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top/fop-flash luciferase reporter vector  (Millipore)
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Millipore top/fop-flash luciferase reporter vector
Top/Fop Flash Luciferase Reporter Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fop flash reporter vector  (Beyotime)
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Beyotime fop flash reporter vector
The suppressive effect of EFNA4 on dCK depends on the β-catenin signaling activation. A, protein level of β-catenin in in BxPC-3/GEM and PANC-1/GEM cells after hyperthermia examined by WB analysis (uncropped images of blots are provided as Supplementary material); B, transcriptional activity of β-catenin in cells after hyperthermia determined <t>by</t> <t>TOP</t> <t>Flash/FOP</t> Flash luciferase reporter assay; C, transcriptional activity of β-catenin in cells after EFNA4 overexpression and MSAB treatment determined by TOP Flash/FOP Flash luciferase reporter assay; D, protein levels of β-catenin and dCK in cells after EFNA4 overexpression and MSAB treatment determined by WB analysis (uncropped images of blots are provided as Supplementary material). Three biological replicates were performed. Differences were compared by the two-way ANOVA followed by Sidak's (A–B) or Tukey's (C–D) multiple comparison test. * p < 0.05.
Fop Flash Reporter Vector, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fop+flash+vector/pmc10999932-84-19-29?v=Beyotime
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fop flash reporter vector - by Bioz Stars, 2026-07
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fop-flash vector  (Upstate Biotechnology Inc)
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Upstate Biotechnology Inc fop-flash vector
The suppressive effect of EFNA4 on dCK depends on the β-catenin signaling activation. A, protein level of β-catenin in in BxPC-3/GEM and PANC-1/GEM cells after hyperthermia examined by WB analysis (uncropped images of blots are provided as Supplementary material); B, transcriptional activity of β-catenin in cells after hyperthermia determined <t>by</t> <t>TOP</t> <t>Flash/FOP</t> Flash luciferase reporter assay; C, transcriptional activity of β-catenin in cells after EFNA4 overexpression and MSAB treatment determined by TOP Flash/FOP Flash luciferase reporter assay; D, protein levels of β-catenin and dCK in cells after EFNA4 overexpression and MSAB treatment determined by WB analysis (uncropped images of blots are provided as Supplementary material). Three biological replicates were performed. Differences were compared by the two-way ANOVA followed by Sidak's (A–B) or Tukey's (C–D) multiple comparison test. * p < 0.05.
Fop Flash Vector, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fop+flash+vector/ppr0757058-128-22-24?v=Upstate+Biotechnology+Inc
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fop-flash vector - by Bioz Stars, 2026-07
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fop flash luciferase reporter vector  (BioVector NTCC)
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BioVector NTCC fop flash luciferase reporter vector
The suppressive effect of EFNA4 on dCK depends on the β-catenin signaling activation. A, protein level of β-catenin in in BxPC-3/GEM and PANC-1/GEM cells after hyperthermia examined by WB analysis (uncropped images of blots are provided as Supplementary material); B, transcriptional activity of β-catenin in cells after hyperthermia determined <t>by</t> <t>TOP</t> <t>Flash/FOP</t> Flash luciferase reporter assay; C, transcriptional activity of β-catenin in cells after EFNA4 overexpression and MSAB treatment determined by TOP Flash/FOP Flash luciferase reporter assay; D, protein levels of β-catenin and dCK in cells after EFNA4 overexpression and MSAB treatment determined by WB analysis (uncropped images of blots are provided as Supplementary material). Three biological replicates were performed. Differences were compared by the two-way ANOVA followed by Sidak's (A–B) or Tukey's (C–D) multiple comparison test. * p < 0.05.
Fop Flash Luciferase Reporter Vector, supplied by BioVector NTCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fop+flash+vector/pmc06010684-63-4-20?v=BioVector+NTCC
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fop flash luciferase reporter vector - by Bioz Stars, 2026-07
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top and fop-flash firefly luciferase vectors  (Genechem)
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Genechem top and fop-flash firefly luciferase vectors
The suppressive effect of EFNA4 on dCK depends on the β-catenin signaling activation. A, protein level of β-catenin in in BxPC-3/GEM and PANC-1/GEM cells after hyperthermia examined by WB analysis (uncropped images of blots are provided as Supplementary material); B, transcriptional activity of β-catenin in cells after hyperthermia determined <t>by</t> <t>TOP</t> <t>Flash/FOP</t> Flash luciferase reporter assay; C, transcriptional activity of β-catenin in cells after EFNA4 overexpression and MSAB treatment determined by TOP Flash/FOP Flash luciferase reporter assay; D, protein levels of β-catenin and dCK in cells after EFNA4 overexpression and MSAB treatment determined by WB analysis (uncropped images of blots are provided as Supplementary material). Three biological replicates were performed. Differences were compared by the two-way ANOVA followed by Sidak's (A–B) or Tukey's (C–D) multiple comparison test. * p < 0.05.
Top And Fop Flash Firefly Luciferase Vectors, supplied by Genechem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fop+flash+vector/pm35033815-93-0-6?v=Genechem
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top and fop-flash firefly luciferase vectors - by Bioz Stars, 2026-07
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top flash/fop flash reporter vectors  (Addgene inc)
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Addgene inc top flash/fop flash reporter vectors
The suppressive effect of EFNA4 on dCK depends on the β-catenin signaling activation. A, protein level of β-catenin in in BxPC-3/GEM and PANC-1/GEM cells after hyperthermia examined by WB analysis (uncropped images of blots are provided as Supplementary material); B, transcriptional activity of β-catenin in cells after hyperthermia determined <t>by</t> <t>TOP</t> <t>Flash/FOP</t> Flash luciferase reporter assay; C, transcriptional activity of β-catenin in cells after EFNA4 overexpression and MSAB treatment determined by TOP Flash/FOP Flash luciferase reporter assay; D, protein levels of β-catenin and dCK in cells after EFNA4 overexpression and MSAB treatment determined by WB analysis (uncropped images of blots are provided as Supplementary material). Three biological replicates were performed. Differences were compared by the two-way ANOVA followed by Sidak's (A–B) or Tukey's (C–D) multiple comparison test. * p < 0.05.
Top Flash/Fop Flash Reporter Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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top/fop-flash reporter vectors  (Addgene inc)
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Addgene inc top/fop-flash reporter vectors
EGFR-AS1 activates ACTN4-mediated WNT pathway in CC cells. a The knockdown efficacy of sh-ACTN4#1/2 on was tested by qRT-PCR in CC cells. b Expression levels of WNT pathway downstream genes in sh-ACTN4#1 transfected cells were detected by qRT-PCR in CC cells. c The effect of ACTN4 deficiency on the nuclear translocation of CTNNB1 (β-catenin) was detected by subcellular fractionation assay. d <t>TOP/FOP</t> flash assay was performed to measure the activity of WNT pathway upon ACTN4 knockdown. e , f Detection of mRNA and protein levels of WNT pathway downstream genes in CC cells transfected with sh-EGFR-AS1#1 was detected by qRT-PCR and western blot assays. g , h The nuclear translocation of CTNNB1 (β-catenin) was observed in sh-EGFR-AS1#1 group and sh-NC group. i Impact of EGFR-AS1 silencing on WNT pathway activity was detected. j – l Cell proliferation, apoptosis and invasion in cell transfected with sh-NC, sh-EGFR-AS1#1 and sh-EGFR-AS1#1 + LiCl were assessed by EdU, flow cytometry and transwell assays. **P < 0.01
Top/Fop Flash Reporter Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fop+flash+vector/pmc08742952-186-1-16?v=Addgene+inc
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top/fop-flash reporter vectors - by Bioz Stars, 2026-07
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β-catenin/tcf firefly luciferase reporter vectors fop-flash  (Promega)
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Promega β-catenin/tcf firefly luciferase reporter vectors fop-flash
EGFR-AS1 activates ACTN4-mediated WNT pathway in CC cells. a The knockdown efficacy of sh-ACTN4#1/2 on was tested by qRT-PCR in CC cells. b Expression levels of WNT pathway downstream genes in sh-ACTN4#1 transfected cells were detected by qRT-PCR in CC cells. c The effect of ACTN4 deficiency on the nuclear translocation of CTNNB1 (β-catenin) was detected by subcellular fractionation assay. d <t>TOP/FOP</t> flash assay was performed to measure the activity of WNT pathway upon ACTN4 knockdown. e , f Detection of mRNA and protein levels of WNT pathway downstream genes in CC cells transfected with sh-EGFR-AS1#1 was detected by qRT-PCR and western blot assays. g , h The nuclear translocation of CTNNB1 (β-catenin) was observed in sh-EGFR-AS1#1 group and sh-NC group. i Impact of EGFR-AS1 silencing on WNT pathway activity was detected. j – l Cell proliferation, apoptosis and invasion in cell transfected with sh-NC, sh-EGFR-AS1#1 and sh-EGFR-AS1#1 + LiCl were assessed by EdU, flow cytometry and transwell assays. **P < 0.01
β Catenin/Tcf Firefly Luciferase Reporter Vectors Fop Flash, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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top/fop-flash vectors  (Promega)
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Promega top/fop-flash vectors
EGFR-AS1 activates ACTN4-mediated WNT pathway in CC cells. a The knockdown efficacy of sh-ACTN4#1/2 on was tested by qRT-PCR in CC cells. b Expression levels of WNT pathway downstream genes in sh-ACTN4#1 transfected cells were detected by qRT-PCR in CC cells. c The effect of ACTN4 deficiency on the nuclear translocation of CTNNB1 (β-catenin) was detected by subcellular fractionation assay. d <t>TOP/FOP</t> flash assay was performed to measure the activity of WNT pathway upon ACTN4 knockdown. e , f Detection of mRNA and protein levels of WNT pathway downstream genes in CC cells transfected with sh-EGFR-AS1#1 was detected by qRT-PCR and western blot assays. g , h The nuclear translocation of CTNNB1 (β-catenin) was observed in sh-EGFR-AS1#1 group and sh-NC group. i Impact of EGFR-AS1 silencing on WNT pathway activity was detected. j – l Cell proliferation, apoptosis and invasion in cell transfected with sh-NC, sh-EGFR-AS1#1 and sh-EGFR-AS1#1 + LiCl were assessed by EdU, flow cytometry and transwell assays. **P < 0.01
Top/Fop Flash Vectors, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fop flash luciferase reporter vector  (Genechem)
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Genechem fop flash luciferase reporter vector
EGFR-AS1 activates ACTN4-mediated WNT pathway in CC cells. a The knockdown efficacy of sh-ACTN4#1/2 on was tested by qRT-PCR in CC cells. b Expression levels of WNT pathway downstream genes in sh-ACTN4#1 transfected cells were detected by qRT-PCR in CC cells. c The effect of ACTN4 deficiency on the nuclear translocation of CTNNB1 (β-catenin) was detected by subcellular fractionation assay. d <t>TOP/FOP</t> flash assay was performed to measure the activity of WNT pathway upon ACTN4 knockdown. e , f Detection of mRNA and protein levels of WNT pathway downstream genes in CC cells transfected with sh-EGFR-AS1#1 was detected by qRT-PCR and western blot assays. g , h The nuclear translocation of CTNNB1 (β-catenin) was observed in sh-EGFR-AS1#1 group and sh-NC group. i Impact of EGFR-AS1 silencing on WNT pathway activity was detected. j – l Cell proliferation, apoptosis and invasion in cell transfected with sh-NC, sh-EGFR-AS1#1 and sh-EGFR-AS1#1 + LiCl were assessed by EdU, flow cytometry and transwell assays. **P < 0.01
Fop Flash Luciferase Reporter Vector, supplied by Genechem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fop flash luciferase reporter vector - by Bioz Stars, 2026-07
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The suppressive effect of EFNA4 on dCK depends on the β-catenin signaling activation. A, protein level of β-catenin in in BxPC-3/GEM and PANC-1/GEM cells after hyperthermia examined by WB analysis (uncropped images of blots are provided as Supplementary material); B, transcriptional activity of β-catenin in cells after hyperthermia determined by TOP Flash/FOP Flash luciferase reporter assay; C, transcriptional activity of β-catenin in cells after EFNA4 overexpression and MSAB treatment determined by TOP Flash/FOP Flash luciferase reporter assay; D, protein levels of β-catenin and dCK in cells after EFNA4 overexpression and MSAB treatment determined by WB analysis (uncropped images of blots are provided as Supplementary material). Three biological replicates were performed. Differences were compared by the two-way ANOVA followed by Sidak's (A–B) or Tukey's (C–D) multiple comparison test. * p < 0.05.

Journal: Heliyon

Article Title: Hyperthermia improves gemcitabine sensitivity of pancreatic cancer cells by suppressing the EFNA4/β-catenin axis and activating dCK

doi: 10.1016/j.heliyon.2024.e28488

Figure Lengend Snippet: The suppressive effect of EFNA4 on dCK depends on the β-catenin signaling activation. A, protein level of β-catenin in in BxPC-3/GEM and PANC-1/GEM cells after hyperthermia examined by WB analysis (uncropped images of blots are provided as Supplementary material); B, transcriptional activity of β-catenin in cells after hyperthermia determined by TOP Flash/FOP Flash luciferase reporter assay; C, transcriptional activity of β-catenin in cells after EFNA4 overexpression and MSAB treatment determined by TOP Flash/FOP Flash luciferase reporter assay; D, protein levels of β-catenin and dCK in cells after EFNA4 overexpression and MSAB treatment determined by WB analysis (uncropped images of blots are provided as Supplementary material). Three biological replicates were performed. Differences were compared by the two-way ANOVA followed by Sidak's (A–B) or Tukey's (C–D) multiple comparison test. * p < 0.05.

Article Snippet: To assess the β-catenin-mediated transcriptional activity of TCF/LEF, the TOP Flash reporter vector (D2501, Beyotime) was utilized, with the FOP Flash reporter vector containing mutant TCF/LEF binding sequences (D2503, Beyotime) serving as the NC.

Techniques: Activation Assay, Activity Assay, Luciferase, Reporter Assay, Over Expression, Comparison

EGFR-AS1 activates ACTN4-mediated WNT pathway in CC cells. a The knockdown efficacy of sh-ACTN4#1/2 on was tested by qRT-PCR in CC cells. b Expression levels of WNT pathway downstream genes in sh-ACTN4#1 transfected cells were detected by qRT-PCR in CC cells. c The effect of ACTN4 deficiency on the nuclear translocation of CTNNB1 (β-catenin) was detected by subcellular fractionation assay. d TOP/FOP flash assay was performed to measure the activity of WNT pathway upon ACTN4 knockdown. e , f Detection of mRNA and protein levels of WNT pathway downstream genes in CC cells transfected with sh-EGFR-AS1#1 was detected by qRT-PCR and western blot assays. g , h The nuclear translocation of CTNNB1 (β-catenin) was observed in sh-EGFR-AS1#1 group and sh-NC group. i Impact of EGFR-AS1 silencing on WNT pathway activity was detected. j – l Cell proliferation, apoptosis and invasion in cell transfected with sh-NC, sh-EGFR-AS1#1 and sh-EGFR-AS1#1 + LiCl were assessed by EdU, flow cytometry and transwell assays. **P < 0.01

Journal: Biology Direct

Article Title: H3K27ac-activated EGFR-AS1 promotes cell growth in cervical cancer through ACTN4-mediated WNT pathway

doi: 10.1186/s13062-021-00315-5

Figure Lengend Snippet: EGFR-AS1 activates ACTN4-mediated WNT pathway in CC cells. a The knockdown efficacy of sh-ACTN4#1/2 on was tested by qRT-PCR in CC cells. b Expression levels of WNT pathway downstream genes in sh-ACTN4#1 transfected cells were detected by qRT-PCR in CC cells. c The effect of ACTN4 deficiency on the nuclear translocation of CTNNB1 (β-catenin) was detected by subcellular fractionation assay. d TOP/FOP flash assay was performed to measure the activity of WNT pathway upon ACTN4 knockdown. e , f Detection of mRNA and protein levels of WNT pathway downstream genes in CC cells transfected with sh-EGFR-AS1#1 was detected by qRT-PCR and western blot assays. g , h The nuclear translocation of CTNNB1 (β-catenin) was observed in sh-EGFR-AS1#1 group and sh-NC group. i Impact of EGFR-AS1 silencing on WNT pathway activity was detected. j – l Cell proliferation, apoptosis and invasion in cell transfected with sh-NC, sh-EGFR-AS1#1 and sh-EGFR-AS1#1 + LiCl were assessed by EdU, flow cytometry and transwell assays. **P < 0.01

Article Snippet: Besides, TOP/FOP-flash reporter vectors were available to assay the Wnt/β-catenin signaling activity as guided by manufacturer (Addgene, Cambridge, MA).

Techniques: Quantitative RT-PCR, Expressing, Transfection, Translocation Assay, Fractionation, Activity Assay, Western Blot, Flow Cytometry