Journal: Microorganisms

Article Title: Resistance and Virulence Surveillance in Escherichia coli Isolated from Commercial Meat Samples: A One Health Approach

doi: 10.3390/microorganisms11112712

Figure Lengend Snippet: Comparative antimicrobial susceptibility profile across beef, chicken, and pork samples. On the left a figure relating to the number of isolates per meat sample and on the right a figure relating to the percentage of resistance per meat sample. FOT—fosfomycin-trometamol; TET—tetracycline; SXT—trimethoprim-sulfamethoxazole; C—chloramphenicol; CN—gentamicin; CIP—ciprofloxacin; AMC—amoxicillin-clavulanic acid; AMP—ampicillin; CFZ—cefazolin; CFO—cefoxitin; CRO—ceftriaxone; CAZ—ceftazidime; ATM—aztreonam.

Article Snippet: Antimicrobial sensitivity was determined using the disk diffusion method [ ], with seven distinct classes of antimicrobials: (1) β-lactams: ampicillin (AMP, 10 μg), amoxicillin-clavulanic acid (AMC, 10/20 μg), cefazolin (CFZ, 30 μg), cefoxitin, (CFO, 30 μg), ceftriaxone (CRO, 30 μg), ceftazidime (CAZ, 30 μg), cefotaxime (CTX, 30 μg), aztreonam (ATM, 30 μg), and imipenem (IPM, 30 μg); (2) quinolones: ciprofloxacin (CIP, 5 μg); (3) sulfonamides: sulfamethoxazole + trimethoprim (SXT, 1.25/23.75 μg); (4) tetracyclines: tetracycline (TET, 30 μg); (5) aminoglycosides: gentamicin (CN, 10 μg); (6) amphenicols: chloramphenicol (C, 30 μg); and (7) fosfomycin: fosfomycin/trometamol (FOT, 200 μg; Oxoid Ltd., Basingstoke, Hants, United Kingdom).

Techniques:

Journal: Pharmaceutics

Article Title: New Imidazolium Alkaloids with Broad Spectrum of Action from the Marine Bacterium Shewanella aquimarina

doi: 10.3390/pharmaceutics15082139

Figure Lengend Snippet: Kirby–Bauer synergy assay against MRSA, in which the effects of the antibiotics are represented by clear halos. In ( A ), the agar does not contain the active fraction, while in ( B ) the fraction F2 is present. The presence of the fraction increased the antibacterial activity of TGC (red), LZD (light blue), MFX (orange), AMP (purple), and TBG (dark blue). Notably, it completely restored the antibacterial activity of the fosfomycin (yellow).

Article Snippet: Antibiotics The following antibiotics loaded on disks were utilized in the Kirby–Bauer assay: moxifloxacin (MFX) 5 µg, fosfomycin (FOT) 200 µg, erythromycin (E) 15 µg, linezolid (LZD) 10 µg, 30 µg, and tigecycline (TGC) 15 µg, purchased from Mast Group Ltd. (Bootle, UK), while ampicillin (AMP) 10 µg, cefepime (FEP) 30 µg, teicoplanin (TEC), and gentamycin (CN) 15 µg were bought from Oxoid Ltd. (Thermo Fisher Scientific, Waltham, MA, USA).

Techniques: Activity Assay

Journal: Pharmaceutics

Article Title: New Imidazolium Alkaloids with Broad Spectrum of Action from the Marine Bacterium Shewanella aquimarina

doi: 10.3390/pharmaceutics15082139

Figure Lengend Snippet: Checkerboard assay showing the combined effect of fosfomycin and the fraction F2 on MRSA. Columns contain 2-fold serial dilution of the fraction F2, while in the rows 2-fold dilutions of fosfomycin are presented. The heatmap is expressed as a percentage of bacterial growth reduction. The red square represents the combination with the lowest FICI.

Article Snippet: Antibiotics The following antibiotics loaded on disks were utilized in the Kirby–Bauer assay: moxifloxacin (MFX) 5 µg, fosfomycin (FOT) 200 µg, erythromycin (E) 15 µg, linezolid (LZD) 10 µg, 30 µg, and tigecycline (TGC) 15 µg, purchased from Mast Group Ltd. (Bootle, UK), while ampicillin (AMP) 10 µg, cefepime (FEP) 30 µg, teicoplanin (TEC), and gentamycin (CN) 15 µg were bought from Oxoid Ltd. (Thermo Fisher Scientific, Waltham, MA, USA).

Techniques: Serial Dilution