Journal: Parasitology Research

Article Title: Tick-borne pathogens in meat sheep from Brazil: first report of Theileria ovis in sheep in latin America

doi: 10.1007/s00436-025-08587-w

Figure Lengend Snippet: Phylogenetic tree illustrating the evolutionary relationships within the Anaplasma genus, inferred from 23 S rRNA gene sequences (439 nucleotides) using the maximum likelihood method. Bootstrap values greater than 70% are displayed above the branches, indicating statistical confidence in the topology. The scale bar represents an evolutionary distance of 0.05 substitutions per site. Each branch label includes the corresponding GenBank accession number. The kimura 2-parameter model with gamma-distributed rate variation (K2 + G) was determined as the best-fit model. The ≥ 70% threshold is used as a conventional rule-of-thumb for moderate-to-strong support (Hillis and Bull )

Article Snippet: The sequences generated in this study have been submitted to National Center for Biotechnology Information Data base (NCBI) GenBank under the following accession numbers: PV352294 (*Theileria ovis* isolate CL1, 18 S rRNA), PV351966 (*Candidatus* Anaplasma boleense isolate CL14, 16 S rRNA), and PV352147 (*Candidatus* Anaplasma boleense isolate CL14, 23 S rRNA).

Techniques:

Journal: Parasitology Research

Article Title: Tick-borne pathogens in meat sheep from Brazil: first report of Theileria ovis in sheep in latin America

doi: 10.1007/s00436-025-08587-w

Figure Lengend Snippet: Phylogenetic tree depicting the evolutionary relationships within the Anaplasma genus, constructed based on the 16 S rRNA gene sequences (778 nucleotides) using the maximum likelihood method. Bootstrap values represented above the branches are shown for nodes with greater than 70% support. The evolutionary distances, represented by the scale bar, reflect a divergence of 0.04 substitutions per site. GenBank accession numbers are indicated for each sequence. The kimura 2-parameter model with gamma-distributed rate variation (K2 + G) was identified as the optimal evolutionary model. The ≥ 70% threshold is used as a conventional rule-of-thumb for moderate-to-strong support (Hillis and Bull )

Article Snippet: The sequences generated in this study have been submitted to National Center for Biotechnology Information Data base (NCBI) GenBank under the following accession numbers: PV352294 (*Theileria ovis* isolate CL1, 18 S rRNA), PV351966 (*Candidatus* Anaplasma boleense isolate CL14, 16 S rRNA), and PV352147 (*Candidatus* Anaplasma boleense isolate CL14, 23 S rRNA).

Techniques: Construct, Sequencing

Journal: Parasitology Research

Article Title: Tick-borne pathogens in meat sheep from Brazil: first report of Theileria ovis in sheep in latin America

doi: 10.1007/s00436-025-08587-w

Figure Lengend Snippet: Phylogenetic tree illustrating the evolutionary relationships within Theileria ovis , inferred from 18 S rRNA gene sequences (750 nucleotides) using the maximum likelihood method. Bootstrap values greater than 70% are displayed above the branches, indicating statistical confidence in the topology. The scale bar represents an evolutionary distance of 0.02 substitutions per site. Each branch label includes the corresponding GenBank accession number. The tamura–nei model with gamma-distributed rate variation, together with assuming a certain fraction of sites to be evolutionarily invariable (T93 + G + I), was determined as the best-fit model. The ≥ 70% threshold is used as a conventional rule-of-thumb for moderate-to-strong support (Hillis and Bull )

Article Snippet: The sequences generated in this study have been submitted to National Center for Biotechnology Information Data base (NCBI) GenBank under the following accession numbers: PV352294 (*Theileria ovis* isolate CL1, 18 S rRNA), PV351966 (*Candidatus* Anaplasma boleense isolate CL14, 16 S rRNA), and PV352147 (*Candidatus* Anaplasma boleense isolate CL14, 23 S rRNA).

Techniques:

Journal: STAR Protocols

Article Title: Cloning BRD4 long isoform into overexpression vectors for stable overexpression of BRD4-L in mammalian cells

doi: 10.1016/j.xpro.2022.101785

Figure Lengend Snippet:

Article Snippet: Download the appropriate sequences. a. Download the Reference Sequence for Homo sapiens bromodomain containing 4 (BRD4), transcript variant long, mRNA (GenBank: NM_058243.3) from the NCBI’s website, using the GenBank data base ( BRD4-L RefSeq ). b. Download the sequence for LentiV_Blast (Addgene, cat# 111887) from Addgene’s website ( LentiV_Blast ).

Techniques: Virus, Recombinant, Cloning, Gel Extraction, Plasmid Preparation, Sequencing, Over Expression, Expressing, Software, Imaging