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formamide solution 0.05  (Thermo Fisher)
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Thermo Fisher formamide solution 0.05
Paralog-specific KO phenotypes indicate functional divergence of sox10a and sox10b in cichlids. a) <t>Melanophore</t> <t>pigmentation</t> defects, particularly decreased abundance, are observed in sox10b but not in sox10a A. calliptera mutants. b) sox10b -CRISPR embryos have significantly reduced melanophore counts at 7 dpf compared with WT and control injections (boxplots showing melanophore count within the dorsal head area outlined in a.; P -values are shown for Tukey HSD). c) Representative craniofacial phenotypes of sox10a- CRISPR embryos in live animals and their corresponding cartilage preparations in lateral and ventral views. White dashed lines show the reduced frontal slope of the brain case. Arrowheads and asterisks in panels showing cartilage preparations indicate reduced and missing cartilages, respectively, in the mutants. Fractions indicate the frequency of presented phenotype across all surviving embryos on that day. Data shown are representative of at least biological replicates per target gene. Ba, branchial arches; bhy, basihyal; dpf, days postfertilization; op, orbital process; pf, pectoral fin; soc, super-orbital cartilages; WT, wild-type. Scale bars = 1 mm.
Formamide Solution 0.05, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/formamide+solution+0%2E05/pmc11558072-109-19-32?v=Thermo+Fisher
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formamide solution 0.05 - by Bioz Stars, 2026-08
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stop solution 95% formamide, edta, 0.05% bromophenol blue and 0.05% xylene cyanol ff  (Amersham Life Sciences Inc)
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Amersham Life Sciences Inc stop solution 95% formamide, edta, 0.05% bromophenol blue and 0.05% xylene cyanol ff
Paralog-specific KO phenotypes indicate functional divergence of sox10a and sox10b in cichlids. a) <t>Melanophore</t> <t>pigmentation</t> defects, particularly decreased abundance, are observed in sox10b but not in sox10a A. calliptera mutants. b) sox10b -CRISPR embryos have significantly reduced melanophore counts at 7 dpf compared with WT and control injections (boxplots showing melanophore count within the dorsal head area outlined in a.; P -values are shown for Tukey HSD). c) Representative craniofacial phenotypes of sox10a- CRISPR embryos in live animals and their corresponding cartilage preparations in lateral and ventral views. White dashed lines show the reduced frontal slope of the brain case. Arrowheads and asterisks in panels showing cartilage preparations indicate reduced and missing cartilages, respectively, in the mutants. Fractions indicate the frequency of presented phenotype across all surviving embryos on that day. Data shown are representative of at least biological replicates per target gene. Ba, branchial arches; bhy, basihyal; dpf, days postfertilization; op, orbital process; pf, pectoral fin; soc, super-orbital cartilages; WT, wild-type. Scale bars = 1 mm.
Stop Solution 95% Formamide, Edta, 0.05% Bromophenol Blue And 0.05% Xylene Cyanol Ff, supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/formamide+solution+0%2E05/us07829548-428-12-31?v=Amersham+Life+Sciences+Inc
Average 90 stars, based on 1 article reviews
stop solution 95% formamide, edta, 0.05% bromophenol blue and 0.05% xylene cyanol ff - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier
stop solution containing 95% formamide, edta, 0.05% bromophenol blue, and 0.05% xylene cyanol  (Amersham Life Sciences Inc)
90
Amersham Life Sciences Inc stop solution containing 95% formamide, edta, 0.05% bromophenol blue, and 0.05% xylene cyanol
Paralog-specific KO phenotypes indicate functional divergence of sox10a and sox10b in cichlids. a) <t>Melanophore</t> <t>pigmentation</t> defects, particularly decreased abundance, are observed in sox10b but not in sox10a A. calliptera mutants. b) sox10b -CRISPR embryos have significantly reduced melanophore counts at 7 dpf compared with WT and control injections (boxplots showing melanophore count within the dorsal head area outlined in a.; P -values are shown for Tukey HSD). c) Representative craniofacial phenotypes of sox10a- CRISPR embryos in live animals and their corresponding cartilage preparations in lateral and ventral views. White dashed lines show the reduced frontal slope of the brain case. Arrowheads and asterisks in panels showing cartilage preparations indicate reduced and missing cartilages, respectively, in the mutants. Fractions indicate the frequency of presented phenotype across all surviving embryos on that day. Data shown are representative of at least biological replicates per target gene. Ba, branchial arches; bhy, basihyal; dpf, days postfertilization; op, orbital process; pf, pectoral fin; soc, super-orbital cartilages; WT, wild-type. Scale bars = 1 mm.
Stop Solution Containing 95% Formamide, Edta, 0.05% Bromophenol Blue, And 0.05% Xylene Cyanol, supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/formamide+solution+0%2E05/pmc00538822-111-13-24?v=Amersham+Life+Sciences+Inc
Average 90 stars, based on 1 article reviews
stop solution containing 95% formamide, edta, 0.05% bromophenol blue, and 0.05% xylene cyanol - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier

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Paralog-specific KO phenotypes indicate functional divergence of sox10a and sox10b in cichlids. a) Melanophore pigmentation defects, particularly decreased abundance, are observed in sox10b but not in sox10a A. calliptera mutants. b) sox10b -CRISPR embryos have significantly reduced melanophore counts at 7 dpf compared with WT and control injections (boxplots showing melanophore count within the dorsal head area outlined in a.; P -values are shown for Tukey HSD). c) Representative craniofacial phenotypes of sox10a- CRISPR embryos in live animals and their corresponding cartilage preparations in lateral and ventral views. White dashed lines show the reduced frontal slope of the brain case. Arrowheads and asterisks in panels showing cartilage preparations indicate reduced and missing cartilages, respectively, in the mutants. Fractions indicate the frequency of presented phenotype across all surviving embryos on that day. Data shown are representative of at least biological replicates per target gene. Ba, branchial arches; bhy, basihyal; dpf, days postfertilization; op, orbital process; pf, pectoral fin; soc, super-orbital cartilages; WT, wild-type. Scale bars = 1 mm.

Journal: Molecular Biology and Evolution

Article Title: Genetic and Developmental Divergence in the Neural Crest Program between Cichlid Fish Species

doi: 10.1093/molbev/msae217

Figure Lengend Snippet: Paralog-specific KO phenotypes indicate functional divergence of sox10a and sox10b in cichlids. a) Melanophore pigmentation defects, particularly decreased abundance, are observed in sox10b but not in sox10a A. calliptera mutants. b) sox10b -CRISPR embryos have significantly reduced melanophore counts at 7 dpf compared with WT and control injections (boxplots showing melanophore count within the dorsal head area outlined in a.; P -values are shown for Tukey HSD). c) Representative craniofacial phenotypes of sox10a- CRISPR embryos in live animals and their corresponding cartilage preparations in lateral and ventral views. White dashed lines show the reduced frontal slope of the brain case. Arrowheads and asterisks in panels showing cartilage preparations indicate reduced and missing cartilages, respectively, in the mutants. Fractions indicate the frequency of presented phenotype across all surviving embryos on that day. Data shown are representative of at least biological replicates per target gene. Ba, branchial arches; bhy, basihyal; dpf, days postfertilization; op, orbital process; pf, pectoral fin; soc, super-orbital cartilages; WT, wild-type. Scale bars = 1 mm.

Article Snippet: Embryos were stained for cartilage following the protocol of with following modifications: (i) all specimens were bleached to remove melanophore pigmentation using a solution of 0.05% hydrogen peroxide (Sigma) and 0.05% formamide (ThermoFisher) for 30 to 45 min under light and (ii) samples were cleared using first 50% then 70% glycerol:water solutions until complete sinking.

Techniques: Functional Assay, CRISPR, Control