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PIK3CB and SP1 were overexpressed in GC samples. (A) The top 10 genes co-expressed with PIK3CB in TCGA-STAD datasets were shown. PCC: Pearson correlation coefficient. (B) The expression relevance between PIK3CB and SP1 was predicted by GEPIA online tools. (C) PIK3CB and SP1 were upregulated in GC tissue samples form TCGA database using GEPIA. (D) Kaplan-Meier curves for patients with different expression levels of PIK3CB or SP1 via Kaplan-Meier plotter database. (E) qRT-PCR analysis of PIK3CB and SP1 expression in 40 paired GC tissue and adjacent normal tissues. C/N score ≥1.5 was viewed as upregulated. (F) The assessment of correlation between PIK3CB and SP1 expression in 40 paired GC tissues using linear regression. (G) PIK3CB and SP1 expression level were evaluated by immunohistochemistry staining on a GC tissue <t>microarray.</t> Representative staining images and expression correlation of PI3KCB and SP1 in tumor tissue were shown. Scale bar: 50 μm. *P < 0.01.
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PIK3CB and SP1 were overexpressed in GC samples. (A) The top 10 genes co-expressed with PIK3CB in TCGA-STAD datasets were shown. PCC: Pearson correlation coefficient. (B) The expression relevance between PIK3CB and SP1 was predicted by GEPIA online tools. (C) PIK3CB and SP1 were upregulated in GC tissue samples form TCGA database using GEPIA. (D) Kaplan-Meier curves for patients with different expression levels of PIK3CB or SP1 via Kaplan-Meier plotter database. (E) qRT-PCR analysis of PIK3CB and SP1 expression in 40 paired GC tissue and adjacent normal tissues. C/N score ≥1.5 was viewed as upregulated. (F) The assessment of correlation between PIK3CB and SP1 expression in 40 paired GC tissues using linear regression. (G) PIK3CB and SP1 expression level were evaluated by immunohistochemistry staining on a GC tissue <t>microarray.</t> Representative staining images and expression correlation of PI3KCB and SP1 in tumor tissue were shown. Scale bar: 50 μm. *P < 0.01.
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PIK3CB and SP1 were overexpressed in GC samples. (A) The top 10 genes co-expressed with PIK3CB in TCGA-STAD datasets were shown. PCC: Pearson correlation coefficient. (B) The expression relevance between PIK3CB and SP1 was predicted by GEPIA online tools. (C) PIK3CB and SP1 were upregulated in GC tissue samples form TCGA database using GEPIA. (D) Kaplan-Meier curves for patients with different expression levels of PIK3CB or SP1 via Kaplan-Meier plotter database. (E) qRT-PCR analysis of PIK3CB and SP1 expression in 40 paired GC tissue and adjacent normal tissues. C/N score ≥1.5 was viewed as upregulated. (F) The assessment of correlation between PIK3CB and SP1 expression in 40 paired GC tissues using linear regression. (G) PIK3CB and SP1 expression level were evaluated by immunohistochemistry staining on a GC tissue <t>microarray.</t> Representative staining images and expression correlation of PI3KCB and SP1 in tumor tissue were shown. Scale bar: 50 μm. *P < 0.01.
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PIK3CB and SP1 were overexpressed in GC samples. (A) The top 10 genes co-expressed with PIK3CB in TCGA-STAD datasets were shown. PCC: Pearson correlation coefficient. (B) The expression relevance between PIK3CB and SP1 was predicted by GEPIA online tools. (C) PIK3CB and SP1 were upregulated in GC tissue samples form TCGA database using GEPIA. (D) Kaplan-Meier curves for patients with different expression levels of PIK3CB or SP1 via Kaplan-Meier plotter database. (E) qRT-PCR analysis of PIK3CB and SP1 expression in 40 paired GC tissue and adjacent normal tissues. C/N score ≥1.5 was viewed as upregulated. (F) The assessment of correlation between PIK3CB and SP1 expression in 40 paired GC tissues using linear regression. (G) PIK3CB and SP1 expression level were evaluated by immunohistochemistry staining on a GC tissue microarray. Representative staining images and expression correlation of PI3KCB and SP1 in tumor tissue were shown. Scale bar: 50 μm. *P < 0.01.

Journal: Journal of Cancer

Article Title: SP1 Mediated PIK3CB Upregulation Promotes Gastric Carcinogenesis

doi: 10.7150/jca.83812

Figure Lengend Snippet: PIK3CB and SP1 were overexpressed in GC samples. (A) The top 10 genes co-expressed with PIK3CB in TCGA-STAD datasets were shown. PCC: Pearson correlation coefficient. (B) The expression relevance between PIK3CB and SP1 was predicted by GEPIA online tools. (C) PIK3CB and SP1 were upregulated in GC tissue samples form TCGA database using GEPIA. (D) Kaplan-Meier curves for patients with different expression levels of PIK3CB or SP1 via Kaplan-Meier plotter database. (E) qRT-PCR analysis of PIK3CB and SP1 expression in 40 paired GC tissue and adjacent normal tissues. C/N score ≥1.5 was viewed as upregulated. (F) The assessment of correlation between PIK3CB and SP1 expression in 40 paired GC tissues using linear regression. (G) PIK3CB and SP1 expression level were evaluated by immunohistochemistry staining on a GC tissue microarray. Representative staining images and expression correlation of PI3KCB and SP1 in tumor tissue were shown. Scale bar: 50 μm. *P < 0.01.

Article Snippet: The human GC tissue microarray containing 48 gastric cancer tissues and adjacent normal tissues were purchased from Zhuoli Biotech (Shanghai, China).

Techniques: Expressing, Quantitative RT-PCR, Immunohistochemistry, Staining, Microarray