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In vivo bone regeneration using a <t>GelMA-LDH</t> composite hydrogel. ( A ) FE-SEM images revealing the highly porous, interconnected microstructure of pure GelMA and GelMA-LDH hydrogels. ( B ) Representative 3D reconstructed micro-CT images of calvarial defects 12 weeks post-implantation in GelMA and GelMA-LDH groups. ( C and D ) Quantitative morphometric analysis of bone volume/tissue volume (BV/TV) and bone mineral density (BMD) from micro-CT data. ( E ) Histological evaluation by H&E and Masson’s Trichrome staining. ( F and G ) Immunofluorescence staining and quantitative analysis of RUNX2 and METTL3 in bone tissue sections from the defect areas. Data are presented as mean ± SD. ***p < 0.001, **p < 0.01.
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Sarstedt gelatin coated dishes
In vivo bone regeneration using a <t>GelMA-LDH</t> composite hydrogel. ( A ) FE-SEM images revealing the highly porous, interconnected microstructure of pure GelMA and GelMA-LDH hydrogels. ( B ) Representative 3D reconstructed micro-CT images of calvarial defects 12 weeks post-implantation in GelMA and GelMA-LDH groups. ( C and D ) Quantitative morphometric analysis of bone volume/tissue volume (BV/TV) and bone mineral density (BMD) from micro-CT data. ( E ) Histological evaluation by H&E and Masson’s Trichrome staining. ( F and G ) Immunofluorescence staining and quantitative analysis of RUNX2 and METTL3 in bone tissue sections from the defect areas. Data are presented as mean ± SD. ***p < 0.001, **p < 0.01.
Gelatin Coated Dishes, supplied by Sarstedt, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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In vivo bone regeneration using a <t>GelMA-LDH</t> composite hydrogel. ( A ) FE-SEM images revealing the highly porous, interconnected microstructure of pure GelMA and GelMA-LDH hydrogels. ( B ) Representative 3D reconstructed micro-CT images of calvarial defects 12 weeks post-implantation in GelMA and GelMA-LDH groups. ( C and D ) Quantitative morphometric analysis of bone volume/tissue volume (BV/TV) and bone mineral density (BMD) from micro-CT data. ( E ) Histological evaluation by H&E and Masson’s Trichrome staining. ( F and G ) Immunofluorescence staining and quantitative analysis of RUNX2 and METTL3 in bone tissue sections from the defect areas. Data are presented as mean ± SD. ***p < 0.001, **p < 0.01.
Gelatin Glycerin, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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In vivo bone regeneration using a <t>GelMA-LDH</t> composite hydrogel. ( A ) FE-SEM images revealing the highly porous, interconnected microstructure of pure GelMA and GelMA-LDH hydrogels. ( B ) Representative 3D reconstructed micro-CT images of calvarial defects 12 weeks post-implantation in GelMA and GelMA-LDH groups. ( C and D ) Quantitative morphometric analysis of bone volume/tissue volume (BV/TV) and bone mineral density (BMD) from micro-CT data. ( E ) Histological evaluation by H&E and Masson’s Trichrome staining. ( F and G ) Immunofluorescence staining and quantitative analysis of RUNX2 and METTL3 in bone tissue sections from the defect areas. Data are presented as mean ± SD. ***p < 0.001, **p < 0.01.
W V Gelatin Macklin G6317, supplied by Macklin Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Shanghai Macklin Biochemical gelatin type b
In vivo bone regeneration using a <t>GelMA-LDH</t> composite hydrogel. ( A ) FE-SEM images revealing the highly porous, interconnected microstructure of pure GelMA and GelMA-LDH hydrogels. ( B ) Representative 3D reconstructed micro-CT images of calvarial defects 12 weeks post-implantation in GelMA and GelMA-LDH groups. ( C and D ) Quantitative morphometric analysis of bone volume/tissue volume (BV/TV) and bone mineral density (BMD) from micro-CT data. ( E ) Histological evaluation by H&E and Masson’s Trichrome staining. ( F and G ) Immunofluorescence staining and quantitative analysis of RUNX2 and METTL3 in bone tissue sections from the defect areas. Data are presented as mean ± SD. ***p < 0.001, **p < 0.01.
Gelatin Type B, supplied by Shanghai Macklin Biochemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Pfizer Inc gelatin sponge
In vivo bone regeneration using a <t>GelMA-LDH</t> composite hydrogel. ( A ) FE-SEM images revealing the highly porous, interconnected microstructure of pure GelMA and GelMA-LDH hydrogels. ( B ) Representative 3D reconstructed micro-CT images of calvarial defects 12 weeks post-implantation in GelMA and GelMA-LDH groups. ( C and D ) Quantitative morphometric analysis of bone volume/tissue volume (BV/TV) and bone mineral density (BMD) from micro-CT data. ( E ) Histological evaluation by H&E and Masson’s Trichrome staining. ( F and G ) Immunofluorescence staining and quantitative analysis of RUNX2 and METTL3 in bone tissue sections from the defect areas. Data are presented as mean ± SD. ***p < 0.001, **p < 0.01.
Gelatin Sponge, supplied by Pfizer Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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In vivo bone regeneration using a GelMA-LDH composite hydrogel. ( A ) FE-SEM images revealing the highly porous, interconnected microstructure of pure GelMA and GelMA-LDH hydrogels. ( B ) Representative 3D reconstructed micro-CT images of calvarial defects 12 weeks post-implantation in GelMA and GelMA-LDH groups. ( C and D ) Quantitative morphometric analysis of bone volume/tissue volume (BV/TV) and bone mineral density (BMD) from micro-CT data. ( E ) Histological evaluation by H&E and Masson’s Trichrome staining. ( F and G ) Immunofluorescence staining and quantitative analysis of RUNX2 and METTL3 in bone tissue sections from the defect areas. Data are presented as mean ± SD. ***p < 0.001, **p < 0.01.

Journal: International Journal of Nanomedicine

Article Title: Size-Dependent Layered Double Hydroxide Nanoparticles Promote Osteogenesis and Bone Regeneration via METTL3-Dependent N6-Methyladenosine Modification of Runx2 mRNA

doi: 10.2147/IJN.S611081

Figure Lengend Snippet: In vivo bone regeneration using a GelMA-LDH composite hydrogel. ( A ) FE-SEM images revealing the highly porous, interconnected microstructure of pure GelMA and GelMA-LDH hydrogels. ( B ) Representative 3D reconstructed micro-CT images of calvarial defects 12 weeks post-implantation in GelMA and GelMA-LDH groups. ( C and D ) Quantitative morphometric analysis of bone volume/tissue volume (BV/TV) and bone mineral density (BMD) from micro-CT data. ( E ) Histological evaluation by H&E and Masson’s Trichrome staining. ( F and G ) Immunofluorescence staining and quantitative analysis of RUNX2 and METTL3 in bone tissue sections from the defect areas. Data are presented as mean ± SD. ***p < 0.001, **p < 0.01.

Article Snippet: Gelatin methacryloyl (GelMA; Advanced BioMatrix, USA) precursor solution (5% w/v) was homogenized with 200 μL of LDH suspension (2 mg/mL) and photo-crosslinked using UV light for 1 min to fabricate GelMA-LDH nanocomposite hydrogels.

Techniques: In Vivo, Micro-CT, Staining, Immunofluorescence