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hbec 3kt cells  (ATCC)
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ATCC hbec 3kt cells
Inhibition of fucosylation <t>in</t> <t>HBEC-3KT</t> cells results in increased PT binding. A) Schematic of N-linked glycan synthesis inhibition in HBEC-3KT cells using small molecule inhibitors of glycosylation. 3FaxNeu5Ac and 2F-Fuc also impact other classes of glycoconjugates. HBEC-3KT cells were cultured in the presence of the inhibitors for 72 h. B) Flow cytometry analysis of PT binding to cell surfaces of the HBEC-3KT cells cultured with DMSO, 300 nM kifunensine, 50 μM 3F ax Neu5Ac, or 200 μM 2F-Fuc. Bar graph shows the quantification of geometric mean fluorescence from 3 independent trials, normalized to the geometric mean fluorescence of DMSO-treated cells. Statistical analyses were performed by one-way ANOVA (error bar indicates mean ± SD, * * * * indicates p value <0.0001). C) PT lectin blot of lysates of DMSO-, kifunensine-, 3F ax Neu5Ac-, or 2F-Fuc-treated HBEC-3KT cells. The blot shown is representative of 3 biological replicates.
Hbec 3kt Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hbec-3kt/HBEC3-KT/pmc13016894-134-0-21
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hbec 3kt cells - by Bioz Stars, 2026-10
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bronchial epithelial cell line hbec 3kt  (ATCC)
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ATCC bronchial epithelial cell line hbec 3kt
Inhibition of fucosylation <t>in</t> <t>HBEC-3KT</t> cells results in increased PT binding. A) Schematic of N-linked glycan synthesis inhibition in HBEC-3KT cells using small molecule inhibitors of glycosylation. 3FaxNeu5Ac and 2F-Fuc also impact other classes of glycoconjugates. HBEC-3KT cells were cultured in the presence of the inhibitors for 72 h. B) Flow cytometry analysis of PT binding to cell surfaces of the HBEC-3KT cells cultured with DMSO, 300 nM kifunensine, 50 μM 3F ax Neu5Ac, or 200 μM 2F-Fuc. Bar graph shows the quantification of geometric mean fluorescence from 3 independent trials, normalized to the geometric mean fluorescence of DMSO-treated cells. Statistical analyses were performed by one-way ANOVA (error bar indicates mean ± SD, * * * * indicates p value <0.0001). C) PT lectin blot of lysates of DMSO-, kifunensine-, 3F ax Neu5Ac-, or 2F-Fuc-treated HBEC-3KT cells. The blot shown is representative of 3 biological replicates.
Bronchial Epithelial Cell Line Hbec 3kt, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hbec-3kt/HBEC3-KT/pm41535907-81-13-22
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bronchial epithelial cell line hbec 3kt - by Bioz Stars, 2026-10
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human bronchial epithelial cell line hbec-3kt  (Johns Hopkins HealthCare)
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Johns Hopkins HealthCare human bronchial epithelial cell line hbec-3kt
Inhibition of fucosylation <t>in</t> <t>HBEC-3KT</t> cells results in increased PT binding. A) Schematic of N-linked glycan synthesis inhibition in HBEC-3KT cells using small molecule inhibitors of glycosylation. 3FaxNeu5Ac and 2F-Fuc also impact other classes of glycoconjugates. HBEC-3KT cells were cultured in the presence of the inhibitors for 72 h. B) Flow cytometry analysis of PT binding to cell surfaces of the HBEC-3KT cells cultured with DMSO, 300 nM kifunensine, 50 μM 3F ax Neu5Ac, or 200 μM 2F-Fuc. Bar graph shows the quantification of geometric mean fluorescence from 3 independent trials, normalized to the geometric mean fluorescence of DMSO-treated cells. Statistical analyses were performed by one-way ANOVA (error bar indicates mean ± SD, * * * * indicates p value <0.0001). C) PT lectin blot of lysates of DMSO-, kifunensine-, 3F ax Neu5Ac-, or 2F-Fuc-treated HBEC-3KT cells. The blot shown is representative of 3 biological replicates.
Human Bronchial Epithelial Cell Line Hbec 3kt, supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hbec-3kt/bronchial+epithelial+cells/pm40409044-78-2-16
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human bronchial epithelial cell line hbec-3kt - by Bioz Stars, 2026-10
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hbec-3kt  (Purdue University Cytometry)
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Purdue University Cytometry hbec-3kt
Inhibition of fucosylation <t>in</t> <t>HBEC-3KT</t> cells results in increased PT binding. A) Schematic of N-linked glycan synthesis inhibition in HBEC-3KT cells using small molecule inhibitors of glycosylation. 3FaxNeu5Ac and 2F-Fuc also impact other classes of glycoconjugates. HBEC-3KT cells were cultured in the presence of the inhibitors for 72 h. B) Flow cytometry analysis of PT binding to cell surfaces of the HBEC-3KT cells cultured with DMSO, 300 nM kifunensine, 50 μM 3F ax Neu5Ac, or 200 μM 2F-Fuc. Bar graph shows the quantification of geometric mean fluorescence from 3 independent trials, normalized to the geometric mean fluorescence of DMSO-treated cells. Statistical analyses were performed by one-way ANOVA (error bar indicates mean ± SD, * * * * indicates p value <0.0001). C) PT lectin blot of lysates of DMSO-, kifunensine-, 3F ax Neu5Ac-, or 2F-Fuc-treated HBEC-3KT cells. The blot shown is representative of 3 biological replicates.
Hbec 3kt, supplied by Purdue University Cytometry, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hbec-3kt/kb+cell+line/pmc11987007-51-0-6
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hbec-3kt cell line  (Purdue University Cytometry)
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Purdue University Cytometry hbec-3kt cell line
Inhibition of fucosylation <t>in</t> <t>HBEC-3KT</t> cells results in increased PT binding. A) Schematic of N-linked glycan synthesis inhibition in HBEC-3KT cells using small molecule inhibitors of glycosylation. 3FaxNeu5Ac and 2F-Fuc also impact other classes of glycoconjugates. HBEC-3KT cells were cultured in the presence of the inhibitors for 72 h. B) Flow cytometry analysis of PT binding to cell surfaces of the HBEC-3KT cells cultured with DMSO, 300 nM kifunensine, 50 μM 3F ax Neu5Ac, or 200 μM 2F-Fuc. Bar graph shows the quantification of geometric mean fluorescence from 3 independent trials, normalized to the geometric mean fluorescence of DMSO-treated cells. Statistical analyses were performed by one-way ANOVA (error bar indicates mean ± SD, * * * * indicates p value <0.0001). C) PT lectin blot of lysates of DMSO-, kifunensine-, 3F ax Neu5Ac-, or 2F-Fuc-treated HBEC-3KT cells. The blot shown is representative of 3 biological replicates.
Hbec 3kt Cell Line, supplied by Purdue University Cytometry, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hbec-3kt/kb+cell+line/10__1016_slash_j__isci__2025__112219-179-0-11
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hbec-3kt cell line - by Bioz Stars, 2026-10
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Inhibition of fucosylation in HBEC-3KT cells results in increased PT binding. A) Schematic of N-linked glycan synthesis inhibition in HBEC-3KT cells using small molecule inhibitors of glycosylation. 3FaxNeu5Ac and 2F-Fuc also impact other classes of glycoconjugates. HBEC-3KT cells were cultured in the presence of the inhibitors for 72 h. B) Flow cytometry analysis of PT binding to cell surfaces of the HBEC-3KT cells cultured with DMSO, 300 nM kifunensine, 50 μM 3F ax Neu5Ac, or 200 μM 2F-Fuc. Bar graph shows the quantification of geometric mean fluorescence from 3 independent trials, normalized to the geometric mean fluorescence of DMSO-treated cells. Statistical analyses were performed by one-way ANOVA (error bar indicates mean ± SD, * * * * indicates p value <0.0001). C) PT lectin blot of lysates of DMSO-, kifunensine-, 3F ax Neu5Ac-, or 2F-Fuc-treated HBEC-3KT cells. The blot shown is representative of 3 biological replicates.

Journal: Glycobiology

Article Title: Hypofucosylation promotes pertussis toxin binding to cell surface glycococonjugates and pertussis toxin-induced intracellular ERK signaling

doi: 10.1093/glycob/cwag011

Figure Lengend Snippet: Inhibition of fucosylation in HBEC-3KT cells results in increased PT binding. A) Schematic of N-linked glycan synthesis inhibition in HBEC-3KT cells using small molecule inhibitors of glycosylation. 3FaxNeu5Ac and 2F-Fuc also impact other classes of glycoconjugates. HBEC-3KT cells were cultured in the presence of the inhibitors for 72 h. B) Flow cytometry analysis of PT binding to cell surfaces of the HBEC-3KT cells cultured with DMSO, 300 nM kifunensine, 50 μM 3F ax Neu5Ac, or 200 μM 2F-Fuc. Bar graph shows the quantification of geometric mean fluorescence from 3 independent trials, normalized to the geometric mean fluorescence of DMSO-treated cells. Statistical analyses were performed by one-way ANOVA (error bar indicates mean ± SD, * * * * indicates p value <0.0001). C) PT lectin blot of lysates of DMSO-, kifunensine-, 3F ax Neu5Ac-, or 2F-Fuc-treated HBEC-3KT cells. The blot shown is representative of 3 biological replicates.

Article Snippet: HBEC-3KT cells were obtained from John Minna (UT Southwestern Medical Center). ( ) Colo205 and Jurkat cells were obtained from the ATCC.

Techniques: Inhibition, Binding Assay, Glycoproteomics, Cell Culture, Flow Cytometry, Fluorescence