Journal: Biomolecules

Article Title: Pro-Calcifying Role of Enzymatically Modified LDL (eLDL) in Aortic Valve Sclerosis via Induction of IL-6 and IL-33

doi: 10.3390/biom13071091

Figure Lengend Snippet: The primary antibodies used for immunohistochemistry and Western blot analysis.

Article Snippet: Hsp27 , HSP27 (G31) mAb , IHC-P , 1:200 , Mouse , Cell Signaling Technology.

Techniques: Immunohistochemistry, Western Blot

Journal: Biomolecules

Article Title: Pro-Calcifying Role of Enzymatically Modified LDL (eLDL) in Aortic Valve Sclerosis via Induction of IL-6 and IL-33

doi: 10.3390/biom13071091

Figure Lengend Snippet: p38 MAPK and eLDL in human aortic valves. ( A ) Detection of rapidly phosphorylated p38 MAPK upon treatment of VICs/myofibroblasts with eLDL. Cells were exposed to 10 or 20 µg/mL eLDL for 30 min. Cells without treatment served as control. Phosphorylated p38 MAPK and total p38 MAPK in whole-cell lysates were sequentially detected by Western blot analysis on the same membrane. Blots were re-probed with β-actin antibody to confirm equal loading. ( B ) WB quantification of the phosphorylation of p38 MAPK. ( C ) Expression of p38 MAPK isoforms in VICs/myofibroblasts. Relative mRNA levels for p38 subtypes quantified in VICs/myofibroblasts from 10 different patients using real-time PCR. Expression levels are given as RQ of target genes (p38α/MAPK14, p38β/MAPK11, p38γ/MAPK12, p38δ/MAPK13) normalized to the reference gene GAPDH. ( D ) Representative immunohistochemical staining of aortic valves sections stained with ( a ), anti-p38; ( b ), anti-phospho-p38; ( c ), anti-Hsp27; ( d ), anti-phospho-Hsp27. The fibrosa with the aortic side of the valve is on top.

Article Snippet: Hsp27 , HSP27 (G31) mAb , IHC-P , 1:200 , Mouse , Cell Signaling Technology.

Techniques: Control, Western Blot, Membrane, Phospho-proteomics, Expressing, Real-time Polymerase Chain Reaction, Immunohistochemical staining, Staining

Journal: Materials

Article Title: Mechanical Resistance and Tissue Structure of Claw Denticles of Various Sizes in the Mud Crab, Scylla serrata

doi: 10.3390/ma16114114

Figure Lengend Snippet: Optical micrographs of a cross-section of the entire fixed finger of the claw after polishing and the hardness at four characteristic areas were obtained via Vickers tests. The tests were performed more than five times for each site, and the site is 100–200 μm away from the outer and inner surfaces. Here, den(S) and den(M) denote small and medium denticles, and den(L1) and den(L2) denote large denticles .

Article Snippet: The Vickers and nanoindentation tests were conducted using a Shimadzu Micro Vickers Hardness Tester, HMV-G31 (SHIMADZU, Kyoto, Japan), and an ENT-NEXUS (ELIONIX, Tokyo, Japan).

Techniques: