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inverted fluorescence microscope  (Nikon)


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    Structured Review

    Nikon inverted fluorescence microscope
    Inverted Fluorescence Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 14721 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluorescence+inverted+microscope/Inverted+Microscopes/pmc12857289-402-1-4
    Average 99 stars, based on 14721 article reviews
    inverted fluorescence microscope - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Fluorescence:

    Article Title: STIM1-containing contact sites promote direct calcium flux from the endoplasmic reticulum to mitochondria.
    Article Snippet: Then, cells were washed twice with HBSS on a heated plate to ensure temperature stability at 37 °C and placed in a DH-40i micro-incubation platform of an inverted Nikon Ti-E microscope. .. After 10min, 5 images were captured with a CCD Hamamatsu C9100-02 camera coupled to the fluorescence inverted microscope, using the G-2A filter block from Nikon, which includes a 510–560 nm excitation filter, a 565-nm dichroic mirror, and a 590-nm emission filter. ..

    Article Title: A rapid and selective phenothiazine-derived fluorescent probe for hypochlorous acid monitoring in living systems: from cellular imaging to in vivo systemic inflammatory response syndrome visualization.
    Article Snippet: Hypochlorous acid (HClO) is a pivotal constituent of the reactive oxygen species (ROS) family, playing a pivotal role in physiological processes such as immune defence and cellular signalling.. However, it is important to note that excessive hypochlorous acid production is closely associated with various pathological conditions, including inflammation, neurodegenerative diseases, and cancer.. Consequently, the development of tools capable of realtime, highly selective, and sensitive detection of HClO in biological systems is imperative for enhancing our comprehension of its mechanisms in life processes and facilitating early diagnosis of related diseases.

    Article Title: Simultaneous improvement of breast muscle yield and meat quality in Langshan chickens
    Article Snippet: The secondary antibodies used in the immunofluorescence test are as follows: CY3-labeled goat anti-mouse IgG (Beyotime Biotechnology, Shanghai, China), Alexa Fluor® 488-conjugated goat anti-rabbit IgG (Beyotime Biotechnology). .. Images were acquired by using a Nikon Eclipse Ti-U fluorescence inverted microscope (Nikon Instruments, InC., Mellville, NY) at 200 × magnification. ..

    Article Title: METTL14-mediated RNA m 6 A modifications of TET1 and TET2 in duck myoblast differentiation
    Article Snippet: For the EdU assay, DPMs were plated in 48-well plates and stained 24 hours post-transfection using an EdU kit (MeilunBio). .. Each group consisted of six replicates, with three images taken at different locations for each replicate via a fluorescence inverted microscope (TS2R-FL, Nikon, Tokyo, Japan) to quantify the number of EdU-positive cells and calculate the ratio of the number of EdU-positive cells to the total number of nuclei stained with DAPI (Servicebio, Wuhan, China). .. Cell proteins were extracted via RIPA lysis buffer (Beyotime, Shanghai, China) containing 1% PMSF (Beyotime).

    Article Title: Molecular characterization of rabbit hemorrhagic disease virus type 2 and long-lasting protection conferred by a VLP-based vaccine.
    Article Snippet: A fluorescein isothiocyanate-conjugated goat anti-rabbit secondary antibody (Invitrogen, USA, Cat No. 10359016A-11008) was then applied. .. Samples were observed under a fluorescence inverted microscope (TS2-FL, Nikon). ..

    Article Title: Acute triclosan exposure induces multidimensional toxicity in zebrafish larvae: Disruption of development, behavior, immune function, and cell death mechanisms.
    Article Snippet: Triclosan (TCS), a widely used antibacterial agent, is frequently detected in aquatic environments, raising concerns about its ecological toxicity.. However, the mechanisms underlying TCS-induced developmental toxicity, neurobehavioral impairment, immune dysfunction, and cell death pathways remain insufficiently understood.. In this study, zebrafish larvae were exposed to TCS at 2.3, 23, and 230 μg/L to systematically evaluate its effects on development, behavior, oxidative stress, immune responses, and cell death-related pathways.

    Article Title: PANoptosis inhibition by Morchella sextelata polysaccharide mediates neuroprotection in Alzheimer's disease models.
    Article Snippet: PANoptosis has been hypothesized to play a role in the pathogenesis of Alzheimer's disease (AD), with potential contributions to neuronal dysfunction and loss.. This study aims to analyze the structural characteristics of Morchella sextelata polysaccharide (MSP) and to investigate its neuroprotective properties and the pathways implicated in AD pathology.. The main chain of the MSP polysaccharide, with a molecular weight of 2283.3 kDa, consists of →4)-α-D-Glcp-(1 → and →4,6)-β-D-Glcp-(1 → linkages, and α-D-Glcp-(1 → connected as a branched chain to the O-4 position of →4,6)-β-D-Glcp-(1→.

    Article Title: Identification of a novel EphB4 inhibitor, Sanguinarine, which attenuates β-catenin signaling to inhibit tumor proliferation and migration in lung cancer.
    Article Snippet: .. Images of the stained colonies were captured using a fluorescence-inverted microscope (DM505, Nikon Co., Ltd, Japan). ..

    Inverted Microscopy:

    Article Title: STIM1-containing contact sites promote direct calcium flux from the endoplasmic reticulum to mitochondria.
    Article Snippet: Then, cells were washed twice with HBSS on a heated plate to ensure temperature stability at 37 °C and placed in a DH-40i micro-incubation platform of an inverted Nikon Ti-E microscope. .. After 10min, 5 images were captured with a CCD Hamamatsu C9100-02 camera coupled to the fluorescence inverted microscope, using the G-2A filter block from Nikon, which includes a 510–560 nm excitation filter, a 565-nm dichroic mirror, and a 590-nm emission filter. ..

    Article Title: A rapid and selective phenothiazine-derived fluorescent probe for hypochlorous acid monitoring in living systems: from cellular imaging to in vivo systemic inflammatory response syndrome visualization.
    Article Snippet: Hypochlorous acid (HClO) is a pivotal constituent of the reactive oxygen species (ROS) family, playing a pivotal role in physiological processes such as immune defence and cellular signalling.. However, it is important to note that excessive hypochlorous acid production is closely associated with various pathological conditions, including inflammation, neurodegenerative diseases, and cancer.. Consequently, the development of tools capable of realtime, highly selective, and sensitive detection of HClO in biological systems is imperative for enhancing our comprehension of its mechanisms in life processes and facilitating early diagnosis of related diseases.

    Article Title: Simultaneous improvement of breast muscle yield and meat quality in Langshan chickens
    Article Snippet: The secondary antibodies used in the immunofluorescence test are as follows: CY3-labeled goat anti-mouse IgG (Beyotime Biotechnology, Shanghai, China), Alexa Fluor® 488-conjugated goat anti-rabbit IgG (Beyotime Biotechnology). .. Images were acquired by using a Nikon Eclipse Ti-U fluorescence inverted microscope (Nikon Instruments, InC., Mellville, NY) at 200 × magnification. ..

    Article Title: METTL14-mediated RNA m 6 A modifications of TET1 and TET2 in duck myoblast differentiation
    Article Snippet: For the EdU assay, DPMs were plated in 48-well plates and stained 24 hours post-transfection using an EdU kit (MeilunBio). .. Each group consisted of six replicates, with three images taken at different locations for each replicate via a fluorescence inverted microscope (TS2R-FL, Nikon, Tokyo, Japan) to quantify the number of EdU-positive cells and calculate the ratio of the number of EdU-positive cells to the total number of nuclei stained with DAPI (Servicebio, Wuhan, China). .. Cell proteins were extracted via RIPA lysis buffer (Beyotime, Shanghai, China) containing 1% PMSF (Beyotime).

    Article Title: Molecular characterization of rabbit hemorrhagic disease virus type 2 and long-lasting protection conferred by a VLP-based vaccine.
    Article Snippet: A fluorescein isothiocyanate-conjugated goat anti-rabbit secondary antibody (Invitrogen, USA, Cat No. 10359016A-11008) was then applied. .. Samples were observed under a fluorescence inverted microscope (TS2-FL, Nikon). ..

    Article Title: Acute triclosan exposure induces multidimensional toxicity in zebrafish larvae: Disruption of development, behavior, immune function, and cell death mechanisms.
    Article Snippet: Triclosan (TCS), a widely used antibacterial agent, is frequently detected in aquatic environments, raising concerns about its ecological toxicity.. However, the mechanisms underlying TCS-induced developmental toxicity, neurobehavioral impairment, immune dysfunction, and cell death pathways remain insufficiently understood.. In this study, zebrafish larvae were exposed to TCS at 2.3, 23, and 230 μg/L to systematically evaluate its effects on development, behavior, oxidative stress, immune responses, and cell death-related pathways.

    Article Title: PANoptosis inhibition by Morchella sextelata polysaccharide mediates neuroprotection in Alzheimer's disease models.
    Article Snippet: PANoptosis has been hypothesized to play a role in the pathogenesis of Alzheimer's disease (AD), with potential contributions to neuronal dysfunction and loss.. This study aims to analyze the structural characteristics of Morchella sextelata polysaccharide (MSP) and to investigate its neuroprotective properties and the pathways implicated in AD pathology.. The main chain of the MSP polysaccharide, with a molecular weight of 2283.3 kDa, consists of →4)-α-D-Glcp-(1 → and →4,6)-β-D-Glcp-(1 → linkages, and α-D-Glcp-(1 → connected as a branched chain to the O-4 position of →4,6)-β-D-Glcp-(1→.

    Blocking Assay:

    Article Title: STIM1-containing contact sites promote direct calcium flux from the endoplasmic reticulum to mitochondria.
    Article Snippet: Then, cells were washed twice with HBSS on a heated plate to ensure temperature stability at 37 °C and placed in a DH-40i micro-incubation platform of an inverted Nikon Ti-E microscope. .. After 10min, 5 images were captured with a CCD Hamamatsu C9100-02 camera coupled to the fluorescence inverted microscope, using the G-2A filter block from Nikon, which includes a 510–560 nm excitation filter, a 565-nm dichroic mirror, and a 590-nm emission filter. ..

    Imaging:

    Article Title: A rapid and selective phenothiazine-derived fluorescent probe for hypochlorous acid monitoring in living systems: from cellular imaging to in vivo systemic inflammatory response syndrome visualization.
    Article Snippet: Hypochlorous acid (HClO) is a pivotal constituent of the reactive oxygen species (ROS) family, playing a pivotal role in physiological processes such as immune defence and cellular signalling.. However, it is important to note that excessive hypochlorous acid production is closely associated with various pathological conditions, including inflammation, neurodegenerative diseases, and cancer.. Consequently, the development of tools capable of realtime, highly selective, and sensitive detection of HClO in biological systems is imperative for enhancing our comprehension of its mechanisms in life processes and facilitating early diagnosis of related diseases.

    Staining:

    Article Title: METTL14-mediated RNA m 6 A modifications of TET1 and TET2 in duck myoblast differentiation
    Article Snippet: For the EdU assay, DPMs were plated in 48-well plates and stained 24 hours post-transfection using an EdU kit (MeilunBio). .. Each group consisted of six replicates, with three images taken at different locations for each replicate via a fluorescence inverted microscope (TS2R-FL, Nikon, Tokyo, Japan) to quantify the number of EdU-positive cells and calculate the ratio of the number of EdU-positive cells to the total number of nuclei stained with DAPI (Servicebio, Wuhan, China). .. Cell proteins were extracted via RIPA lysis buffer (Beyotime, Shanghai, China) containing 1% PMSF (Beyotime).

    Article Title: Identification of a novel EphB4 inhibitor, Sanguinarine, which attenuates β-catenin signaling to inhibit tumor proliferation and migration in lung cancer.
    Article Snippet: .. Images of the stained colonies were captured using a fluorescence-inverted microscope (DM505, Nikon Co., Ltd, Japan). ..

    Microscopy:

    Article Title: Acute triclosan exposure induces multidimensional toxicity in zebrafish larvae: Disruption of development, behavior, immune function, and cell death mechanisms.
    Article Snippet: Triclosan (TCS), a widely used antibacterial agent, is frequently detected in aquatic environments, raising concerns about its ecological toxicity.. However, the mechanisms underlying TCS-induced developmental toxicity, neurobehavioral impairment, immune dysfunction, and cell death pathways remain insufficiently understood.. In this study, zebrafish larvae were exposed to TCS at 2.3, 23, and 230 μg/L to systematically evaluate its effects on development, behavior, oxidative stress, immune responses, and cell death-related pathways.

    Article Title: Identification of a novel EphB4 inhibitor, Sanguinarine, which attenuates β-catenin signaling to inhibit tumor proliferation and migration in lung cancer.
    Article Snippet: .. Images of the stained colonies were captured using a fluorescence-inverted microscope (DM505, Nikon Co., Ltd, Japan). ..

    Incubation:

    Article Title: PANoptosis inhibition by Morchella sextelata polysaccharide mediates neuroprotection in Alzheimer's disease models.
    Article Snippet: PANoptosis has been hypothesized to play a role in the pathogenesis of Alzheimer's disease (AD), with potential contributions to neuronal dysfunction and loss.. This study aims to analyze the structural characteristics of Morchella sextelata polysaccharide (MSP) and to investigate its neuroprotective properties and the pathways implicated in AD pathology.. The main chain of the MSP polysaccharide, with a molecular weight of 2283.3 kDa, consists of →4)-α-D-Glcp-(1 → and →4,6)-β-D-Glcp-(1 → linkages, and α-D-Glcp-(1 → connected as a branched chain to the O-4 position of →4,6)-β-D-Glcp-(1→.



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