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Immunophenotyping and differentiation potentials of WJ-MSCs. (A) WJ-MSCs in the Flow <t>cytometry</t> system, express CD44 and CD105, and are negative CD34 and CD45 markers. (B) Oil Red O staining of WJ-MSCs (C) Alizarin Red S staining of WJ-MSCs
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miRNA transfection in human umbilical vein endothelial cells. The PEI particles containing FITC-conjugated miRNA were transfected into cells and the delivery levels were estimated up to 98% using flow <t>cytometry.</t> Scattered light plot of SSC (side scatter) vs FSC (forward scatter). FL1 (fluorescence channel 1) vs. FL2 (orange channel 2) scatter and count plots
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Immunophenotyping and differentiation potentials of WJ-MSCs. (A) WJ-MSCs in the Flow cytometry system, express CD44 and CD105, and are negative CD34 and CD45 markers. (B) Oil Red O staining of WJ-MSCs (C) Alizarin Red S staining of WJ-MSCs

Journal: Iranian Journal of Basic Medical Sciences

Article Title: Exosomes of Whartons’ jelly mesenchymal stem cell reduce the NOX genes in TGF-β-induced hepatic fibrosis

doi: 10.22038/IJBMS.2022.66802.14649

Figure Lengend Snippet: Immunophenotyping and differentiation potentials of WJ-MSCs. (A) WJ-MSCs in the Flow cytometry system, express CD44 and CD105, and are negative CD34 and CD45 markers. (B) Oil Red O staining of WJ-MSCs (C) Alizarin Red S staining of WJ-MSCs

Article Snippet: The required extracted cells were examined using the well-known flow cytometry technique (Becton Dickinson, USA), and finally, the data was evaluated using FlowJo analyzer software.

Techniques: Flow Cytometry, Staining

miRNA transfection in human umbilical vein endothelial cells. The PEI particles containing FITC-conjugated miRNA were transfected into cells and the delivery levels were estimated up to 98% using flow cytometry. Scattered light plot of SSC (side scatter) vs FSC (forward scatter). FL1 (fluorescence channel 1) vs. FL2 (orange channel 2) scatter and count plots

Journal: BMC Research Notes

Article Title: miR-27a inhibits molecular adhesion between monocytes and human umbilical vein endothelial cells; systemic approach

doi: 10.1186/s13104-022-05920-9

Figure Lengend Snippet: miRNA transfection in human umbilical vein endothelial cells. The PEI particles containing FITC-conjugated miRNA were transfected into cells and the delivery levels were estimated up to 98% using flow cytometry. Scattered light plot of SSC (side scatter) vs FSC (forward scatter). FL1 (fluorescence channel 1) vs. FL2 (orange channel 2) scatter and count plots

Article Snippet: After incubating the cells with PEI containing FITC-conjugated miRNA for 4 h, they were harvested and examined by flow cytometry technique (CyFlow, Partec, Germany).

Techniques: Transfection, Flow Cytometry, Fluorescence