Journal: Cell Stress & Chaperones

Article Title: Hypoxia-induced GRP78 activation disrupts the Fndc5/Irisin axis to accelerate skeletal muscle atrophy

doi: 10.1016/j.cstres.2026.100176

Figure Lengend Snippet: Effects of HA15 on C2C12 myoblast viability, GRP78 and Fndc5/irisin expression, and myotube formation. (a) CCK-8 assay showing the viability of C2C12 myoblasts treated with different concentrations of HA15 (1, 5, 10, 50, and 100 µM). Data are presented as mean ± SD ( n = 6), with significant differences indicated ( P < 0.05). (b) Western blot analysis of GRP78 and Fndc5/irisin protein levels in C2C12 myoblasts after treatment with HA15 at the indicated concentrations. β-Tubulin is used as a loading control. (c) Quantification of GRP78 protein expression normalized to β-Tubulin from the Western blot. Data are presented as mean ± SD ( n = 3), with significant differences indicated ( P < 0.001). (d) Immunofluorescence staining showing the expression of MyHC (green) and nuclear staining with Hoechst (blue) in C2C12 myotubes treated with various concentrations of HA15 (1 µM, 5 µM, 10 µM, 50 µM, and 100 µM). (e) Quantification of the myotube fusion index, data presented as mean ± SD ( n = 3). (f) Quantification of myotube diameter, data presented as mean ± SD ( n = 3). (g) Quantification of myotube area, data presented as mean ± SD ( n = 3). Significant differences are indicated by P values.

Article Snippet: The impact of HA15 on cell viability was assessed using the Cell Counting Kit-8 (CCK-8; TargetMol, Boston, MA, USA; C0005).

Techniques: Expressing, CCK-8 Assay, Western Blot, Control, Immunofluorescence, Staining

Journal: Bioactive Materials

Article Title: Multi-omics-informed hydrogel design: modulating IL-6 to reduce endoplasmic reticulum stress in bone regeneration

doi: 10.1016/j.bioactmat.2025.09.005

Figure Lengend Snippet: IL-6-mediated regulation of HSPA5 in early bone defect repair tissues. ( A ) Extraction of femoral and alveolar bone cell clusters from whole-cell atlases. ( B ) Pseudo-time series analysis of cellular and gene expression in repair tissues. ( C ) The rat model for femoral and alveolar bone defect treatment using HA15 and LMT28. ( D , E ) Immunohistochemical analysis of HSPA5 and IL-6 expression in femoral defects after HA15 treatment. Scale bars: 200 μm and 50 μm. ( F , G ) Immunohistochemical analysis in alveolar defects after LMT28 treatment. Scale bars: 200 μm and 50 μm. HA15 inhibits HSPA5; LMT28 inhibits IL-6. Statistical significance was assessed with a t -test.

Article Snippet: Following the femoral defect creation, HA15 (HY-100437, MCE, US), LMT28 (HY-102084, MCE, US), HM03 (HY-125974, MCE, US), and SC144 (HY-15614, MCE, US) were administered to the rats via intraperitoneal injection, in accordance with the manufacturer's instructions.

Techniques: Extraction, Gene Expression, Immunohistochemical staining, Expressing

Journal: Bioactive Materials

Article Title: Multi-omics-informed hydrogel design: modulating IL-6 to reduce endoplasmic reticulum stress in bone regeneration

doi: 10.1016/j.bioactmat.2025.09.005

Figure Lengend Snippet: IL-6 modulates HSPA5 to mitigate endoplasmic reticulum stress (ERS)-related apoptosis in early bone defects. ( A , B ) Expression and quantitative analysis of ERS-related proteins following HA15 and LMT28 treatments. Scale bars: 200 μm and 50 μm. ( C , D ) Quantitative analysis of apoptosis-related proteins CHOP and caspase-12 after treatment. Scale bars: 200 μm and 50 μm. ( E ) Expression levels of ERS and apoptosis-related proteins were analyzed by Western blot, and statistical analysis was conducted on the results. ( F , G ) Quantitative terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining and statistical analysis of apoptosis in femoral and alveolar bone defects. Scale bars: 100 μm. ( H ) Ca 2+ histopathological staining. Scale bars: 100 μm and 40 μm. ( I ) Detection of reactive oxygen species in repair tissue following HA15 and LMT28 treatment. Scale bars: 200 μm and 50 μm. Statistical significance was determined using one-way ANOVA.

Article Snippet: Following the femoral defect creation, HA15 (HY-100437, MCE, US), LMT28 (HY-102084, MCE, US), HM03 (HY-125974, MCE, US), and SC144 (HY-15614, MCE, US) were administered to the rats via intraperitoneal injection, in accordance with the manufacturer's instructions.

Techniques: Expressing, Western Blot, TUNEL Assay, Staining

Journal: Translational Oncology

Article Title: Overcoming the leptomeningeal seeding of medulloblastoma by targeting HSP70

doi: 10.1016/j.tranon.2026.102695

Figure Lengend Snippet: In vitro and in vivo evaluation of combination therapy targeting HSP70 in LMS model. (A, E) Dose-response curves for single and combination treatments of VER155008 with cisplatin (A) or 4HC (E) in S3 cells. The leftward shift of the combination curve (pink line) compared to monotherapy (black line) indicates enhanced efficacy. (B, F) Combination Index (CI) vs. fraction affected (Fa) plots for VER155008 + cisplatin combination (B) and VER155008 + 4HC combination (F), generated by CompuSyn software. CI values below 1 indicate synergy. (C, G) Isobologram analysis for VER155008 + cisplatin combination (C) and VER155008 + 4HC combination (G), showing dose pairs required to achieve 50% (Fa = 0.5, blue), 75% (Fa = 0.75, red), and 90% (Fa = 0.9, green) growth inhibition. (D) Cell viability comparison between single-agent and combination treatments of VER155008 with cisplatin in S3 cells. (H) Cell viability comparison between single-agent and combination treatments of VER155008 with 4HC in S3 cells. The combination showed significantly greater inhibitory effect than monotherapy. (I) Representative bioluminescence imaging (BLI) of LMS-bearing mice treated with vehicle, VER155008 alone, or in combination with cisplatin or ifosfamide (IFO) at days 1, 10, and 17. (J) Longitudinal quantification of BLI signal intensity in the brain region of interest (ROI) over time for each treatment group. (K) Longitudinal quantification of BLI signal intensity in the spinal cord region of interest (ROI) over time for each treatment group. Quantification of photon flux was performed for brain and spinal cord regions of interest (ROI) at the indicated time points. In vivo data are presented as mean ± SD, with n = 5 mice per group. Statistical analysis was conducted using repeated-measures ANOVA followed by post hoc testing. *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: VER155008, Apoptozole, HA15, TRC051384, JG98 , Elesclomol, cisplatin (Selleckchem, Houston, TX.

Techniques: In Vitro, In Vivo, Generated, Software, Inhibition, Comparison, Imaging