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( A ) Representative waveform traces demonstrating altered breathing patterns in SHAM and bleomycin-treated (BLEO) male and female rats in both matching approaches. ( B-G ). Non-invasive whole-body plethysmography <t>(WBP)</t> measurements showing ( B ) respiratory rate (RR, breaths/min), ( C ) tidal normalized to body weight (TV/kg, mL/kg), ( D ) minute ventilation normalized to body weight (MV/kg, ml/min/kg), ( E ) mid-tidal expiratory flow (EF50, mL/s), ( F ) enhanced pause (Penh), and ( G ) relative time to peak expiratory flow (Rpef). Invasive lung mechanics measurements of ( H ) airway resistance (RI, cmH 2 O·s/mL) and ( I ) dynamic compliance (Cdyn, mL/cmH 2 O). Data are presented as mean ± SEM. For B-G, age matched cohort: n = 10 per group; BW matched group: n = 6 males, n = 8 females. For H-I, age matched group: n = 5-7 for SHAM, n = 7-9 for BLEO; BW-matched group: n = 5-6 for SHAM, n = 7-8 for BLEO. *p < 0.05, ns = not significant. Statistical analysis performed using two-way ANOVA with Tukey’s multiple comparisons test.
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( A ) Representative waveform traces demonstrating altered breathing patterns in SHAM and bleomycin-treated (BLEO) male and female rats in both matching approaches. ( B-G ). Non-invasive whole-body plethysmography <t>(WBP)</t> measurements showing ( B ) respiratory rate (RR, breaths/min), ( C ) tidal normalized to body weight (TV/kg, mL/kg), ( D ) minute ventilation normalized to body weight (MV/kg, ml/min/kg), ( E ) mid-tidal expiratory flow (EF50, mL/s), ( F ) enhanced pause (Penh), and ( G ) relative time to peak expiratory flow (Rpef). Invasive lung mechanics measurements of ( H ) airway resistance (RI, cmH 2 O·s/mL) and ( I ) dynamic compliance (Cdyn, mL/cmH 2 O). Data are presented as mean ± SEM. For B-G, age matched cohort: n = 10 per group; BW matched group: n = 6 males, n = 8 females. For H-I, age matched group: n = 5-7 for SHAM, n = 7-9 for BLEO; BW-matched group: n = 5-6 for SHAM, n = 7-8 for BLEO. *p < 0.05, ns = not significant. Statistical analysis performed using two-way ANOVA with Tukey’s multiple comparisons test.
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( A ) Representative waveform traces demonstrating altered breathing patterns in SHAM and bleomycin-treated (BLEO) male and female rats in both matching approaches. ( B-G ). Non-invasive whole-body plethysmography <t>(WBP)</t> measurements showing ( B ) respiratory rate (RR, breaths/min), ( C ) tidal normalized to body weight (TV/kg, mL/kg), ( D ) minute ventilation normalized to body weight (MV/kg, ml/min/kg), ( E ) mid-tidal expiratory flow (EF50, mL/s), ( F ) enhanced pause (Penh), and ( G ) relative time to peak expiratory flow (Rpef). Invasive lung mechanics measurements of ( H ) airway resistance (RI, cmH 2 O·s/mL) and ( I ) dynamic compliance (Cdyn, mL/cmH 2 O). Data are presented as mean ± SEM. For B-G, age matched cohort: n = 10 per group; BW matched group: n = 6 males, n = 8 females. For H-I, age matched group: n = 5-7 for SHAM, n = 7-9 for BLEO; BW-matched group: n = 5-6 for SHAM, n = 7-8 for BLEO. *p < 0.05, ns = not significant. Statistical analysis performed using two-way ANOVA with Tukey’s multiple comparisons test.
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( A ) Representative waveform traces demonstrating altered breathing patterns in SHAM and bleomycin-treated (BLEO) male and female rats in both matching approaches. ( B-G ). Non-invasive whole-body plethysmography <t>(WBP)</t> measurements showing ( B ) respiratory rate (RR, breaths/min), ( C ) tidal normalized to body weight (TV/kg, mL/kg), ( D ) minute ventilation normalized to body weight (MV/kg, ml/min/kg), ( E ) mid-tidal expiratory flow (EF50, mL/s), ( F ) enhanced pause (Penh), and ( G ) relative time to peak expiratory flow (Rpef). Invasive lung mechanics measurements of ( H ) airway resistance (RI, cmH 2 O·s/mL) and ( I ) dynamic compliance (Cdyn, mL/cmH 2 O). Data are presented as mean ± SEM. For B-G, age matched cohort: n = 10 per group; BW matched group: n = 6 males, n = 8 females. For H-I, age matched group: n = 5-7 for SHAM, n = 7-9 for BLEO; BW-matched group: n = 5-6 for SHAM, n = 7-8 for BLEO. *p < 0.05, ns = not significant. Statistical analysis performed using two-way ANOVA with Tukey’s multiple comparisons test.
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( A ) Representative waveform traces demonstrating altered breathing patterns in SHAM and bleomycin-treated (BLEO) male and female rats in both matching approaches. ( B-G ). Non-invasive whole-body plethysmography <t>(WBP)</t> measurements showing ( B ) respiratory rate (RR, breaths/min), ( C ) tidal normalized to body weight (TV/kg, mL/kg), ( D ) minute ventilation normalized to body weight (MV/kg, ml/min/kg), ( E ) mid-tidal expiratory flow (EF50, mL/s), ( F ) enhanced pause (Penh), and ( G ) relative time to peak expiratory flow (Rpef). Invasive lung mechanics measurements of ( H ) airway resistance (RI, cmH 2 O·s/mL) and ( I ) dynamic compliance (Cdyn, mL/cmH 2 O). Data are presented as mean ± SEM. For B-G, age matched cohort: n = 10 per group; BW matched group: n = 6 males, n = 8 females. For H-I, age matched group: n = 5-7 for SHAM, n = 7-9 for BLEO; BW-matched group: n = 5-6 for SHAM, n = 7-8 for BLEO. *p < 0.05, ns = not significant. Statistical analysis performed using two-way ANOVA with Tukey’s multiple comparisons test.
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( A ) Representative waveform traces demonstrating altered breathing patterns in SHAM and bleomycin-treated (BLEO) male and female rats in both matching approaches. ( B-G ). Non-invasive whole-body plethysmography (WBP) measurements showing ( B ) respiratory rate (RR, breaths/min), ( C ) tidal normalized to body weight (TV/kg, mL/kg), ( D ) minute ventilation normalized to body weight (MV/kg, ml/min/kg), ( E ) mid-tidal expiratory flow (EF50, mL/s), ( F ) enhanced pause (Penh), and ( G ) relative time to peak expiratory flow (Rpef). Invasive lung mechanics measurements of ( H ) airway resistance (RI, cmH 2 O·s/mL) and ( I ) dynamic compliance (Cdyn, mL/cmH 2 O). Data are presented as mean ± SEM. For B-G, age matched cohort: n = 10 per group; BW matched group: n = 6 males, n = 8 females. For H-I, age matched group: n = 5-7 for SHAM, n = 7-9 for BLEO; BW-matched group: n = 5-6 for SHAM, n = 7-8 for BLEO. *p < 0.05, ns = not significant. Statistical analysis performed using two-way ANOVA with Tukey’s multiple comparisons test.

Journal: bioRxiv

Article Title: Sex-Specific Pathophysiological Signatures in Allometric Dosing-Controlled Bleomycin Acute Lung Injury Model

doi: 10.64898/2026.03.10.710851

Figure Lengend Snippet: ( A ) Representative waveform traces demonstrating altered breathing patterns in SHAM and bleomycin-treated (BLEO) male and female rats in both matching approaches. ( B-G ). Non-invasive whole-body plethysmography (WBP) measurements showing ( B ) respiratory rate (RR, breaths/min), ( C ) tidal normalized to body weight (TV/kg, mL/kg), ( D ) minute ventilation normalized to body weight (MV/kg, ml/min/kg), ( E ) mid-tidal expiratory flow (EF50, mL/s), ( F ) enhanced pause (Penh), and ( G ) relative time to peak expiratory flow (Rpef). Invasive lung mechanics measurements of ( H ) airway resistance (RI, cmH 2 O·s/mL) and ( I ) dynamic compliance (Cdyn, mL/cmH 2 O). Data are presented as mean ± SEM. For B-G, age matched cohort: n = 10 per group; BW matched group: n = 6 males, n = 8 females. For H-I, age matched group: n = 5-7 for SHAM, n = 7-9 for BLEO; BW-matched group: n = 5-6 for SHAM, n = 7-8 for BLEO. *p < 0.05, ns = not significant. Statistical analysis performed using two-way ANOVA with Tukey’s multiple comparisons test.

Article Snippet: Data was recorded using DSI FinePointe WBP software (Data Sciences International, St. Paul, MN, USA).

Techniques: