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cd81  (Bio-Rad)


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    Structured Review

    Bio-Rad cd81
    The diameter and concentration of particles present in EV preparation were determined by nanoparticle analysis and immunoblotting with EV-specific markers. EV concentration by particle diameter was obtained from Nanosight (A) after isolation from R-28 cells shown as magnified at 10×, Scale bar: 50 µm (B). EVs were enriched for the EV marker <t>CD81</t> compared to R-28 cell lysates when the same density of protein was loaded (C). Over three separate isolations, the average concentration (D) and size of the EVs (E) were determined by Nanosight ( n = 3). Electron microscopic imaging of the R-28 cells with EVs on the cell surface is visible (inset; F), and EVs were also detected in the purified EV preparation (G). EV: Extracellular vesicle.
    Cd81, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd81/product/Bio-Rad
    Average 93 stars, based on 21 article reviews
    cd81 - by Bioz Stars, 2026-02
    93/100 stars

    Images

    1) Product Images from "R-28 cell-derived extracellular vesicles protect retinal ganglion cells in glaucoma"

    Article Title: R-28 cell-derived extracellular vesicles protect retinal ganglion cells in glaucoma

    Journal: Neural Regeneration Research

    doi: 10.4103/NRR.NRR-D-24-00709

    The diameter and concentration of particles present in EV preparation were determined by nanoparticle analysis and immunoblotting with EV-specific markers. EV concentration by particle diameter was obtained from Nanosight (A) after isolation from R-28 cells shown as magnified at 10×, Scale bar: 50 µm (B). EVs were enriched for the EV marker CD81 compared to R-28 cell lysates when the same density of protein was loaded (C). Over three separate isolations, the average concentration (D) and size of the EVs (E) were determined by Nanosight ( n = 3). Electron microscopic imaging of the R-28 cells with EVs on the cell surface is visible (inset; F), and EVs were also detected in the purified EV preparation (G). EV: Extracellular vesicle.
    Figure Legend Snippet: The diameter and concentration of particles present in EV preparation were determined by nanoparticle analysis and immunoblotting with EV-specific markers. EV concentration by particle diameter was obtained from Nanosight (A) after isolation from R-28 cells shown as magnified at 10×, Scale bar: 50 µm (B). EVs were enriched for the EV marker CD81 compared to R-28 cell lysates when the same density of protein was loaded (C). Over three separate isolations, the average concentration (D) and size of the EVs (E) were determined by Nanosight ( n = 3). Electron microscopic imaging of the R-28 cells with EVs on the cell surface is visible (inset; F), and EVs were also detected in the purified EV preparation (G). EV: Extracellular vesicle.

    Techniques Used: Concentration Assay, Western Blot, Isolation, Marker, Imaging, Purification



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    The diameter and concentration of particles present in EV preparation were determined by nanoparticle analysis and immunoblotting with EV-specific markers. EV concentration by particle diameter was obtained from Nanosight (A) after isolation from R-28 cells shown as magnified at 10×, Scale bar: 50 µm (B). EVs were enriched for the EV marker <t>CD81</t> compared to R-28 cell lysates when the same density of protein was loaded (C). Over three separate isolations, the average concentration (D) and size of the EVs (E) were determined by Nanosight ( n = 3). Electron microscopic imaging of the R-28 cells with EVs on the cell surface is visible (inset; F), and EVs were also detected in the purified EV preparation (G). EV: Extracellular vesicle.
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    The diameter and concentration of particles present in EV preparation were determined by nanoparticle analysis and immunoblotting with EV-specific markers. EV concentration by particle diameter was obtained from Nanosight (A) after isolation from R-28 cells shown as magnified at 10×, Scale bar: 50 µm (B). EVs were enriched for the EV marker <t>CD81</t> compared to R-28 cell lysates when the same density of protein was loaded (C). Over three separate isolations, the average concentration (D) and size of the EVs (E) were determined by Nanosight ( n = 3). Electron microscopic imaging of the R-28 cells with EVs on the cell surface is visible (inset; F), and EVs were also detected in the purified EV preparation (G). EV: Extracellular vesicle.
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    The diameter and concentration of particles present in EV preparation were determined by nanoparticle analysis and immunoblotting with EV-specific markers. EV concentration by particle diameter was obtained from Nanosight (A) after isolation from R-28 cells shown as magnified at 10×, Scale bar: 50 µm (B). EVs were enriched for the EV marker <t>CD81</t> compared to R-28 cell lysates when the same density of protein was loaded (C). Over three separate isolations, the average concentration (D) and size of the EVs (E) were determined by Nanosight ( n = 3). Electron microscopic imaging of the R-28 cells with EVs on the cell surface is visible (inset; F), and EVs were also detected in the purified EV preparation (G). EV: Extracellular vesicle.
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    The diameter and concentration of particles present in EV preparation were determined by nanoparticle analysis and immunoblotting with EV-specific markers. EV concentration by particle diameter was obtained from Nanosight (A) after isolation from R-28 cells shown as magnified at 10×, Scale bar: 50 µm (B). EVs were enriched for the EV marker <t>CD81</t> compared to R-28 cell lysates when the same density of protein was loaded (C). Over three separate isolations, the average concentration (D) and size of the EVs (E) were determined by Nanosight ( n = 3). Electron microscopic imaging of the R-28 cells with EVs on the cell surface is visible (inset; F), and EVs were also detected in the purified EV preparation (G). EV: Extracellular vesicle.
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    Image Search Results


    The diameter and concentration of particles present in EV preparation were determined by nanoparticle analysis and immunoblotting with EV-specific markers. EV concentration by particle diameter was obtained from Nanosight (A) after isolation from R-28 cells shown as magnified at 10×, Scale bar: 50 µm (B). EVs were enriched for the EV marker CD81 compared to R-28 cell lysates when the same density of protein was loaded (C). Over three separate isolations, the average concentration (D) and size of the EVs (E) were determined by Nanosight ( n = 3). Electron microscopic imaging of the R-28 cells with EVs on the cell surface is visible (inset; F), and EVs were also detected in the purified EV preparation (G). EV: Extracellular vesicle.

    Journal: Neural Regeneration Research

    Article Title: R-28 cell-derived extracellular vesicles protect retinal ganglion cells in glaucoma

    doi: 10.4103/NRR.NRR-D-24-00709

    Figure Lengend Snippet: The diameter and concentration of particles present in EV preparation were determined by nanoparticle analysis and immunoblotting with EV-specific markers. EV concentration by particle diameter was obtained from Nanosight (A) after isolation from R-28 cells shown as magnified at 10×, Scale bar: 50 µm (B). EVs were enriched for the EV marker CD81 compared to R-28 cell lysates when the same density of protein was loaded (C). Over three separate isolations, the average concentration (D) and size of the EVs (E) were determined by Nanosight ( n = 3). Electron microscopic imaging of the R-28 cells with EVs on the cell surface is visible (inset; F), and EVs were also detected in the purified EV preparation (G). EV: Extracellular vesicle.

    Article Snippet: CD81 , Hamster , BioRad, Watford, UK , 1:100 , MCA1846 , N/A.

    Techniques: Concentration Assay, Western Blot, Isolation, Marker, Imaging, Purification