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Image Search Results


A, WT-hSERT HEK-293 cells and HA-hSERT-LLC-PK 1 cells underwent ABE followed by SDS-PAGE and immunoblotting to determine SERT palmitoylation levels (ABE, top panels) and total SERT levels (bottom panels). Extended black lines (|) on the top and bottom of panel A indicate two separate blots/experiments while black lines within the boundaries of the image indicate the removal of duplicate lanes or rearrangement of lane images from the same blot. M r markers for all blots are shown at right. The validated primary monoclonal antibodies used for immunoblotting were anti-human serotonin transporter (ST51-2) for HEK-293 cells expressing WT-hSERT, and anti-hemagglutinin (Anti-HA) for HA-hSERT detection. WT hSERT HEK-293 cells (B) or WT HA-hSERT LLC-PK 1 cells (D) underwent ABE after treatment with vehicle or 10 µM 2BP for 18 hours and palmitoylation levels were determined after SDS-PAGE by immunoblotting. C, Quantification of hSERT palmitoylation in C (mean ± SD of 3 independent experiments relative to control normalized to 100%.; ** p < 0.01 versus control; Student’s t-test for independent samples). E, Quantification of palmitoylation in D (mean ± SD of 5 independent experiments relative to control normalized to 100%; ** p < 0.01 versus control; Student’s t-test for independent samples).

Journal: bioRxiv

Article Title: Palmitoylation regulates human serotonin transporter activity, trafficking, and expression and is modulated by escitalopram

doi: 10.1101/2023.05.09.540092

Figure Lengend Snippet: A, WT-hSERT HEK-293 cells and HA-hSERT-LLC-PK 1 cells underwent ABE followed by SDS-PAGE and immunoblotting to determine SERT palmitoylation levels (ABE, top panels) and total SERT levels (bottom panels). Extended black lines (|) on the top and bottom of panel A indicate two separate blots/experiments while black lines within the boundaries of the image indicate the removal of duplicate lanes or rearrangement of lane images from the same blot. M r markers for all blots are shown at right. The validated primary monoclonal antibodies used for immunoblotting were anti-human serotonin transporter (ST51-2) for HEK-293 cells expressing WT-hSERT, and anti-hemagglutinin (Anti-HA) for HA-hSERT detection. WT hSERT HEK-293 cells (B) or WT HA-hSERT LLC-PK 1 cells (D) underwent ABE after treatment with vehicle or 10 µM 2BP for 18 hours and palmitoylation levels were determined after SDS-PAGE by immunoblotting. C, Quantification of hSERT palmitoylation in C (mean ± SD of 3 independent experiments relative to control normalized to 100%.; ** p < 0.01 versus control; Student’s t-test for independent samples). E, Quantification of palmitoylation in D (mean ± SD of 5 independent experiments relative to control normalized to 100%; ** p < 0.01 versus control; Student’s t-test for independent samples).

Article Snippet: SERT expression level was verified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting of the cellular lysates against Anti-HA (Biolegends) and anti-human (h) SERT (Mabtechnologies-ST51-2) specific antibodies.

Techniques: SDS Page, Western Blot, Expressing