBMCs. (D) <t>Coagulation</t> activity of 10-GEC pigs’ PAECs. (E) Phagocytosis of 10-GEC PAECs by human macrophages.
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(A) The expression of 3 xenantigens and 7 human transgenes in 10-GEC pig aortic endothelial cells (PAECs) was confirmed by flow cytometry. The levels of human IgG and IgM binding to 10-GE porcine PAECs. (C). The survival rate of 10-GEC porcine PBMCs. (D) <t>Coagulation</t> activity of 10-GEC pigs’ PAECs. (E) Phagocytosis of 10-GEC PAECs by human macrophages.
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(A) The expression of 3 xenantigens and 7 human transgenes in 10-GEC pig aortic endothelial cells (PAECs) was confirmed by flow cytometry. The levels of human IgG and IgM binding to 10-GE porcine PAECs. (C). The survival rate of 10-GEC porcine PBMCs. (D) Coagulation activity of 10-GEC pigs’ PAECs. (E) Phagocytosis of 10-GEC PAECs by human macrophages.

Journal: bioRxiv

Article Title: Specific Pathogen Free Ten Gene-Edited Pig Donor for Xenotransplantation

doi: 10.1101/2025.05.06.652168

Figure Lengend Snippet: (A) The expression of 3 xenantigens and 7 human transgenes in 10-GEC pig aortic endothelial cells (PAECs) was confirmed by flow cytometry. The levels of human IgG and IgM binding to 10-GE porcine PAECs. (C). The survival rate of 10-GEC porcine PBMCs. (D) Coagulation activity of 10-GEC pigs’ PAECs. (E) Phagocytosis of 10-GEC PAECs by human macrophages.

Article Snippet: The 10-GE donor pig developed by Revivicor knocked out 3 xenoantigens, 1 growth hormone receptor, 2 complement regulatory proteins (hCD46 and hCD55), 2 coagulation regulatory proteins (hTBM and hEPCR), 1 macrophage phagocytosis inhibiting gene (hCD47) and 1 anti-inflammation gene (hHO-1).

Techniques: Expressing, Flow Cytometry, Binding Assay, Coagulation, Activity Assay