Journal: Nature

Article Title: Sustained HIV-1 remission after heterozygous CCR5Δ32 stem cell transplantation

doi: 10.1038/s41586-025-09893-0

Figure Lengend Snippet: a , Antibody response dynamics measured as plasma reactivity against HIV-1 Env proteins (gp120, pink; gp140, green) and pseudovirus-neutralizing activity (autologous pseudovirus, orange; Ba-L clade B pseudovirus, blue). pNT, pseudovirus neutralization titre. b , Virus-specific CD8 T cell responses (measured as production of interferon-γ (IFNγ), tumour necrosis factor (TNF) or interleukin-2 (IL-2)) against CMV (pink), EBV (green), HHV-6 (purple) and HIV (light blue) peptide pools at 60, 72 and 81 months after ART interruption. Staphylococcus enterotoxin B (SEB, black) represents the positive control.

Article Snippet: Wells were either coated overnight at 4 °C with 10 μg ml –1 HIV-1 Ba-L_01 gp120 protein (Sino Biological) or left uncoated (PBS only, as control).

Techniques: Clinical Proteomics, Activity Assay, Neutralization, Virus, Positive Control

Journal: bioRxiv

Article Title: M-Sec promotes the production of infectious HIV-1 virus through the exocyst complex

doi: 10.1101/2025.11.13.688207

Figure Lengend Snippet: ( A ) The control or stable M-Sec knockdown (ΔM-Sec) U87 cells were left uninfected or infected with HIV-1, cultured for 2 days, and analyzed for their expression level of Env (gp160 and gp120 in the top blot, and gp41 in the middle blot) by western blotting. β-actin blot is the loading control. ( B ) The control or ΔM-Sec U87 cells were left uninfected or infected with HIV-1, and cultured for 2 days. The virus-like particles in the supernatants were collected by centrifugation, and analyzed for their amount of gp120 (top), gp41 (middle), and p24 Gag (bottom) by western blotting. ( C ) The virus-like particles were analyzed as in B . The density of gp120- or gp41 band was normalized to that of p24 Gag band, and represented as a percentage relative to that of the control cells (n=3). * p < 0.05. ( D , E ) The control or ΔM-Sec U87 cells were infected with HIV-1, and cultured for 2 days. In D , the supernatants were collected, and analyzed for the activity of reverse transcriptase (RT) by qPCR (n=4). In E , the supernatants were collected, and analyzed for viral infectivity using TZM-bl cells as the target cells. Two different inputs were tested (2 and 4 nU/mL of RT activity). The infectivity is represented as a percentage relative to that of the control cells with the input of 2 nU/mL (n=3).

Article Snippet: The antibodies used were as follows: anti-M-Sec (F-6; Santa Cruz Biotechnology), anti-p24 Gag (#65-004; BioAcademia), anti-gp120 Env (KD247; provided by S. Matsushita, Kumamoto University, Japan), anti-gp41 Env (2F5; obtained through BEI Resources, NIAID, NIH), anti-EXOC2 (#12751-1-AP; Proteintech), anti-EXOC3 (#14703-1-AP; Proteintech), and anti-β-actin (EPR16769; Abcam).

Techniques: Control, Knockdown, Infection, Cell Culture, Expressing, Western Blot, Virus, Centrifugation, Activity Assay, Reverse Transcription, Quantitative RT-PCR

Journal: bioRxiv

Article Title: M-Sec promotes the production of infectious HIV-1 virus through the exocyst complex

doi: 10.1101/2025.11.13.688207

Figure Lengend Snippet: ( A ) The parental or stable M-Sec-expressing 293 cells were transfected with the empty plasmid or HIV-1 molecular clone, cultured for 2 days, and analyzed for their expression level of gp160 by western blotting. β-actin blot is the loading control. ( B ) The parental or M-Sec-expressing 293 cells were transfected with the empty plasmid or HIV-1 molecular clone, and cultured for 2 days. The virus-like particles in the supernatants were collected by centrifugation, and analyzed for their amount of gp120 (top), gp41 (middle), and p24 Gag (bottom) by western blotting. ( C ) The cells were analyzed as in B . The density of gp120- or gp41 band was normalized to that of p24 Gag band, and represented as a relative to that of the parental cells (n=3). * p < 0.05. ( D , E ) The parental or M-Sec-expressing 293 cells were transfected with the HIV-1 molecular clone, and cultured for 2 days. In D , the supernatants were collected, and analyzed for the activity of reverse transcriptase (RT) by qPCR (n=5). In E , the supernatants were collected, and analyzed for viral infectivity using TZM-bl cells as the target cells. Two different inputs were tested (2 and 4 nU/mL of RT activity). The infectivity is represented as a percentage relative to that of the parental cells with the input of 2 nU/mL (n=3).

Article Snippet: The antibodies used were as follows: anti-M-Sec (F-6; Santa Cruz Biotechnology), anti-p24 Gag (#65-004; BioAcademia), anti-gp120 Env (KD247; provided by S. Matsushita, Kumamoto University, Japan), anti-gp41 Env (2F5; obtained through BEI Resources, NIAID, NIH), anti-EXOC2 (#12751-1-AP; Proteintech), anti-EXOC3 (#14703-1-AP; Proteintech), and anti-β-actin (EPR16769; Abcam).

Techniques: Expressing, Transfection, Plasmid Preparation, Cell Culture, Western Blot, Control, Virus, Centrifugation, Activity Assay, Reverse Transcription, Quantitative RT-PCR, Infection