Journal: bioRxiv

Article Title: Loss of the actin remodeling protein Flightless-1 impairs CD8 and regulatory T cell function

doi: 10.1101/2025.08.15.669900

Figure Lengend Snippet: (A) Expression of the indicated genes gelsolin domains in murine naïve CD4 + and CD8 + T cells. (B) Expression of the Flii and Gsn genes in the indicated T cell populations. Data in (A) and (B) are from Gene Expression Omnibus dataset GSE109125. (C) Schematic of FLII protein structure. (D) Western blot analysis of FLII and β-actin protein expression in the indicated murine T cell populations. Data are representative of 2 independent experiments pooling cells from 2 mice per experiment.

Article Snippet: Membranes were stained with primary antibodies against FLII (Santa Cruz) and β-actin (Cell Signaling Technology) and secondary antibodies anti-mouse IgG HRP (Invitrogen) and anti-rabbit IgG HRP (Cell Signaling Technology) before imaging on a Chemidoc (BioRad) using Immobilon Western Chemiluminescent HRP Substrate (Millipore).

Techniques: Expressing, Gene Expression, Western Blot

Journal: bioRxiv

Article Title: Loss of the actin remodeling protein Flightless-1 impairs CD8 and regulatory T cell function

doi: 10.1101/2025.08.15.669900

Figure Lengend Snippet: (A) Western blot analysis of FLII and β-actin protein expression in the indicated T cell subsets from sorted from Flii wt/wt dLck cre and Flii fl/fl dLck cre mice as indicated. (B) Representative flow cytometry staining of CD4 and CD8 expression by gated live thymocytes, and frequency of thymic T cell populations in Flii wt/wt dLck cre and Flii fl/fl dLck cre mice as indicated. (C) Representative flow cytometry staining of CD4 and CD8 by gated live TCRβ + T cells in the spleens of Flii wt/wt dLck cre and Flii fl/fl dLck cre mice as indicated, and quantification of CD8 + cell number and frequency. (D) Representative flow cytometry staining and quantification of naïve (CD44 - CD62L + ), effector (CD44 + CD62L - ), and memory (CD44 + CD62L + ) CD8 + cells from Flii wt/wt dLck cre and Flii fl/fl dLck cre mice as indicated. Data are representative of 2 independent experiments, 2-4 mice per group. Statistical analyses performed by two-way ANOVA with Sidak’s multiple comparisons test.

Article Snippet: Membranes were stained with primary antibodies against FLII (Santa Cruz) and β-actin (Cell Signaling Technology) and secondary antibodies anti-mouse IgG HRP (Invitrogen) and anti-rabbit IgG HRP (Cell Signaling Technology) before imaging on a Chemidoc (BioRad) using Immobilon Western Chemiluminescent HRP Substrate (Millipore).

Techniques: Western Blot, Expressing, Flow Cytometry, Staining