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Flow cytometry characterization of hSCs (A) Color dot plot in which P2 area (24.03%) represents the cell population analyzed. SSC-H: side scatter histogram; FSC-H: forward scatter histogram. (B) CD271+ hSCs percentage (65.77%) after 1 week in culture, before purification. Surface staining for IgG was used as a reference. <t>APC-H:</t> <t>allophycocyanin</t> <t>H</t> (APC-H) fluoroprotein. (C and D) The percentage of CD271+ hSCs in purified (85.89%) and residual cell culture, respectively. (E) Histogram representation of the different percentages of CD271+ hSCs before and after purification with QuadroMACS; residual hSCs are shown. Values of columns are means ± SEM ( n = 3). One-way ANOVA using Sidak’s post-hoc test. ∗∗∗ p < 0.001.
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Flow cytometry characterization of hSCs (A) Color dot plot in which P2 area (24.03%) represents the cell population analyzed. SSC-H: side scatter histogram; FSC-H: forward scatter histogram. (B) CD271+ hSCs percentage (65.77%) after 1 week in culture, before purification. Surface staining for IgG was used as a reference. <t>APC-H:</t> <t>allophycocyanin</t> <t>H</t> (APC-H) fluoroprotein. (C and D) The percentage of CD271+ hSCs in purified (85.89%) and residual cell culture, respectively. (E) Histogram representation of the different percentages of CD271+ hSCs before and after purification with QuadroMACS; residual hSCs are shown. Values of columns are means ± SEM ( n = 3). One-way ANOVA using Sidak’s post-hoc test. ∗∗∗ p < 0.001.
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Flow cytometry characterization of hSCs (A) Color dot plot in which P2 area (24.03%) represents the cell population analyzed. SSC-H: side scatter histogram; FSC-H: forward scatter histogram. (B) CD271+ hSCs percentage (65.77%) after 1 week in culture, before purification. Surface staining for IgG was used as a reference. APC-H: allophycocyanin H (APC-H) fluoroprotein. (C and D) The percentage of CD271+ hSCs in purified (85.89%) and residual cell culture, respectively. (E) Histogram representation of the different percentages of CD271+ hSCs before and after purification with QuadroMACS; residual hSCs are shown. Values of columns are means ± SEM ( n = 3). One-way ANOVA using Sidak’s post-hoc test. ∗∗∗ p < 0.001.

Journal: iScience

Article Title: Electromagnetic exposure changes human Schwann cell motility and transcriptomic profile of hearing-loss-related genes

doi: 10.1016/j.isci.2026.115130

Figure Lengend Snippet: Flow cytometry characterization of hSCs (A) Color dot plot in which P2 area (24.03%) represents the cell population analyzed. SSC-H: side scatter histogram; FSC-H: forward scatter histogram. (B) CD271+ hSCs percentage (65.77%) after 1 week in culture, before purification. Surface staining for IgG was used as a reference. APC-H: allophycocyanin H (APC-H) fluoroprotein. (C and D) The percentage of CD271+ hSCs in purified (85.89%) and residual cell culture, respectively. (E) Histogram representation of the different percentages of CD271+ hSCs before and after purification with QuadroMACS; residual hSCs are shown. Values of columns are means ± SEM ( n = 3). One-way ANOVA using Sidak’s post-hoc test. ∗∗∗ p < 0.001.

Article Snippet: Fluorescence of the allophycocyanin H (APC-H) fluorochrome was measured using a Novocyte 3000 flow cytometer (Agilent Technologies, Santa Clara, CA, USA), acquiring at least 10,000 events per sample, with a maximum volume of 70 μL.

Techniques: Flow Cytometry, Purification, Staining, Cell Culture