Journal: Biomolecules

Article Title: Notch Signaling Exacerbates Pulmonary Fibrosis by Regulating the Differentiation of CD4 + Tissue-Resident Memory T Cells

doi: 10.3390/biom16020328

Figure Lengend Snippet: Recruitment of circulating lymphocytes is a major source of lung CD4 + T RM cells in BLM-induced PF. ( A ) Schematic diagram of the mouse treatment schedule. FTY720 (1 mg/kg) or PBS was administered intraperitoneally once daily from 3 days before (D-3) to 14 days after (D14) BLM-induced PF. ( B ) Representative flow cytometry plots of CD3 + CD8 − (CD4 + ) and CD3 + CD8 + T cell frequencies among peripheral blood CD45 + cells in both groups, taken 1 day before (D-1) BLM administration. ( C ) Quantitative analysis of CD3 + CD4 + T cell proportion and absolute number in peripheral blood CD45 + cells from both groups at D-1. n = 6 in FTY720 group, and n = 5 in Vehicle group. ( D ) Quantitative analysis of CD3 + CD8 + T cell proportion and absolute number in peripheral blood CD45 + cells from both groups at D-1. n = 6 in FTY720 group, and n = 5 in Vehicle group. ( E ) Representative flow cytometry plots showing the proportions of CD3 + CD4 + and CD3 + CD8 + T cells among peripheral blood CD45 + cells in both groups on D14. ( F ) Quantitative analysis of CD3 + CD4 + T cell proportion and absolute number in peripheral blood CD45 + cells from both groups on D14. n = 6 for each group. ( G ) Quantitative analysis of CD3 + CD8 + T cell proportion and absolute number in peripheral blood CD45 + cells from both groups on D14. n = 6 for each group. ( H ) Representative contour plots of CD3 and CD8 expression in lung CD45 + T cells. (I–L) The absolute numbers of CD4 + T cells ( I ), CD8 + T cells ( J ), CD4 + non-T RM cells ( K ) and CD4 + CD69 + CD103 + T RM cells ( L ) in the lung were measured by flow cytometry. n = 6 for each group. Data were expressed as mean ± SEM. Student’s t test panel ( C , D , F , G , I – L ). *** p < 0.001, **** p < 0.0001.

Article Snippet: To investigate the origin of lung T RM cells in PF, FTY720 (MCE, Monmouth Junction, NJ, USA, Cat#: HY-12005, 1 mg/kg) or vehicle was administered via intraperitoneal injection starting three days before BLM exposure and continued daily thereafter.

Techniques: Flow Cytometry, Expressing

Journal: Biomolecules

Article Title: Notch Signaling Exacerbates Pulmonary Fibrosis by Regulating the Differentiation of CD4 + Tissue-Resident Memory T Cells

doi: 10.3390/biom16020328

Figure Lengend Snippet: Inhibition of the recruitment of circulating lymphocytes alleviated BLM-induced PF. ( A ) Schematic diagram of the mouse treatment schedule. FTY720 (1 mg/kg) or PBS was administered intraperitoneally once daily from 3 days before (D-3) to 14 days after (D14) BLM-induced PF. n = 6 for each group. ( B ) Body weight loss was monitored daily. ( C ) Mouse lung images. ( D ) Mouse lung index. ( E , F ) H&E staining of lung sections ( E ) and Ashcroft scores ( F ) from the two groups of mice. ( G , H ) Masson staining of lung sections ( G ) and collagen volume fraction ( H ) in the two groups. ( I , J ) Immunohistochemistry staining for α-SMA in lung sections ( I ) and quantitative analysis of its integrated optical density (IOD) ( J ) in the two groups. ( K ) Fibrosis marker expressions were detected by qPCR. Data were expressed as mean ± SEM. Student’s t test panel ( B , D , F , H , J , K ). * p < 0.05, ** p < 0.01, ns: not significant.

Article Snippet: To investigate the origin of lung T RM cells in PF, FTY720 (MCE, Monmouth Junction, NJ, USA, Cat#: HY-12005, 1 mg/kg) or vehicle was administered via intraperitoneal injection starting three days before BLM exposure and continued daily thereafter.

Techniques: Inhibition, Staining, Immunohistochemistry, Marker

Journal: bioRxiv

Article Title: Indomethacin exerts both cyclooxygenase inhibition-dependent and independent mechanisms to enhance chemo-immunotherapy in mice

doi: 10.64898/2026.01.07.698231

Figure Lengend Snippet: (A) Indo fails to enhance CTX efficacy in immunodeficient NSG mice. The schema depicts the timeline of experimental procedures. NSG mice bearing established CT26 tumors (60–90 mm²) were randomized into four groups and treated as indicated. Tumor growth curves are shown. (B) Kaplan–Meier survival analysis of the same cohort. Data were pooled from two independent experiments. (C) The beneficial effect of Indo requires endogenous CD8⁺ T cells. The schema depicts the timeline of experimental procedures. BALB/c mice bearing established CT26 tumors (60–90 mm²) were treated with CTX+Indo. A subset of mice additionally received i.p. anti-CD8 Ab before and during treatment to deplete endogenous CD8⁺ T cells. Mice treated with CTX alone were included as controls. Tumor growth curves are shown. Mouse survival is summarized in the Kaplan-Meier plot (D). (E) Blockade of T cell trafficking by FTY720 diminishes the efficacy of CTX+Indo. BALB/c mice with established CT26 tumors (60–90 mm²) were treated with CTX+Indo. These mice also received either FTY720 or solvent by i.p. injection three times weekly for 4 weeks, starting one day prior to CTX administration. Tumor growth curves are shown. (F) Kaplan–Meier survival analysis. Data were pooled from two independent experiments. Data were pooled from two independent experiments. Statistics: (B, D, F) Log-rank (Mantel-Cox) test. **, p < 0.01; ***, p < 0.001; ns, not significant.

Article Snippet: Fingolimod hydrochloride (FTY720) was purchased from MedChemExpress.

Techniques: Solvent, Injection

Journal: bioRxiv

Article Title: Indomethacin exerts both cyclooxygenase inhibition-dependent and independent mechanisms to enhance chemo-immunotherapy in mice

doi: 10.64898/2026.01.07.698231

Figure Lengend Snippet: (A) FTY720 administration reduces T cells in circulation but does not alter T cell frequency in secondary lymphoid organs. CT26-bearing BALB/c mice were treated as described in . A cohort of mice were euthanized after receiving 4 doses of FTY720 or solvent. The frequencies of CD4+ and CD8+ T cells in blood, draining lymph node and spleen were determined by flow cytometry. Representative dot plots are shown. Numbers in plots denote percent of CD4+ and CD8+ T cells. (B) FTY720 administration diminishes the efficacy of CTX+Indo in MC38 tumor model. Following the same experimental procedures depicted in , C57BL/6 mice with established MC38 tumors (60-90mm 2 ) were treated as indicated. Tumor growth curves are shown with mice numbers in each group indicated. Mouse survival is summarized in the Kaplan-Meier plot (C). Statistics: Log-rank (Mantel-Cox) test. *, p < 0.05; ns, not significant.

Article Snippet: Fingolimod hydrochloride (FTY720) was purchased from MedChemExpress.

Techniques: Solvent, Flow Cytometry