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anti gst biosensors  (Gator Bio Inc)
93
Gator Bio Inc anti gst biosensors
(A) MKK5-GFP was affinity purified from 7-day-old WT but not spy-4 seedlings using Aleuria Aurantia Lectin (AAL) immobilized on agarose beads. Coomassie Brilliant Blue staining (CBB) of the membrane shows the loading control. (B) Bio-layer interferometry (BLI) assay showing binding kinetics between SPY and MKK4. 6xHis-SUMO-3TPR-SPY was immobilized to anti-His sensors and dipped into indicated concentrations of GST-MKK4 protein. The dissociation constant (K d ) was calculated by steady-state fitting of GST-MKK4 concentration against the response unit. (C) Co-immunoprecipitation shows in vivo interaction between SPY and MKK5. YFP or SPY-YFP was transiently co-expressed with MKK5-myc in N. benthamiana leaves, immunoprecipitated by anti-GFP antibody, and immunoblotted with anti-GFP or anti-myc antibody. (D) in vitro affinity purification (pull-down) assay shows direct interaction between SPY/SEC and MKK4/5. Recombinant GST, GST-MKK4, and GST-MKK5 were incubated with 6xHis-tagged SPY, SEC, and PCK1, affinity-purified by Glutathione-agarose beads, and immunoblotted using anti-His <t>and</t> <t>anti-GST</t> antibodies. (E) Higher energy collisional dissociation (HCD) mass spectrum shows O-fucosylation on the activation loop peptide of MKK4 spanning amino acids 220-242, after in vitro O-fucosylation by SPY.
Anti Gst Biosensors, supplied by Gator Bio Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti gst biosensors/product/Gator Bio Inc
Average 93 stars, based on 1 article reviews
anti gst biosensors - by Bioz Stars, 2026-05
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gst  (Kaneka Corp)
86
Kaneka Corp gst
(A) MKK5-GFP was affinity purified from 7-day-old WT but not spy-4 seedlings using Aleuria Aurantia Lectin (AAL) immobilized on agarose beads. Coomassie Brilliant Blue staining (CBB) of the membrane shows the loading control. (B) Bio-layer interferometry (BLI) assay showing binding kinetics between SPY and MKK4. 6xHis-SUMO-3TPR-SPY was immobilized to anti-His sensors and dipped into indicated concentrations of GST-MKK4 protein. The dissociation constant (K d ) was calculated by steady-state fitting of GST-MKK4 concentration against the response unit. (C) Co-immunoprecipitation shows in vivo interaction between SPY and MKK5. YFP or SPY-YFP was transiently co-expressed with MKK5-myc in N. benthamiana leaves, immunoprecipitated by anti-GFP antibody, and immunoblotted with anti-GFP or anti-myc antibody. (D) in vitro affinity purification (pull-down) assay shows direct interaction between SPY/SEC and MKK4/5. Recombinant GST, GST-MKK4, and GST-MKK5 were incubated with 6xHis-tagged SPY, SEC, and PCK1, affinity-purified by Glutathione-agarose beads, and immunoblotted using anti-His <t>and</t> <t>anti-GST</t> antibodies. (E) Higher energy collisional dissociation (HCD) mass spectrum shows O-fucosylation on the activation loop peptide of MKK4 spanning amino acids 220-242, after in vitro O-fucosylation by SPY.
Gst, supplied by Kaneka Corp, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gst/product/Kaneka Corp
Average 86 stars, based on 1 article reviews
gst - by Bioz Stars, 2026-05
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colorimetric gst assay kit  (Nanjing Jiancheng Bioengineering Research Institute Co Ltd)
86
Nanjing Jiancheng Bioengineering Research Institute Co Ltd colorimetric gst assay kit
(A) MKK5-GFP was affinity purified from 7-day-old WT but not spy-4 seedlings using Aleuria Aurantia Lectin (AAL) immobilized on agarose beads. Coomassie Brilliant Blue staining (CBB) of the membrane shows the loading control. (B) Bio-layer interferometry (BLI) assay showing binding kinetics between SPY and MKK4. 6xHis-SUMO-3TPR-SPY was immobilized to anti-His sensors and dipped into indicated concentrations of GST-MKK4 protein. The dissociation constant (K d ) was calculated by steady-state fitting of GST-MKK4 concentration against the response unit. (C) Co-immunoprecipitation shows in vivo interaction between SPY and MKK5. YFP or SPY-YFP was transiently co-expressed with MKK5-myc in N. benthamiana leaves, immunoprecipitated by anti-GFP antibody, and immunoblotted with anti-GFP or anti-myc antibody. (D) in vitro affinity purification (pull-down) assay shows direct interaction between SPY/SEC and MKK4/5. Recombinant GST, GST-MKK4, and GST-MKK5 were incubated with 6xHis-tagged SPY, SEC, and PCK1, affinity-purified by Glutathione-agarose beads, and immunoblotted using anti-His <t>and</t> <t>anti-GST</t> antibodies. (E) Higher energy collisional dissociation (HCD) mass spectrum shows O-fucosylation on the activation loop peptide of MKK4 spanning amino acids 220-242, after in vitro O-fucosylation by SPY.
Colorimetric Gst Assay Kit, supplied by Nanjing Jiancheng Bioengineering Research Institute Co Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/colorimetric gst assay kit/product/Nanjing Jiancheng Bioengineering Research Institute Co Ltd
Average 86 stars, based on 1 article reviews
colorimetric gst assay kit - by Bioz Stars, 2026-05
86/100 stars
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gst  (Jiancheng Inc)
86
Jiancheng Inc gst
(A) MKK5-GFP was affinity purified from 7-day-old WT but not spy-4 seedlings using Aleuria Aurantia Lectin (AAL) immobilized on agarose beads. Coomassie Brilliant Blue staining (CBB) of the membrane shows the loading control. (B) Bio-layer interferometry (BLI) assay showing binding kinetics between SPY and MKK4. 6xHis-SUMO-3TPR-SPY was immobilized to anti-His sensors and dipped into indicated concentrations of GST-MKK4 protein. The dissociation constant (K d ) was calculated by steady-state fitting of GST-MKK4 concentration against the response unit. (C) Co-immunoprecipitation shows in vivo interaction between SPY and MKK5. YFP or SPY-YFP was transiently co-expressed with MKK5-myc in N. benthamiana leaves, immunoprecipitated by anti-GFP antibody, and immunoblotted with anti-GFP or anti-myc antibody. (D) in vitro affinity purification (pull-down) assay shows direct interaction between SPY/SEC and MKK4/5. Recombinant GST, GST-MKK4, and GST-MKK5 were incubated with 6xHis-tagged SPY, SEC, and PCK1, affinity-purified by Glutathione-agarose beads, and immunoblotted using anti-His <t>and</t> <t>anti-GST</t> antibodies. (E) Higher energy collisional dissociation (HCD) mass spectrum shows O-fucosylation on the activation loop peptide of MKK4 spanning amino acids 220-242, after in vitro O-fucosylation by SPY.
Gst, supplied by Jiancheng Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gst/product/Jiancheng Inc
Average 86 stars, based on 1 article reviews
gst - by Bioz Stars, 2026-05
86/100 stars
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anti gst  (Abmart Inc)
86
Abmart Inc anti gst
(A) MKK5-GFP was affinity purified from 7-day-old WT but not spy-4 seedlings using Aleuria Aurantia Lectin (AAL) immobilized on agarose beads. Coomassie Brilliant Blue staining (CBB) of the membrane shows the loading control. (B) Bio-layer interferometry (BLI) assay showing binding kinetics between SPY and MKK4. 6xHis-SUMO-3TPR-SPY was immobilized to anti-His sensors and dipped into indicated concentrations of GST-MKK4 protein. The dissociation constant (K d ) was calculated by steady-state fitting of GST-MKK4 concentration against the response unit. (C) Co-immunoprecipitation shows in vivo interaction between SPY and MKK5. YFP or SPY-YFP was transiently co-expressed with MKK5-myc in N. benthamiana leaves, immunoprecipitated by anti-GFP antibody, and immunoblotted with anti-GFP or anti-myc antibody. (D) in vitro affinity purification (pull-down) assay shows direct interaction between SPY/SEC and MKK4/5. Recombinant GST, GST-MKK4, and GST-MKK5 were incubated with 6xHis-tagged SPY, SEC, and PCK1, affinity-purified by Glutathione-agarose beads, and immunoblotted using anti-His <t>and</t> <t>anti-GST</t> antibodies. (E) Higher energy collisional dissociation (HCD) mass spectrum shows O-fucosylation on the activation loop peptide of MKK4 spanning amino acids 220-242, after in vitro O-fucosylation by SPY.
Anti Gst, supplied by Abmart Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti gst/product/Abmart Inc
Average 86 stars, based on 1 article reviews
anti gst - by Bioz Stars, 2026-05
86/100 stars
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anti gst antibody  (Abmart Inc)
86
Abmart Inc anti gst antibody
(A) MKK5-GFP was affinity purified from 7-day-old WT but not spy-4 seedlings using Aleuria Aurantia Lectin (AAL) immobilized on agarose beads. Coomassie Brilliant Blue staining (CBB) of the membrane shows the loading control. (B) Bio-layer interferometry (BLI) assay showing binding kinetics between SPY and MKK4. 6xHis-SUMO-3TPR-SPY was immobilized to anti-His sensors and dipped into indicated concentrations of GST-MKK4 protein. The dissociation constant (K d ) was calculated by steady-state fitting of GST-MKK4 concentration against the response unit. (C) Co-immunoprecipitation shows in vivo interaction between SPY and MKK5. YFP or SPY-YFP was transiently co-expressed with MKK5-myc in N. benthamiana leaves, immunoprecipitated by anti-GFP antibody, and immunoblotted with anti-GFP or anti-myc antibody. (D) in vitro affinity purification (pull-down) assay shows direct interaction between SPY/SEC and MKK4/5. Recombinant GST, GST-MKK4, and GST-MKK5 were incubated with 6xHis-tagged SPY, SEC, and PCK1, affinity-purified by Glutathione-agarose beads, and immunoblotted using anti-His <t>and</t> <t>anti-GST</t> antibodies. (E) Higher energy collisional dissociation (HCD) mass spectrum shows O-fucosylation on the activation loop peptide of MKK4 spanning amino acids 220-242, after in vitro O-fucosylation by SPY.
Anti Gst Antibody, supplied by Abmart Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti gst antibody/product/Abmart Inc
Average 86 stars, based on 1 article reviews
anti gst antibody - by Bioz Stars, 2026-05
86/100 stars
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anti gst antibody  (Beijing Protein Innovation)
86
Beijing Protein Innovation anti gst antibody
(A) MKK5-GFP was affinity purified from 7-day-old WT but not spy-4 seedlings using Aleuria Aurantia Lectin (AAL) immobilized on agarose beads. Coomassie Brilliant Blue staining (CBB) of the membrane shows the loading control. (B) Bio-layer interferometry (BLI) assay showing binding kinetics between SPY and MKK4. 6xHis-SUMO-3TPR-SPY was immobilized to anti-His sensors and dipped into indicated concentrations of GST-MKK4 protein. The dissociation constant (K d ) was calculated by steady-state fitting of GST-MKK4 concentration against the response unit. (C) Co-immunoprecipitation shows in vivo interaction between SPY and MKK5. YFP or SPY-YFP was transiently co-expressed with MKK5-myc in N. benthamiana leaves, immunoprecipitated by anti-GFP antibody, and immunoblotted with anti-GFP or anti-myc antibody. (D) in vitro affinity purification (pull-down) assay shows direct interaction between SPY/SEC and MKK4/5. Recombinant GST, GST-MKK4, and GST-MKK5 were incubated with 6xHis-tagged SPY, SEC, and PCK1, affinity-purified by Glutathione-agarose beads, and immunoblotted using anti-His <t>and</t> <t>anti-GST</t> antibodies. (E) Higher energy collisional dissociation (HCD) mass spectrum shows O-fucosylation on the activation loop peptide of MKK4 spanning amino acids 220-242, after in vitro O-fucosylation by SPY.
Anti Gst Antibody, supplied by Beijing Protein Innovation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti gst antibody/product/Beijing Protein Innovation
Average 86 stars, based on 1 article reviews
anti gst antibody - by Bioz Stars, 2026-05
86/100 stars
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cosmosil gst  (Nacalai)
86
Nacalai cosmosil gst
(A) MKK5-GFP was affinity purified from 7-day-old WT but not spy-4 seedlings using Aleuria Aurantia Lectin (AAL) immobilized on agarose beads. Coomassie Brilliant Blue staining (CBB) of the membrane shows the loading control. (B) Bio-layer interferometry (BLI) assay showing binding kinetics between SPY and MKK4. 6xHis-SUMO-3TPR-SPY was immobilized to anti-His sensors and dipped into indicated concentrations of GST-MKK4 protein. The dissociation constant (K d ) was calculated by steady-state fitting of GST-MKK4 concentration against the response unit. (C) Co-immunoprecipitation shows in vivo interaction between SPY and MKK5. YFP or SPY-YFP was transiently co-expressed with MKK5-myc in N. benthamiana leaves, immunoprecipitated by anti-GFP antibody, and immunoblotted with anti-GFP or anti-myc antibody. (D) in vitro affinity purification (pull-down) assay shows direct interaction between SPY/SEC and MKK4/5. Recombinant GST, GST-MKK4, and GST-MKK5 were incubated with 6xHis-tagged SPY, SEC, and PCK1, affinity-purified by Glutathione-agarose beads, and immunoblotted using anti-His <t>and</t> <t>anti-GST</t> antibodies. (E) Higher energy collisional dissociation (HCD) mass spectrum shows O-fucosylation on the activation loop peptide of MKK4 spanning amino acids 220-242, after in vitro O-fucosylation by SPY.
Cosmosil Gst, supplied by Nacalai, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cosmosil gst/product/Nacalai
Average 86 stars, based on 1 article reviews
cosmosil gst - by Bioz Stars, 2026-05
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gst sepharose 4ff  (Brickell Biotech)
86
Brickell Biotech gst sepharose 4ff
(A) MKK5-GFP was affinity purified from 7-day-old WT but not spy-4 seedlings using Aleuria Aurantia Lectin (AAL) immobilized on agarose beads. Coomassie Brilliant Blue staining (CBB) of the membrane shows the loading control. (B) Bio-layer interferometry (BLI) assay showing binding kinetics between SPY and MKK4. 6xHis-SUMO-3TPR-SPY was immobilized to anti-His sensors and dipped into indicated concentrations of GST-MKK4 protein. The dissociation constant (K d ) was calculated by steady-state fitting of GST-MKK4 concentration against the response unit. (C) Co-immunoprecipitation shows in vivo interaction between SPY and MKK5. YFP or SPY-YFP was transiently co-expressed with MKK5-myc in N. benthamiana leaves, immunoprecipitated by anti-GFP antibody, and immunoblotted with anti-GFP or anti-myc antibody. (D) in vitro affinity purification (pull-down) assay shows direct interaction between SPY/SEC and MKK4/5. Recombinant GST, GST-MKK4, and GST-MKK5 were incubated with 6xHis-tagged SPY, SEC, and PCK1, affinity-purified by Glutathione-agarose beads, and immunoblotted using anti-His <t>and</t> <t>anti-GST</t> antibodies. (E) Higher energy collisional dissociation (HCD) mass spectrum shows O-fucosylation on the activation loop peptide of MKK4 spanning amino acids 220-242, after in vitro O-fucosylation by SPY.
Gst Sepharose 4ff, supplied by Brickell Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gst sepharose 4ff/product/Brickell Biotech
Average 86 stars, based on 1 article reviews
gst sepharose 4ff - by Bioz Stars, 2026-05
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(A) MKK5-GFP was affinity purified from 7-day-old WT but not spy-4 seedlings using Aleuria Aurantia Lectin (AAL) immobilized on agarose beads. Coomassie Brilliant Blue staining (CBB) of the membrane shows the loading control. (B) Bio-layer interferometry (BLI) assay showing binding kinetics between SPY and MKK4. 6xHis-SUMO-3TPR-SPY was immobilized to anti-His sensors and dipped into indicated concentrations of GST-MKK4 protein. The dissociation constant (K d ) was calculated by steady-state fitting of GST-MKK4 concentration against the response unit. (C) Co-immunoprecipitation shows in vivo interaction between SPY and MKK5. YFP or SPY-YFP was transiently co-expressed with MKK5-myc in N. benthamiana leaves, immunoprecipitated by anti-GFP antibody, and immunoblotted with anti-GFP or anti-myc antibody. (D) in vitro affinity purification (pull-down) assay shows direct interaction between SPY/SEC and MKK4/5. Recombinant GST, GST-MKK4, and GST-MKK5 were incubated with 6xHis-tagged SPY, SEC, and PCK1, affinity-purified by Glutathione-agarose beads, and immunoblotted using anti-His and anti-GST antibodies. (E) Higher energy collisional dissociation (HCD) mass spectrum shows O-fucosylation on the activation loop peptide of MKK4 spanning amino acids 220-242, after in vitro O-fucosylation by SPY.

Journal: bioRxiv

Article Title: Linking sugar sensing to immunity in plants via O-glycosylation

doi: 10.64898/2026.05.04.722566

Figure Lengend Snippet: (A) MKK5-GFP was affinity purified from 7-day-old WT but not spy-4 seedlings using Aleuria Aurantia Lectin (AAL) immobilized on agarose beads. Coomassie Brilliant Blue staining (CBB) of the membrane shows the loading control. (B) Bio-layer interferometry (BLI) assay showing binding kinetics between SPY and MKK4. 6xHis-SUMO-3TPR-SPY was immobilized to anti-His sensors and dipped into indicated concentrations of GST-MKK4 protein. The dissociation constant (K d ) was calculated by steady-state fitting of GST-MKK4 concentration against the response unit. (C) Co-immunoprecipitation shows in vivo interaction between SPY and MKK5. YFP or SPY-YFP was transiently co-expressed with MKK5-myc in N. benthamiana leaves, immunoprecipitated by anti-GFP antibody, and immunoblotted with anti-GFP or anti-myc antibody. (D) in vitro affinity purification (pull-down) assay shows direct interaction between SPY/SEC and MKK4/5. Recombinant GST, GST-MKK4, and GST-MKK5 were incubated with 6xHis-tagged SPY, SEC, and PCK1, affinity-purified by Glutathione-agarose beads, and immunoblotted using anti-His and anti-GST antibodies. (E) Higher energy collisional dissociation (HCD) mass spectrum shows O-fucosylation on the activation loop peptide of MKK4 spanning amino acids 220-242, after in vitro O-fucosylation by SPY.

Article Snippet: In brief, bait proteins (final concentration 20 μg/mL) were immobilized onto anti-His (Gator bio #160009) or anti-GST biosensors (Gator bio, #160042) using a Gator-plus instrument (Gator Bio).

Techniques: Affinity Purification, Staining, Membrane, Control, Binding Assay, Concentration Assay, Immunoprecipitation, In Vivo, In Vitro, Pull Down Assay, Recombinant, Incubation, Activation Assay

(A) Bio-layer interferometry (BLI) showing binding kinetics between SPY and MKK5 . 6xHis-SUMO-3TPR-SPY was immobilized to anti-His sensors and dipped into indicated concentrations of GST-MKK5 proteins. The dissociation constant (K d ) was calculated by steady-state fitting of GST-MKK5 concentration against the response unit. (B) BLI assay showing interaction between O-fucosylated GST-MKK5 protein with AAL. GST-MKK5 was incubated with SPY in vitro, with or without GDP-fucose (GF) and SOFTI, loaded onto anti-GST biosensors, and analyzed for binding to AAL.

Journal: bioRxiv

Article Title: Linking sugar sensing to immunity in plants via O-glycosylation

doi: 10.64898/2026.05.04.722566

Figure Lengend Snippet: (A) Bio-layer interferometry (BLI) showing binding kinetics between SPY and MKK5 . 6xHis-SUMO-3TPR-SPY was immobilized to anti-His sensors and dipped into indicated concentrations of GST-MKK5 proteins. The dissociation constant (K d ) was calculated by steady-state fitting of GST-MKK5 concentration against the response unit. (B) BLI assay showing interaction between O-fucosylated GST-MKK5 protein with AAL. GST-MKK5 was incubated with SPY in vitro, with or without GDP-fucose (GF) and SOFTI, loaded onto anti-GST biosensors, and analyzed for binding to AAL.

Article Snippet: In brief, bait proteins (final concentration 20 μg/mL) were immobilized onto anti-His (Gator bio #160009) or anti-GST biosensors (Gator bio, #160042) using a Gator-plus instrument (Gator Bio).

Techniques: Binding Assay, Concentration Assay, Incubation, In Vitro