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High-throughput automated transformation workflow for Cyanidioschyzon merolae . Overview of the fully automated transformation and screening pipeline integrating an OT-2 liquid-handling robot and a PIXL colony-picking system. Wild-type cultures are grown to mid-log phase, and DNA constructs are prepared for PEG-mediated transformation. The OT-2 performs transformation. After cell recovery, automated serial dilution and plating on starch-bed MA2G plates using Z-offset calibration based on gellan-gum weigh. After colony formation, the PIXL robot identifies and transfers individual colonies into 96-well plates for propagation. Colonies are subsequently arrayed in starch bed plates, ready for colony PCR setup on the OT-2.

Journal: bioRxiv

Article Title: Genome Position Does Not Impact Transgene Expression Efficiency in the Ancient Red Alga Cyanidioschyzon merolae

doi: 10.64898/2026.01.31.703004

Figure Lengend Snippet: High-throughput automated transformation workflow for Cyanidioschyzon merolae . Overview of the fully automated transformation and screening pipeline integrating an OT-2 liquid-handling robot and a PIXL colony-picking system. Wild-type cultures are grown to mid-log phase, and DNA constructs are prepared for PEG-mediated transformation. The OT-2 performs transformation. After cell recovery, automated serial dilution and plating on starch-bed MA2G plates using Z-offset calibration based on gellan-gum weigh. After colony formation, the PIXL robot identifies and transfers individual colonies into 96-well plates for propagation. Colonies are subsequently arrayed in starch bed plates, ready for colony PCR setup on the OT-2.

Article Snippet: The OT-2 hardware configuration included a multichannel P300 and a multichannel P20 Gen 2 pipette, Gen 1 Heater-Shaker Module, Gen 1 Temperature Module, Deep-Well Metal Adapter, and standard Opentrons P20 and P300 tip racks all from Opentrons, USA.

Techniques: High Throughput Screening Assay, Transformation Assay, Construct, Cell Recovery, Serial Dilution, Starch