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Characterization, cytotoxicity, uptake, and drug release of biomimetic liposomes. (A, B) Mean diameter (A) and Zeta potential (B) of PLip ( n = 3). (C) The representative Cryo-EM image depicted PLip laden with gambogic acid. Scale bar = 100 nm. (D) CCK-8 assay to evaluate the viability of 4T1 cells after various treatments ( n = 3). (E, F) Representative flow cytometry plots depicting apoptosis in tumor cells following various treatments (F), along with a corresponding statistical graph of late apoptosis (E) ( n = 3). (G, H) Observations of cellular uptake of DiD-labeled liposomes under various treatments using fluorescence confocal microscopy (G, scale bar = 20 μm), and flow cytometric quantification (H) ( n = 3). (I) Alterations of CD44 protein expression in tumor cells following HIFU treatment ( n = 3). (J) Comparison of PLip’s drug release profile in high H 2 O 2 conditions versus an H 2 O 2 -free environment ( n = 3). (K, L) Intracellular (K) and extracellular (L) H 2 O 2 levels following various treatments ( n = 3). Data are presented as mean ± SD. Statistical significance was analyzed by one-way ANOVA with a Tukey post hoc test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001 vs. indicated; ns, no significance.

Journal: Acta Pharmaceutica Sinica. B

Article Title: Biomimetic liposomes synergize with high-intensity focused ultrasound to induce ferroptosis in tumors

doi: 10.1016/j.apsb.2026.02.024

Figure Lengend Snippet: Characterization, cytotoxicity, uptake, and drug release of biomimetic liposomes. (A, B) Mean diameter (A) and Zeta potential (B) of PLip ( n = 3). (C) The representative Cryo-EM image depicted PLip laden with gambogic acid. Scale bar = 100 nm. (D) CCK-8 assay to evaluate the viability of 4T1 cells after various treatments ( n = 3). (E, F) Representative flow cytometry plots depicting apoptosis in tumor cells following various treatments (F), along with a corresponding statistical graph of late apoptosis (E) ( n = 3). (G, H) Observations of cellular uptake of DiD-labeled liposomes under various treatments using fluorescence confocal microscopy (G, scale bar = 20 μm), and flow cytometric quantification (H) ( n = 3). (I) Alterations of CD44 protein expression in tumor cells following HIFU treatment ( n = 3). (J) Comparison of PLip’s drug release profile in high H 2 O 2 conditions versus an H 2 O 2 -free environment ( n = 3). (K, L) Intracellular (K) and extracellular (L) H 2 O 2 levels following various treatments ( n = 3). Data are presented as mean ± SD. Statistical significance was analyzed by one-way ANOVA with a Tukey post hoc test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001 vs. indicated; ns, no significance.

Article Snippet: Subsequently, flow cytometric analysis was performed on the cells using FlowJo v.7.6.5 (Tree Star, Inc., Ashland, USA).

Techniques: Liposomes, Zeta Potential Analyzer, Cryo-EM Sample Prep, CCK-8 Assay, Flow Cytometry, Labeling, Fluorescence, Confocal Microscopy, Expressing, Comparison