Journal: The botulinum journal

Article Title: Exploring genomic diversity in Clostridium botulinum using DNA microarrays

doi: 10.1504/tbj.2012.050195

Figure Lengend Snippet: Scheme used to select probes for the Group I subtyping microarray Notes: A total of 225 probes were selected. 146 probes corresponding to strain variable regions of the ATCC 3502 genome sequence were selected based on CGH analysis using Group I types A, B, and F test strains. In silico analysis was used to select the remaining probes representing genes not present in ATCC 3502, specific for either OkraB or LanglelandF strains, or other specific genes of interest.

Article Snippet: High density CGH microarrays Our laboratory developed a comparative genomic hybridisation (CGH) microarray featuring a total of 384,771 overlapping probes (~50–70 bp) representing the ATCC 3502 type A genome sequence in order to evaluate the level of genomic diversity among C. botulinum strains ( Raphael et al., 2008 ).

Techniques: Microarray, Sequencing, In Silico

Journal: The botulinum journal

Article Title: Exploring genomic diversity in Clostridium botulinum using DNA microarrays

doi: 10.1504/tbj.2012.050195

Figure Lengend Snippet: Matrix of correlation coefficients of the hybridisation results for bont/F5 encoding strains using the Group I subtyping microarray (see online version for colours)

Article Snippet: High density CGH microarrays Our laboratory developed a comparative genomic hybridisation (CGH) microarray featuring a total of 384,771 overlapping probes (~50–70 bp) representing the ATCC 3502 type A genome sequence in order to evaluate the level of genomic diversity among C. botulinum strains ( Raphael et al., 2008 ).

Techniques: Hybridization, Microarray

Journal: The botulinum journal

Article Title: Exploring genomic diversity in Clostridium botulinum using DNA microarrays

doi: 10.1504/tbj.2012.050195

Figure Lengend Snippet: Hybridisation of the Group II subtyping microarray by type E (n = 15), type B (n = 4) and type F (n = 3) C. botulinum strains Notes: Shown is the average % of probes hybridised (i.e., where log10 of the ratio of probe fluorescent compared to background ≥ 1.0) for each toxin serotype indicated. Error bars depict standard deviations.

Article Snippet: High density CGH microarrays Our laboratory developed a comparative genomic hybridisation (CGH) microarray featuring a total of 384,771 overlapping probes (~50–70 bp) representing the ATCC 3502 type A genome sequence in order to evaluate the level of genomic diversity among C. botulinum strains ( Raphael et al., 2008 ).

Techniques: Hybridization, Microarray

Journal: BMJ Case Reports

Article Title: ARHGEF9 disruption in a female patient is associated with X linked mental retardation and sensory hyperarousal

doi: 10.1136/bcr.06.2009.1999

Figure Lengend Snippet: Bacterial artificial chromosome (BAC) position on the X chromosome with fluorescent in situ hybridisation (FISH) results

Article Snippet: The NimbleGen Systems ultra high resolution comparative genomic hybridisation (CGH) microarray platform was used for the detection of copy number polymorphisms.

Techniques: In Situ, Hybridization

Journal: BMJ Case Reports

Article Title: ARHGEF9 disruption in a female patient is associated with X linked mental retardation and sensory hyperarousal

doi: 10.1136/bcr.06.2009.1999

Figure Lengend Snippet: Fluorescent in situ hybridisation (FISH) mapping of the centromeric and telomeric breakpoints with bacterial artificial chromosomes (BACs). (A) FISH with BAC RP11-570J18 shows equal hybridisation at two loci on the inverted X chromosome (arrow) relative to one locus on the unaffected X (arrow head). (B) FISH with RP11-73M8 reveals unequal hybridisation at two loci confirming and narrowing the breakpoint interval (arrow); unaffected X (arrow head). (C) Bracketed region on genome schematic (Xq11.1) outlines estimated breakpoint region as defined by these FISH results (BACs listed in table 1). The centromeric breakpoint lies between ARHGEF9 exons 1–3. (D) Bracketed region on genome schematic (Xq27.2-27.3) depicts estimated telomeric breakpoint region. (FISH data not shown).

Article Snippet: The NimbleGen Systems ultra high resolution comparative genomic hybridisation (CGH) microarray platform was used for the detection of copy number polymorphisms.

Techniques: In Situ, Hybridization