Journal: bioRxiv

Article Title: Glycoprotein K8.1 is Critical for Liver and Bone Marrow Tropism of Kaposi’s Sarcoma-Associated Herpesvirus (KSHV) in the Marmoset Infection Model

doi: 10.1101/2024.10.31.621273

Figure Lengend Snippet: Immune cell populations after experimental infection with BAC16-derived KSHV wt, gH ASAELAAN or ΔK8.1. A) PD-1 expressing CD3 + cells. Significant changes for the KSHV wt group are highlighted by asterisks. B) PD-1 expressing CD4 + T cells. Significant changes for the KSHV wt group are highlighted by asterisks. C) PD-1 expressing CD8 + T cells. Significant changes for the KSHV wt group are highlighted by asterisks. D) PD-1 expressing CD20 + B cells. Significant changes for the KSHV wt group are highlighted by asterisks. E) CD21/CD27 double-positive CD20 + B cells. Significant changes for the KSHV gH ASELAAN group are highlighted by asterisks. F) CD21/CD27 double-negative CD20 + B cells. Significant changes for the KSHV wt group are highlighted by asterisks. Two-way ANOVA with Geisser-Greenhauser correction and Dunnett’s test, multiple comparison vs. baseline (week 0); * p<0.05, ** p<0.01, *** p<0.001.

Article Snippet: Used monoclonal antibodies included CD3-AlexaFluor700 (clone SP34-2), CD4-V450 (clone L200) from BD Biosciences (Heidelberg, Germany), CD8-APC-Cy7 (clone HIT8a), CD27 – Brilliant Violet650 (clone O323), CD45-Biotin (clone 6C9), CD80 – PE-Dazzle (clone 2D10), HLA-DR-PerCP-Cy5.5 (clone L243), PD-1-APC (clone EH12.2H7) from BioLegend (San Diego, USA), CD20 – PE (clone H299), CD159a – APC (clone Z199.1) from Beckman Coulter (Krefeld, Germany) as well as CD21-SuperBright600 (clone HB5) from eBioscience (Frankfurt am Main, Germany).

Techniques: Infection, Derivative Assay, Expressing, Comparison

Journal: Biochemistry and Biophysics Reports

Article Title: The fixation of complement protein pairs to CR2 isoforms

doi: 10.1016/j.bbrep.2024.101657

Figure Lengend Snippet: Raji cells were treated with PBS (lane 1), i-C3 (lane 2; 1,9 μg), i-C3 (lane 3; 7,6 μg), hi-i-C3 (lane 4; 7,6 μg), OKB7 (lanes 5, 6), HB5 (lanes 7, 8) for 30 min at 37 °C. The washed cells were treated with PBS (lanes 1–5, 7) or i-C3 (lanes 6, 8; 7,6 μg) for further 20 min at 37 °C. The washed cells were lysed and run under reducing condition on 7.5 % SDS-PAGE. The run proteins were blotted on nitrocellulose membrane and incubated with anti-iC3b MoAbs (1:200). Immunoblot was developed with anti-mouse peroxidase.

Article Snippet: Mouse anti-CR2 MoAb, OKB7, from Ortho Pharmaceutical Corp. (Raritan, NJ, USA), anti-CR2 MoAb, HB5, from Becton Dickinson (Mountain View, CA, USA).

Techniques: SDS Page, Membrane, Incubation, Western Blot

Journal: Biochemistry and Biophysics Reports

Article Title: The fixation of complement protein pairs to CR2 isoforms

doi: 10.1016/j.bbrep.2024.101657

Figure Lengend Snippet: Panel A: Raji cells were treated with PBS (lane 1), i-C3 (lane 2), OKB7 (lane 3), with OKB7 first and immediately afterwards with i-C3 (7,6 μg) (lane 4), HB5 (lane 5), with HB5 first and immediately afterwards with i-C3 (7,6 μg) (lane 6) for 30 min at 37 °C. The washed cells were lysed and run under reducing condition on 7.5 % SDS-PAGE. The run proteins were blotted on nitrocellulose membrane and incubated with anti-iC3b MoAbs (1:200). Immunoblot was developed with anti-mouse peroxidase. Panel B, lanes 2, 4, 6: the same as described in panel A but obtained from a film exposed for a shorter time. Panels C, D, lanes 2, 4, 6: the same as described in panel A but obtained varying brightness and contrast of a film exposed for a shorter time.

Article Snippet: Mouse anti-CR2 MoAb, OKB7, from Ortho Pharmaceutical Corp. (Raritan, NJ, USA), anti-CR2 MoAb, HB5, from Becton Dickinson (Mountain View, CA, USA).

Techniques: SDS Page, Membrane, Incubation, Western Blot

Journal: Biochemistry and Biophysics Reports

Article Title: The fixation of complement protein pairs to CR2 isoforms

doi: 10.1016/j.bbrep.2024.101657

Figure Lengend Snippet: Raji cells were treated PBS, OKB7 or HB5 for 30 min at 4 °C. The supernatant of PBS- (lanes 1, 7), OKB7- (lanes 3, 8) and HB5-treated cells (lane 5) and the cell lysate of PBS- (lanes 2, 9), OKB7- (lanes 4, 10) and of HB5-treated cells (lane 6) were lysed under non reducing condition and run on 7.5 SDS-PAGE. The proteins were blotted on nitrocellulose membrane and incubated with anti-C3d polyclonal Ab 1:500 (lanes 1–6) or PBS (lanes 7–10). Immunoblot was developed with anti-rabbit peroxidase.

Article Snippet: Mouse anti-CR2 MoAb, OKB7, from Ortho Pharmaceutical Corp. (Raritan, NJ, USA), anti-CR2 MoAb, HB5, from Becton Dickinson (Mountain View, CA, USA).

Techniques: SDS Page, Membrane, Incubation, Western Blot

Journal: Biochemistry and Biophysics Reports

Article Title: The fixation of complement protein pairs to CR2 isoforms

doi: 10.1016/j.bbrep.2024.101657

Figure Lengend Snippet: Panel A, Raji cell supernatant was treated with Laemmli's buffer and run on 7.5 % SDS-PAGE under non reducing (lanes 1, 3) or reducing condition (lanes 2, 4). The proteins blotted on nitrocellulose membrane were incubated with OKB7 (lanes 1, 2) or HB5 (lanes 3, 4). Immunoblot was developed with anti-mouse peroxidase. Panel B, lanes 1, 2, 3, 4: the same as described in panel A but using the same supernatant that has been stored at −20 °C for 6 days and referred as F/T supernatant.

Article Snippet: Mouse anti-CR2 MoAb, OKB7, from Ortho Pharmaceutical Corp. (Raritan, NJ, USA), anti-CR2 MoAb, HB5, from Becton Dickinson (Mountain View, CA, USA).

Techniques: SDS Page, Membrane, Incubation, Western Blot

Journal: iScience

Article Title: Atypical B cells consist of subsets with distinct functional profiles

doi: 10.1016/j.isci.2023.108496

Figure Lengend Snippet: Phenotypically defined atypical B cells (CD21 − CD27 − IgG + CD11c + ) are found in three transcriptionally distinct clusters (A) Distribution of CD21 −/+ CD27 −/+ B cells (based on surface marker expression) among all transcriptional clusters. Clusters 3, 5, and 6 (indicated with blue arrow heads) contain atypical B cells. (B) Distribution of CD11c −/+ CXCR3 −/+ B cells (based on surface marker expression) among all transcriptional clusters. (C) Distribution of immunoglobulin heavy-chain ( IGH ) transcripts among all transcriptional clusters. (D) Projection of phenotypically defined atypical B cells (shown in red) onto the transcriptomics-based UMAP. All non-atypical B cells are shown in gray. (E) Distribution of each B cell population over the different clusters, shown as a percentage of that population (left), and the percentage of phenotypically defined B cell populations within each cluster (right). The direction in which the values in the rows (left) or columns (right) add up to 100% is indicated with an arrow. Rest., resting; act., activated; NAV, naive, unswM, unswitched memory; swM, switched memory; DN, double-negative. See also Figures S5–S10 .

Article Snippet: PerCP-eFluor 710 anti-human CD21 (clone HB5) , Thermo , Cat# 46-0219-42; RRID: AB_2573672.

Techniques: Marker, Expressing

Journal: iScience

Article Title: Atypical B cells consist of subsets with distinct functional profiles

doi: 10.1016/j.isci.2023.108496

Figure Lengend Snippet:

Article Snippet: PerCP-eFluor 710 anti-human CD21 (clone HB5) , Thermo , Cat# 46-0219-42; RRID: AB_2573672.

Techniques: Staining, Recombinant, Cell Isolation, Multiplex Assay, Sequencing, Plasmid Preparation, Software