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cattle fluorescent resonance engery transfers frets vwf71 substrate  (Versiti Inc)
86
Versiti Inc cattle fluorescent resonance engery transfers frets vwf71 substrate
Generation of A13-null rats by CRISPR/Cas9. (A) Schematic domain structure of rat A13 protein and the potential targeted region by CRISPR/Cas9 (arrowhead). (B) Sanger sequencing demonstrates the results of WT and KO sequence with a 13–base pair (bp) deletion (−14+1 bp; red arrowhead) in the F2 progeny rat. (C) Relative abundance of A13 mRNA (median, IQR) by RT-PCR in the liver tissues of WT (n = 8) and KO (n = 8) rats. (D) Western blotting detects a positive ADAMTS13 (A13) band (arrowhead) in the lysate of HEK293 cells expressing human rA13 and in the liver tissue lysate of WT but not of KO rats. (E) Plasma A13 activity (individual values, median, and IQR) determined by the cleavage of a cattle <t>FRETS-VWF71</t> in WT (n = 10), Het (n = 10), and KO (n = 11) rats. Mann-Whitney U and Kruskal-Willis tests were performed for statistical significance between 2 groups and among 3 different groups, respectively. Here, ∗∗∗ and ∗∗∗∗ indicate P values of <.005 and <.0001, respectively. A13, ADAMTS13; rA13, recombinant ADAMTS13.
Cattle Fluorescent Resonance Engery Transfers Frets Vwf71 Substrate, supplied by Versiti Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fluorescence resonance energy transfer substrate frets vwf73 method  (Peptide Institute)
86
Peptide Institute fluorescence resonance energy transfer substrate frets vwf73 method
Generation of A13-null rats by CRISPR/Cas9. (A) Schematic domain structure of rat A13 protein and the potential targeted region by CRISPR/Cas9 (arrowhead). (B) Sanger sequencing demonstrates the results of WT and KO sequence with a 13–base pair (bp) deletion (−14+1 bp; red arrowhead) in the F2 progeny rat. (C) Relative abundance of A13 mRNA (median, IQR) by RT-PCR in the liver tissues of WT (n = 8) and KO (n = 8) rats. (D) Western blotting detects a positive ADAMTS13 (A13) band (arrowhead) in the lysate of HEK293 cells expressing human rA13 and in the liver tissue lysate of WT but not of KO rats. (E) Plasma A13 activity (individual values, median, and IQR) determined by the cleavage of a cattle <t>FRETS-VWF71</t> in WT (n = 10), Het (n = 10), and KO (n = 11) rats. Mann-Whitney U and Kruskal-Willis tests were performed for statistical significance between 2 groups and among 3 different groups, respectively. Here, ∗∗∗ and ∗∗∗∗ indicate P values of <.005 and <.0001, respectively. A13, ADAMTS13; rA13, recombinant ADAMTS13.
Fluorescence Resonance Energy Transfer Substrate Frets Vwf73 Method, supplied by Peptide Institute, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fluorescence resonance energy transfer substrate frets vwf73 method - by Bioz Stars, 2026-06
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plk1 fret sensor c jun substrate plasmid 32  (Addgene inc)
93
Addgene inc plk1 fret sensor c jun substrate plasmid 32
Generation of A13-null rats by CRISPR/Cas9. (A) Schematic domain structure of rat A13 protein and the potential targeted region by CRISPR/Cas9 (arrowhead). (B) Sanger sequencing demonstrates the results of WT and KO sequence with a 13–base pair (bp) deletion (−14+1 bp; red arrowhead) in the F2 progeny rat. (C) Relative abundance of A13 mRNA (median, IQR) by RT-PCR in the liver tissues of WT (n = 8) and KO (n = 8) rats. (D) Western blotting detects a positive ADAMTS13 (A13) band (arrowhead) in the lysate of HEK293 cells expressing human rA13 and in the liver tissue lysate of WT but not of KO rats. (E) Plasma A13 activity (individual values, median, and IQR) determined by the cleavage of a cattle <t>FRETS-VWF71</t> in WT (n = 10), Het (n = 10), and KO (n = 11) rats. Mann-Whitney U and Kruskal-Willis tests were performed for statistical significance between 2 groups and among 3 different groups, respectively. Here, ∗∗∗ and ∗∗∗∗ indicate P values of <.005 and <.0001, respectively. A13, ADAMTS13; rA13, recombinant ADAMTS13.
Plk1 Fret Sensor C Jun Substrate Plasmid 32, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plk1 fret sensor c jun substrate plasmid 32 - by Bioz Stars, 2026-06
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c jun based plk1 fret sensor  (Addgene inc)
93
Addgene inc c jun based plk1 fret sensor
Generation of A13-null rats by CRISPR/Cas9. (A) Schematic domain structure of rat A13 protein and the potential targeted region by CRISPR/Cas9 (arrowhead). (B) Sanger sequencing demonstrates the results of WT and KO sequence with a 13–base pair (bp) deletion (−14+1 bp; red arrowhead) in the F2 progeny rat. (C) Relative abundance of A13 mRNA (median, IQR) by RT-PCR in the liver tissues of WT (n = 8) and KO (n = 8) rats. (D) Western blotting detects a positive ADAMTS13 (A13) band (arrowhead) in the lysate of HEK293 cells expressing human rA13 and in the liver tissue lysate of WT but not of KO rats. (E) Plasma A13 activity (individual values, median, and IQR) determined by the cleavage of a cattle <t>FRETS-VWF71</t> in WT (n = 10), Het (n = 10), and KO (n = 11) rats. Mann-Whitney U and Kruskal-Willis tests were performed for statistical significance between 2 groups and among 3 different groups, respectively. Here, ∗∗∗ and ∗∗∗∗ indicate P values of <.005 and <.0001, respectively. A13, ADAMTS13; rA13, recombinant ADAMTS13.
C Jun Based Plk1 Fret Sensor, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fret substrate  (Bio-Rad)
99
Bio-Rad fret substrate
Generation of A13-null rats by CRISPR/Cas9. (A) Schematic domain structure of rat A13 protein and the potential targeted region by CRISPR/Cas9 (arrowhead). (B) Sanger sequencing demonstrates the results of WT and KO sequence with a 13–base pair (bp) deletion (−14+1 bp; red arrowhead) in the F2 progeny rat. (C) Relative abundance of A13 mRNA (median, IQR) by RT-PCR in the liver tissues of WT (n = 8) and KO (n = 8) rats. (D) Western blotting detects a positive ADAMTS13 (A13) band (arrowhead) in the lysate of HEK293 cells expressing human rA13 and in the liver tissue lysate of WT but not of KO rats. (E) Plasma A13 activity (individual values, median, and IQR) determined by the cleavage of a cattle <t>FRETS-VWF71</t> in WT (n = 10), Het (n = 10), and KO (n = 11) rats. Mann-Whitney U and Kruskal-Willis tests were performed for statistical significance between 2 groups and among 3 different groups, respectively. Here, ∗∗∗ and ∗∗∗∗ indicate P values of <.005 and <.0001, respectively. A13, ADAMTS13; rA13, recombinant ADAMTS13.
Fret Substrate, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fret substrate - by Bioz Stars, 2026-06
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plk1 fret sensor c jun substrate plasmid  (Addgene inc)
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Addgene inc plk1 fret sensor c jun substrate plasmid
Generation of A13-null rats by CRISPR/Cas9. (A) Schematic domain structure of rat A13 protein and the potential targeted region by CRISPR/Cas9 (arrowhead). (B) Sanger sequencing demonstrates the results of WT and KO sequence with a 13–base pair (bp) deletion (−14+1 bp; red arrowhead) in the F2 progeny rat. (C) Relative abundance of A13 mRNA (median, IQR) by RT-PCR in the liver tissues of WT (n = 8) and KO (n = 8) rats. (D) Western blotting detects a positive ADAMTS13 (A13) band (arrowhead) in the lysate of HEK293 cells expressing human rA13 and in the liver tissue lysate of WT but not of KO rats. (E) Plasma A13 activity (individual values, median, and IQR) determined by the cleavage of a cattle <t>FRETS-VWF71</t> in WT (n = 10), Het (n = 10), and KO (n = 11) rats. Mann-Whitney U and Kruskal-Willis tests were performed for statistical significance between 2 groups and among 3 different groups, respectively. Here, ∗∗∗ and ∗∗∗∗ indicate P values of <.005 and <.0001, respectively. A13, ADAMTS13; rA13, recombinant ADAMTS13.
Plk1 Fret Sensor C Jun Substrate Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plk1 fret sensor c jun substrate plasmid - by Bioz Stars, 2026-06
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fret substrate  (Peptide Institute)
86
Peptide Institute fret substrate
Generation of A13-null rats by CRISPR/Cas9. (A) Schematic domain structure of rat A13 protein and the potential targeted region by CRISPR/Cas9 (arrowhead). (B) Sanger sequencing demonstrates the results of WT and KO sequence with a 13–base pair (bp) deletion (−14+1 bp; red arrowhead) in the F2 progeny rat. (C) Relative abundance of A13 mRNA (median, IQR) by RT-PCR in the liver tissues of WT (n = 8) and KO (n = 8) rats. (D) Western blotting detects a positive ADAMTS13 (A13) band (arrowhead) in the lysate of HEK293 cells expressing human rA13 and in the liver tissue lysate of WT but not of KO rats. (E) Plasma A13 activity (individual values, median, and IQR) determined by the cleavage of a cattle <t>FRETS-VWF71</t> in WT (n = 10), Het (n = 10), and KO (n = 11) rats. Mann-Whitney U and Kruskal-Willis tests were performed for statistical significance between 2 groups and among 3 different groups, respectively. Here, ∗∗∗ and ∗∗∗∗ indicate P values of <.005 and <.0001, respectively. A13, ADAMTS13; rA13, recombinant ADAMTS13.
Fret Substrate, supplied by Peptide Institute, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fret substrate - by Bioz Stars, 2026-06
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fret based protease substrates  (Radboud University)
86
Radboud University fret based protease substrates
Generation of A13-null rats by CRISPR/Cas9. (A) Schematic domain structure of rat A13 protein and the potential targeted region by CRISPR/Cas9 (arrowhead). (B) Sanger sequencing demonstrates the results of WT and KO sequence with a 13–base pair (bp) deletion (−14+1 bp; red arrowhead) in the F2 progeny rat. (C) Relative abundance of A13 mRNA (median, IQR) by RT-PCR in the liver tissues of WT (n = 8) and KO (n = 8) rats. (D) Western blotting detects a positive ADAMTS13 (A13) band (arrowhead) in the lysate of HEK293 cells expressing human rA13 and in the liver tissue lysate of WT but not of KO rats. (E) Plasma A13 activity (individual values, median, and IQR) determined by the cleavage of a cattle <t>FRETS-VWF71</t> in WT (n = 10), Het (n = 10), and KO (n = 11) rats. Mann-Whitney U and Kruskal-Willis tests were performed for statistical significance between 2 groups and among 3 different groups, respectively. Here, ∗∗∗ and ∗∗∗∗ indicate P values of <.005 and <.0001, respectively. A13, ADAMTS13; rA13, recombinant ADAMTS13.
Fret Based Protease Substrates, supplied by Radboud University, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fret based protease substrates - by Bioz Stars, 2026-06
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adamts 13 specifc fluorescent frets vwf73 substrate  (Peptide Institute)
86
Peptide Institute adamts 13 specifc fluorescent frets vwf73 substrate
Generation of A13-null rats by CRISPR/Cas9. (A) Schematic domain structure of rat A13 protein and the potential targeted region by CRISPR/Cas9 (arrowhead). (B) Sanger sequencing demonstrates the results of WT and KO sequence with a 13–base pair (bp) deletion (−14+1 bp; red arrowhead) in the F2 progeny rat. (C) Relative abundance of A13 mRNA (median, IQR) by RT-PCR in the liver tissues of WT (n = 8) and KO (n = 8) rats. (D) Western blotting detects a positive ADAMTS13 (A13) band (arrowhead) in the lysate of HEK293 cells expressing human rA13 and in the liver tissue lysate of WT but not of KO rats. (E) Plasma A13 activity (individual values, median, and IQR) determined by the cleavage of a cattle <t>FRETS-VWF71</t> in WT (n = 10), Het (n = 10), and KO (n = 11) rats. Mann-Whitney U and Kruskal-Willis tests were performed for statistical significance between 2 groups and among 3 different groups, respectively. Here, ∗∗∗ and ∗∗∗∗ indicate P values of <.005 and <.0001, respectively. A13, ADAMTS13; rA13, recombinant ADAMTS13.
Adamts 13 Specifc Fluorescent Frets Vwf73 Substrate, supplied by Peptide Institute, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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adamts 13 specifc fluorescent frets vwf73 substrate - by Bioz Stars, 2026-06
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Image Search Results


Generation of A13-null rats by CRISPR/Cas9. (A) Schematic domain structure of rat A13 protein and the potential targeted region by CRISPR/Cas9 (arrowhead). (B) Sanger sequencing demonstrates the results of WT and KO sequence with a 13–base pair (bp) deletion (−14+1 bp; red arrowhead) in the F2 progeny rat. (C) Relative abundance of A13 mRNA (median, IQR) by RT-PCR in the liver tissues of WT (n = 8) and KO (n = 8) rats. (D) Western blotting detects a positive ADAMTS13 (A13) band (arrowhead) in the lysate of HEK293 cells expressing human rA13 and in the liver tissue lysate of WT but not of KO rats. (E) Plasma A13 activity (individual values, median, and IQR) determined by the cleavage of a cattle FRETS-VWF71 in WT (n = 10), Het (n = 10), and KO (n = 11) rats. Mann-Whitney U and Kruskal-Willis tests were performed for statistical significance between 2 groups and among 3 different groups, respectively. Here, ∗∗∗ and ∗∗∗∗ indicate P values of <.005 and <.0001, respectively. A13, ADAMTS13; rA13, recombinant ADAMTS13.

Journal: Blood Advances

Article Title: Rat models of thrombotic thrombocytopenic purpura reveal crucial role of placental ADAMTS13 in perinatal survival ∗

doi: 10.1182/bloodadvances.2026019661

Figure Lengend Snippet: Generation of A13-null rats by CRISPR/Cas9. (A) Schematic domain structure of rat A13 protein and the potential targeted region by CRISPR/Cas9 (arrowhead). (B) Sanger sequencing demonstrates the results of WT and KO sequence with a 13–base pair (bp) deletion (−14+1 bp; red arrowhead) in the F2 progeny rat. (C) Relative abundance of A13 mRNA (median, IQR) by RT-PCR in the liver tissues of WT (n = 8) and KO (n = 8) rats. (D) Western blotting detects a positive ADAMTS13 (A13) band (arrowhead) in the lysate of HEK293 cells expressing human rA13 and in the liver tissue lysate of WT but not of KO rats. (E) Plasma A13 activity (individual values, median, and IQR) determined by the cleavage of a cattle FRETS-VWF71 in WT (n = 10), Het (n = 10), and KO (n = 11) rats. Mann-Whitney U and Kruskal-Willis tests were performed for statistical significance between 2 groups and among 3 different groups, respectively. Here, ∗∗∗ and ∗∗∗∗ indicate P values of <.005 and <.0001, respectively. A13, ADAMTS13; rA13, recombinant ADAMTS13.

Article Snippet: A cattle fluorescent resonance engery transfers (FRETS)-VWF71 substrate (kindly provided by Josua Muia from Versiti, Milwaukee, WI), as described previously, was used for the ADAMTS13 activity assay.

Techniques: CRISPR, Sequencing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Expressing, Clinical Proteomics, Activity Assay, MANN-WHITNEY, Recombinant